Blood bioactive sphingolipids in patients with advanced serous epithelial ovarian cancer – mass spectrometry analysis

Knapp, Paweł; Bodnar, Lubomir; Błachnio-Zabielska, Agnieszka; Reszeć, Joanna; Świderska, Magdalena; Chabowski, Adrian

doi:10.5114/aoms.2018.76996

Cited by 3 publications

(4 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the purpose of our previous research was to analyze how the levels of specific sphingolipids (ceramides, sphingosine-1-phosphate, sphingosine, and sphinganine) are changed in serum, red blood cells, and platelets of patients diagnosed with advanced serous ovarian cancer (ASOC) [39]. Our observations of a notably higher plasma level of C16-Cer, C18:1-Cer, and C18-Cer in the advanced ovarian cancer group were compared with the results of the control one.…”

Section: Sphingolipids As Diagnostic Biomarkersmentioning

confidence: 99%

“…C16-Cer > 315.98 ng/100 ml; C18:1-Cer > 4.81 ng/100 ml and C18-Cer > 113.26 ng/100 ml; RBC C18:1-Cer > 0.1 ng/100 ml. Additionally, C16, C18.1, and C18 plasma levels corresponded with the progression of the tumor: the FIGO staging and grading [6, 39].…”

Section: Sphingolipids As Diagnostic Biomarkersmentioning

confidence: 99%

“…A unique switch into the carcinogenesis pathways recognized as the elevation of particular CER concentrations across cut points seems helpful in taking further steps in the diagnosis. The estimated levels of plasma C16-Cer, C18:1-Cer, and C18-Cer could be a crucial factor in making the decision regarding an adequate surgery or procedure [6, 39], such as a biopsy, which is frequently performed when the tumor is in the development stage III/IV. Furthermore, our previous research demonstrated different levels of the total investigated CER in RBC, platelets, and plasma of patients with ASOC.…”

Section: Sphingolipids As Diagnostic Biomarkersmentioning

confidence: 99%

“…This corresponds to the findings of Blachnio-Zabielska et al who documented similar stable levels of those sphingolipids in RBC and PLT with a reduction in the levels of CER, SPH, and SPA in contrast to S1P in the liver of rats treated with myriocin [33]. Some of the sphingolipids are unaltered in the blood cells of patients who suffer from ASOC due to the high grade of malignancy of this tumor which produces S1P responsible for cell proliferation, neoangiogenesis, and antiapoptotic processes [6, 39]. Presently, an increasing number of studies are dedicated to markers in the advanced stages of ovarian cancer [40–45].…”

Section: Sphingolipids As Diagnostic Biomarkersmentioning

confidence: 99%

See 3 more Smart Citations

Unique Roles of Sphingolipids in Selected Malignant and Nonmalignant Lesions of Female Reproductive System

Knapp

Chomicz

Świderska

et al. 2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Cancer develops as a result of the loss of self-control mechanisms by a cell; it gains the ability to induce angiogenesis, becomes immortal and resistant to cell death, stops responding to growth suppressor signals, and becomes capable of invasion and metastasis. Sphingolipids—a family of membrane lipids—are known to play important roles in the regulation of cell proliferation, the response to chemotherapeutic agents, and/or prevention of cancer. Despite the underlying functions of sphingolipids in cancer biology, their metabolism in different malignant tumors is poorly investigated. Some studies showed marked differences in ceramide content between the tumor and the respective healthy tissue. Interestingly, the level of this sphingolipid could be either low or elevated, suggesting that the alterations in ceramide metabolism in cancer tissue may depend on the biology of the tumor. These processes are indeed related to the type of cancer, its stage, and histology status. In this paper we present the unique roles of bioactive sphingolipid derivative in selected gynecologic malignant and nonmalignant lesions.

