Blood and Synovial Microparticles as Revealed by Atomic Force and Scanning Electron Microscope

Junkar, Ita; Šuštar, Vid; Frank, Mojca; Janša, Vid; Zavec, Apolonija Bedina; Rozman, B; Mozetič, Miran; Hägerstrand, Henry; Kralj‐Iglič, Veronika

doi:10.2174/1876894600901010050

Cited by 22 publications

(18 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…17,18 Information on the presence of tumors could be obtained from cell-derived nanovesicles which are expected to be found in body fluids, such as blood, synovial fluid, ascites, pleural fluid, and cerebrospinal fluid. 19,20 For example, it was recently demonstrated that tumors of various origin 21 and various clinical outcomes 22 can be classified by their micro RNA (miRNA) profiles. miRNAs are short (about 18-25 nucleotides long) noncoding RNAs, including small interfering RNA, ribosomal RNA, transfer RNA, and small nuclear RNA.…”

Section: Introductionmentioning

confidence: 99%

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Kralj‐Iglič¹

2012

IJN

Self Cite

View full text Add to dashboard Cite

Abstract:Membranous nanostructures, such as nanovesicles and nanotubules, are an important pool of biological membranes. Recent results indicate that they constitute cell-cell communication systems and that cancer development is influenced by these systems. Nanovesicles that are pinched off from cancer cells can move within the circulation and interact with distant cells. It has been suggested and indicated by experimental evidence that nanovesicles can induce metastases from the primary tumor in this way. Therefore, it is of importance to understand better the mechanisms of membrane budding and vesiculation. Here, a theoretical description is presented concerning consistently related lateral membrane composition, orientational ordering of membrane constituents, and a stable shape of nanovesicles and nanotubules. It is shown that the character of stable nanostructures reflects the composition of the membrane and the intrinsic shape of its constituents. An extension of the fluid mosaic model of biological membranes is suggested by taking into account curvature-mediated orientational ordering of the membrane constituents on strongly anisotropically curved regions. Based on experimental data for artificial membranes, a possible antimetastatic effect of plasma constituents via mediation of attractive interaction between membranous structures is suggested. This mediated attractive interaction hypothetically suppresses nanovesiculation by causing adhesion of buds to the mother membrane and preventing them from being pinched off from the membrane.

show abstract

Section: Introductionmentioning

confidence: 99%

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Kralj‐Iglič¹

2012

IJN

Self Cite

View full text Add to dashboard Cite

show abstract

“…Vesiculation was studied in cancer cells [9][10][11][12][13] and was shown in vivo to be connected to the impact of cancer-associated coagulopathies. [14][15][16][17] Furthermore, nanoparticles have also been found in isolates from other body fluids, such as synovial fluid of inflamed joints, 18 pleural fluid, 19 ascites, 19 saliva, 20,21 and urine.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process

Šuštar¹,

Bedina-Zavec²,

Stukelj³

et al. 2011

IJN

Self Cite

View full text Add to dashboard Cite

Background: Shedding of nanoparticles from the cell membrane is a common process in all cells. These nanoparticles are present in body fluids and can be harvested by isolation. To collect circulating nanoparticles from blood, a standard procedure consisting of repeated centrifugation and washing is applied to the blood samples. Nanoparticles can also be shed from blood cells during the isolation process, so it is unclear whether nanoparticles found in the isolated material are present in blood at sampling or if are they created from the blood cells during the isolation process. We addressed this question by determination of the morphology and identity of nanoparticles harvested from blood. Methods: The isolates were visualized by scanning electron microscopy, analyzed by flow cytometry, and nanoparticle shapes were determined theoretically. Results: The average size of nanoparticles was about 300 nm, and numerous residual blood cells were found in the isolates. The shapes of nanoparticles corresponded to the theoretical shapes obtained by minimization of the membrane free energy, indicating that these nanoparticles can be identified as vesicles. The concentration and size of nanoparticles in blood isolates was sensitive to the temperature during isolation. We demonstrated that at lower temperatures, the nanoparticle concentration was higher, while the nanoparticles were on average smaller. Conclusion:These results indicate that a large pool of nanoparticles is produced after blood sampling. The shapes of deformed blood cells found in the isolates indicate how fragmentation of blood cells may take place. The results show that the contents of isolates reflect the properties of blood cells and their interaction with the surrounding solution (rather than representing only nanoparticles present in blood at sampling) which differ in different diseases and may therefore present a relevant clinical parameter.

show abstract

“…Visualization of the isolates obtained by this protocole by the scanning electron microscope and by atomic force microscope (Junkar et al, 2009) revealed shapes that are characteristic for particles without internal structure (such as discocytes, dumbbell shapes and "starfish" shapes (Seifert, 1997) that correspond to the minima of the membrane free energy at different ratios between membrane area and enclosed volume …”

Section: Accepted Manuscriptmentioning

confidence: 99%

A study of extracellular vesicle concentration in active diabetic Charcot neuroarthropathy

Schara

Štukelj

Krek

et al. 2017

European Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Blood and Synovial Microparticles as Revealed by Atomic Force and Scanning Electron Microscope

Cited by 22 publications

References 50 publications

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Stability of membranous nanostructures: a possible key mechanism in cancer progression

Nanoparticles isolated from blood: a reflection of vesiculability of blood cells during the isolation process

A study of extracellular vesicle concentration in active diabetic Charcot neuroarthropathy

Contact Info

Product

Resources

About