Blood and other possible inflammatory cells in the sparid Acanthopagrus australis (Günther)

Abstract

The peripheral blood cells of Acanthopagrus australis include erythrocytes, elongated and rounded thrombocytes, lymphocytes, neutrophils and monocytes. The ultrastructure of lymphocytes, neutrophils and monocytes is described. Type 1 cells with characteristic elongated, eosinophilic granules and type 2 cells with rounded, apparently labile granules are common in the gill filaments and do not increase or decrease significantly in abundance in ectoparasiteinfested gill filaments. Extravascular neutrophils tend to be more evident in pathologic tissue. Lymphocytes and the macrophage-like type 3 cells are the most abundant infiltrating cells in pathologic gill tissue. Apart from ultrastructural and histochemical data, granule length and width are sufficient criteria to distinguish between the neutrophil, type 1, type 2 and type 3 cells.

Ultrastructural observations on the phagocytic behavior of winter flounder Pleuronectes americanus peritoneal neutrophils and macrophages in vivo

“…12), they were morphologically similar to the scanning electron microscopic images of bacteria-laden neutrophils of humans after the in vitro uptake of large numbers of Eschenchia col] (MacRae et al 1980). The striate or filamentous-appearing granules we observed in winter flounder neutrophils were similar in shape and substructure to those observed in the heterophils of plaice (Ferguson 1976) and other teleost species (Cannon et al 1980b, Bodammer 1986, Hine et al 1986, Roubal 1986, Suzuki 1986, Hine & Wain 1988, Doggett & Harris 1989, Fujimaka & Isoda 1990. As mentioned earlier, because of th.e orientation of most of the neutrophil granules our images failed to reveal their microtubular nature most of the time (Figs.…”

Blood cells of the gilthead seabream (Sparus aurata L.): Light and electron microscopic studies

“…The first, probably corresponding to azurophilic granules, were filled by a uniform, granular, medium electron-dense material similar to those found in heterophils of Dicentrarchus labrax (Meseguer et al, 1990) and in type I granulocytes of Carassius uuratus (Davies and Haynes, 1975). Nevertheless, no nucleoids, needles or dense bodies like those observed in Acanthopagrus australis (Roubal, 1986) and Carassius auratus (Fujimaki and Isoda, 1990) were found. The second type of granule was characterized by a highly electron-dense content with scattered dense granules and an eccentric, rounded or elongated, laminar body.…”

Stromal Cells, Macrophages and Lymphoid Cells in the Head-kidney of Sea Bass (Dicentrarchus labrax L.). An Ultrastructural Study.

“…These may be two different functional stages of the same cell type (ELLIS, 1977), or two separate cell populations with different functional properties (PARROT and DESOUSA, 1971). Nevertheless, large and small lymphocytes have not been distinguished in some teleost species, with only a large lymphocyte type being recorded (WILLIAMS and WARNER, 1976;ELARIFI, 1982;ROUBAL, 1986). In the sea bass head-kidney we have identified both small and large lymphocytes according to the results of a light microscopical study previously carried out (ROMESTAND and TRILLES, 1984).…”