show abstract

Section: Sphingolipids As Diagnostic Biomarkersmentioning

confidence: 99%

Section: Sphingolipids As Diagnostic Biomarkersmentioning

confidence: 99%

Section: Sphingolipids As Diagnostic Biomarkersmentioning

confidence: 99%

Section: Sphingolipids As Diagnostic Biomarkersmentioning

confidence: 99%

See 2 more Smart Citations

Unique Roles of Sphingolipids in Selected Malignant and Nonmalignant Lesions of Female Reproductive System

Knapp

Chomicz

Świderska

et al. 2019

BioMed Research International

Self Cite

View full text Add to dashboard Cite

show abstract

The Lard Works in Mysterious Ways: Ceramides in Nutrition-Linked Chronic Disease

et al. 2022

View full text Add to dashboard Cite

Diet influences onset, progression, and severity of several chronic diseases, including heart failure, diabetes, steatohepatitis, and a subset of cancers. The prevalence and clinical burden of these obesity-linked diseases has risen over the past two decades. These metabolic disorders are driven by ectopic lipid deposition in tissues not suited for fat storage, leading to lipotoxic disruption of cell function and survival. Sphingolipids such as ceramides are among the most deleterious and bioactive metabolites that accrue, as they participate in selective insulin resistance, dyslipidemia, oxidative stress and apoptosis. This review discusses our current understanding of biochemical pathways controlling ceramide synthesis, production and action; influences of diet on ceramide levels; application of circulating ceramides as clinical biomarkers of metabolic disease; and molecular mechanisms linking ceramides to altered metabolism and survival of cells. Development of nutritional or pharmacological strategies to lower ceramides could have therapeutic value in a wide range of prevalent diseases. Expected final online publication date for the Annual Review of Nutrition, Volume 42 is August 2022. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

show abstract

An Update on Sphingolipidomics: Is Something Still Missing? Some Considerations on the Analysis of Complex Sphingolipids and Free-Sphingoid Bases in Plasma and Red Blood Cells

et al. 2022

View full text Add to dashboard Cite

The main concerns in targeted “sphingolipidomics” are the extraction and proper handling of biological samples to avoid interferences and achieve a quantitative yield well representing all the sphingolipids in the matrix. Our work aimed to compare different pre-analytical procedures and to evaluate a derivatization step for sphingoid bases quantification, to avoid interferences and improve sensitivity. We tested four protocols for the extraction of sphingolipids from human plasma, at different temperatures and durations, and two derivatization procedures for the conversion of sphingoid bases into phenylthiourea derivatives. Different columns and LC-MS/MS chromatographic conditions were also tested. The protocol that worked better for sphingolipids analysis involved a single-phase extraction in methanol/chloroform mixture (2:1, v/v) for 1 h at 38 °C, followed by a 2 h alkaline methanolysis at 38 °C, for the suppression of phospholipids signals. The derivatization of sphingoid bases promotes the sensibility of non-phosphorylated species but we proved that it is not superior to a careful choice of the appropriate column and a full-length elution gradient. Our procedure was eventually validated by analyzing plasma and erythrocyte samples of 20 volunteers. While both extraction and methanolysis are pivotal steps, our final consideration is to analyze sphingolipids and sphingoid bases under different chromatographic conditions, minding the interferences.

show abstract

Blood bioactive sphingolipids in patients with advanced serous epithelial ovarian cancer – mass spectrometry analysis

Cited by 3 publications

References 38 publications

Unique Roles of Sphingolipids in Selected Malignant and Nonmalignant Lesions of Female Reproductive System

Unique Roles of Sphingolipids in Selected Malignant and Nonmalignant Lesions of Female Reproductive System

The Lard Works in Mysterious Ways: Ceramides in Nutrition-Linked Chronic Disease

An Update on Sphingolipidomics: Is Something Still Missing? Some Considerations on the Analysis of Complex Sphingolipids and Free-Sphingoid Bases in Plasma and Red Blood Cells

Contact Info

Product

Resources

About