Blood Analysis of Laboratory<i>Macaca mulatta</i>Used for Neuroscience Research: Investigation of Long-Term and Cumulative Effects of Implants, Fluid Control, and Laboratory Procedures

Wegener, Detlef; Oh, Dan Qi Priscilla; Lukaß, Herbert; Boer, M.M.R.; Kreiter, Andreas K.

doi:10.1523/eneuro.0284-21.2021

Cited by 10 publications

(10 citation statements)

References 104 publications

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“…Nonhuman primates, which are homologous with humans, constitute an indispensable laboratory model for various fields of biomedical research ( Evans and Silvestri, 2013 ; Roelfsema and Treue, 2014 ; Tomalka et al., 2021 ; Wegener et al., 2021 ), and have become ideal glaucoma experimental animals because of their highly consistent anatomical structure and physiological characteristics with humans eyeballs ( Pasquale et al., 2021 ; Rasmussen and Kaufman, 2005 ; Stewart et al., 2011 ). There are several kinds of chronic ocular hypertension (COHT) model in nonhuman primates, including topical dexamethasone treatment (steroid-induced glaucoma), intracameral injection of microbeads made of different materials, and laser photocoagulation (LP) of the trabecular meshwork (TM) ( Fingert et al., 2001 ; Pelzel et al., 2006 ; Quigley and Hohman, 1983 ; Tu et al., 2019 ; Weber and Harman, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Long-term and potent IOP-lowering effect of IκBα-siRNA in a nonhuman primate model of chronic ocular hypertension

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Long-term and potent IOP-lowering effect of IκBα-siRNA in a nonhuman primate model of chronic ocular hypertension

et al. 2022

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“…Acrylic dental cement is widely successfully used ( Wegener et al, 2021 ). Nevertheless, there are still disadvantages.…”

Section: Discussionmentioning

confidence: 99%

Universal Guide for Skull Extraction and Custom-Fitting of Implants to Continuous and Discontinuous Skulls

Ahmed

Agha

Trunk

et al. 2022

eNeuro

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Intracranial neurophysiological recordings require chronic implants to provide transcranial access to the brain. Especially in larger animals, which participate in experiments over extended periods of time, implants should match the skull curvature to promote osseointegration and avoid tissue and bacterial ingress over time. Proposed CAD methods for designing implants to date have focused on naive animals with continuous and even skull surfaces and calculate Boolean differences between implant and skull surface to fit the implant to the skull curvature. However, custom-fitting by calculating the difference fails, if a discontinuous skull surface needs to be matched. Also, the difference method does not allow designs with constant material thickness along the skull curvature, e.g., to allow fixed screw lengths. We present a universal step-by-step guide for custom-fitting implants which overcomes these limitations. It is suited for unusual skull conditions, like surface discontinuities or irregularities and includes virtual bending as a process to match skull surfaces while maintaining implant thickness. We demonstrate its applicability for a wide spectrum of scenarios, ranging from complex-shaped single-pieced implants to detailed multicomponent implant systems built on even or discontinuous skull. The guide uses only a few software tools and the final virtual product can be manufactured using CNC milling or 3D printing. A detailed description of this process is available on GitHub including step-by-step video instructions suitable for users without any prior knowledge in CAD programming. We report the experience with these implants over several years in 11 rhesus monkeys.

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“…Three individuals were single-housed, with visual and auditory contact with other monkeys. A detailed description of the housing conditions can be found elsewhere 33 .…”

Section: Methodsmentioning

confidence: 99%

Monitoring and immunogenicity of SARS-CoV-2 vaccination of laboratory rhesus monkeys (Macaca mulatta)

Grothe

Lukaß

et al. 2023

Sci Rep

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The availability of effective vaccines and a high vaccination rate allowed the recent mitigation, or even withdrawal, of many protective measures for containing the SARS CoV-2 pandemic. At the same time, new and highly mutated variants of the virus are found to have significantly higher transmissibility and reduced vaccine efficacy, thus causing high infection rates during the third year of the pandemic. The combination of reduced measures and increased infectivity poses a particular risk for unvaccinated individuals, including animals susceptible to the virus. Among the latter, non-human primates (NHPs) are particularly vulnerable. They serve as important models in various fields of biomedical research and because of their cognitive capabilities, they receive particular attention in animal welfare regulations around the world. Yet, although they played an extraordinarily important role for developing and testing vaccines against SARS-CoV-2, the protection of captive rhesus monkeys against Covid-19 has rarely been discussed. We here report upon twofold mRNA vaccination of a cohort of 19 elderly rhesus monkeys (Macaca mulatta) against infection by SARS-CoV-2. All animals were closely monitored on possible side effects of vaccination, and were tested for neutralising antibodies against the virus. The data show that vaccination of rhesus monkeys is a safe and reliable measure to protect these animals against SARS-CoV-2.

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Blood Analysis of LaboratoryMacaca mulattaUsed for Neuroscience Research: Investigation of Long-Term and Cumulative Effects of Implants, Fluid Control, and Laboratory Procedures

Cited by 10 publications

References 104 publications

Long-term and potent IOP-lowering effect of IκBα-siRNA in a nonhuman primate model of chronic ocular hypertension

Long-term and potent IOP-lowering effect of IκBα-siRNA in a nonhuman primate model of chronic ocular hypertension

Universal Guide for Skull Extraction and Custom-Fitting of Implants to Continuous and Discontinuous Skulls

Monitoring and immunogenicity of SARS-CoV-2 vaccination of laboratory rhesus monkeys (Macaca mulatta)

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