Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus allergens

Gernez, Yaël; Dunn, Colleen; Everson, C.; Mitsunaga, Erin M.; Gudiputi, Lakshmi; Krasinska, Karolina; Davies, Zoe; Herzenberg, Leonore A.; Tirouvanziam, Rabindra; Moss, Richard B.

doi:10.1016/j.jcf.2012.04.008

Cited by 39 publications

(63 citation statements)

References 35 publications

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“…[15][16][17] The basophil activation test (BAT) is a novel technique that measures upregulation of CD203c upon stimulation with the specific allergen to which an individual is sensitized. 18 CD203c is an ectonucleotide pyrophosphatase/phosphodiesterase expressed on the surface of basophils, which are important effector cells in type II immune responses. 18,19 CD203c can be rapidly measured by flow cytometry and has been proposed as a diagnostic tool in atopic disease, including peanut, drug and wasp venom allergy.…”

Section: Abbreviationsmentioning

confidence: 99%

“…18 CD203c is an ectonucleotide pyrophosphatase/phosphodiesterase expressed on the surface of basophils, which are important effector cells in type II immune responses. 18,19 CD203c can be rapidly measured by flow cytometry and has been proposed as a diagnostic tool in atopic disease, including peanut, drug and wasp venom allergy. [20][21][22] It has been shown that basophils are primed and hyper-responsive to Af allergen stimulation in CF-ABPA.…”

Section: Abbreviationsmentioning

confidence: 99%

“…[20][21][22] It has been shown that basophils are primed and hyper-responsive to Af allergen stimulation in CF-ABPA. 18 In this current study, BAT to Af was employed to identify Af sensitization in a CF cohort and it was correlated with key CF clinical measurements.…”

Section: Abbreviationsmentioning

confidence: 99%

“…18,25,26 Briefly, venous blood was collected in S-Monovette ® ethylenediaminetetraacetic acid (EDTA) blood tubes (Sarstedt, Germany) and centrifuged at 400g for 10 min at 4 °C. The supernatant was further centrifuged at 3000g for 10 min at 4 °C and 97 µl of platelet-free plasma was added to 100 µl of erythrocyte/leukocyte pellet and incubated for 30 s at 37 °C.…”

Section: Basophil Activation Testmentioning

confidence: 99%

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The basophil surface marker CD203c identifies Aspergillus species sensitization in patients with cystic fibrosis

Mirković

Lavelle

Azim

et al. 2016

Journal of Allergy and Clinical Immunology

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Section: Abbreviationsmentioning

confidence: 99%

Section: Abbreviationsmentioning

confidence: 99%

Section: Abbreviationsmentioning

confidence: 99%

Section: Basophil Activation Testmentioning

confidence: 99%

See 2 more Smart Citations

The basophil surface marker CD203c identifies Aspergillus species sensitization in patients with cystic fibrosis

Mirković

Lavelle

Azim

et al. 2016

Journal of Allergy and Clinical Immunology

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“…Thus, subsets of cells identified by flow cytometry are frequently compared to find such differences. Specifically, comparisons between a disease sample and control, different genetically modified organisms, or samples that have undergone stimulations provide fundamental information [20,21,22]. Therefore, it is important to have appropriate methods to characterize these differences in a quantitative and useful way.…”

Section: Discussionmentioning

confidence: 99%

Dynamic quantification of antigen molecules with flow cytometry

Moskalensky

Chernyshev

Yurkin

et al. 2015

Journal of Immunological Methods

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Traditional methods for estimating the number of expressed molecules, based on the detection of target antigens bound with fluorescently labeled antibodies, assume that the antigen-antibody reaction reaches equilibrium. A calibration procedure is used to convert the intensity of the fluorescence signal to the number of target molecules. Along with the different limitations of every calibration system, this substantially limits the applicability of the traditional approaches especially in the case of low affinity antibodies.We address this problem here with studies in which we demonstrate a new approach to the antigen molecule quantification problem. Instead of using a static calibration system, we analyzed mean fluorescence values over time by flow cytometry during antibody-antigen binding. Experimental data obtained with an LSRII cytometer were fitted by a diffusion-reaction mathematical model using the Levenberg-Marquardt nonlinear least squares curve-fitting algorithm in order to obtain the number of target antigen molecules per cell. Results were compared with the Quanti-BRITE calibration system. We conclude that, instead of using experiment-specific calibration, the value of the binding rate constant for each particular antibody-antigen reaction can be used to quantify antigen molecules with flow cytometry. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. HHS Public Access Author Manuscript Author ManuscriptAuthor ManuscriptAuthor Manuscript fluorescent label, medium viscosity and temperature). This approach is independent of specially prepared calibration beads, antibody reagents and the specific dye and can be applied to both low and high affinity antibodies, under both saturating and non-saturating binding conditions. The method was demonstrated on a human blood sample dataset investigating CD8α antigen on T cells in stable binding conditions.

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The myriad challenges of respiratory fungal infection in cystic fibrosis

Tracy

Moss

2018

Pediatric Pulmonology

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Fungal infection in cystic fibrosis (CF) is a recognized challenge, with many areas requiring further investigation. Consensus definitions exist for allergic bronchopulmonary aspergillus in CF, but the full scope of clinically relevant non-allergic fungal disease in CF-asymptomatic colonization, transient or chronic infection localized to endobronchial mucus plugs or airway tissue, and invasive disease-is yet to be clearly defined. Recent advances in mycological culture and non-culture identification have expanded the list of both potential pathogens and community commensals in the lower respiratory tract. Here we aim to outline the current understanding of fungal presence in the CF respiratory tract, risk factors for acquiring fungi, host-pathogen interactions that influence the role of fungi from bystander to pathogen, advances in the diagnostic approaches to isolating and identifying fungi in CF respiratory samples, challenges of classifying clinical phenotypes of CF patients with fungi, and current treatment approaches. Development and validation of biomarkers characteristic of different fungal clinical phenotypes, and controlled trials of antifungal agents in well-characterized target populations, remain central challenges to surmount and goals to be achieved.

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Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus allergens

Abstract: In CF-ABPA, blood basophils are primed and hyperresponsive to Af allergen stimulation.

Cited by 39 publications

References 35 publications

The basophil surface marker CD203c identifies Aspergillus species sensitization in patients with cystic fibrosis

The basophil surface marker CD203c identifies Aspergillus species sensitization in patients with cystic fibrosis

Dynamic quantification of antigen molecules with flow cytometry

The myriad challenges of respiratory fungal infection in cystic fibrosis

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