Blood biomarkers of uveal melanoma metastasis

Triozzi, Pierre L.; Singh, Arun D.

doi:10.1136/bjo.2010.193896

Cited by 8 publications

(14 citation statements)

References 22 publications

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“…An alternative substrate that could closely recapitulate the BrM may be generated by electrospinning techniques to produce a thin scaffold on which RPE cells can be cultured in-vitro ( Treharne et al, 2011 ). These advanced BrM mimics could also act as a prosthetic support on to which heathy RPE cells could be transplanted in cell-replacement therapy ( Treharne et al, 2011 , Heller and Martin, 2014 , Thomson et al, 2011 ). Such a prosthetic support by definition must fulfil key criteria of native BrM and be capable of robustly supporting the attachment, spread and differentiation of cells to become a specialised RPE monolayer.…”

Section: Introductionmentioning

confidence: 99%

“…1 B–C) ( Treharne et al, 2012 ). The electrospun scaffold was specifically developed with inert materials, such that it could act as a potential substrate for future cell-replacement treatments ( Treharne et al, 2011 , Thomson et al, 2011 ). Key biophysical characteristics of our first-generation electrospun scaffold have since been modified to create an improved second-generation electrospun substrate which mimics the BrM more faithfully ( Ward et al, 2014 , Lotery et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Ex-vivo models of the Retinal Pigment Epithelium (RPE) in long-term culture faithfully recapitulate key structural and physiological features of native RPE

et al. 2017

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ex-vivo models of the Retinal Pigment Epithelium (RPE) in long-term culture faithfully recapitulate key structural and physiological features of native RPE

et al. 2017

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“…5 In the global context, UM is a rare tumor, with an incidence of 4-7 per million per year. [8][9][10] Gene mutation is regarded as the most significant trigger of melanoma tumorigenesis. Uncontrolled metastasis is fatal, and people who are not treated in time survive only 2-6 months.…”

Section: Introductionmentioning

confidence: 99%

The oncolytic virus H101 combined with GNAQ siRNA‐mediated knockdown reduces uveal melanoma cell viability

Qiu

et al. 2018

J of Cellular Biochemistry

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Background Uveal melanoma (UM) is a severe human malignancy with a high mortality rate, as well as high metastasis and recurrence potential. The active mutation of G protein subunit alpha q (GNAQ) or G protein subunit alpha 11 (GNA11) is a major trigger for UM. Oncolytic adenovirus H101 (H101) is the first oncolytic virus approved for clinical applications in cancer therapy by the China Food and Drug Administration. We investigated whether combining H101 with the downregulation of GNAQ expression would act synergistically in UM therapy. Methods Three UM cell lines OMM2.3 and 92.1, harboring GNAQ mutation, and OCM1, harboring B‐Raf proto‐oncogene mutation, were chosen for our research. The cellular toxicity of adenoviral infection and the cell growth rate were measured with a Cell Counting Kit‐8. Western blot analysis was used to detect GNAQ, p‐MEK1/2, YAP, and p‐YAP expression. The apoptosis and cell‐cycle distribution of cells were evaluated with annexin‐V and propidium iodide staining. Results Our results revealed that OMM2.3 and 92.1 cells were more sensitive to H101 infection than OCM1 cells. GNAQ expression was markedly reduced by small interfering RNA, siGNAQ. Combined treatment of siGNAQ and H101 inhibited the proliferation and activated the apoptosis of OMM2.3 and 92.1 cells by blocking the phosphorylation of MEK1/2 and increasing the phosphorylation of YAP. Conclusions In summary, a therapy combining H101 and siGNAQ is feasible, with potential utility as a novel targeted molecular therapy for UM, especially those carrying a GNAQ mutation.

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“…25,26 Melanoma inhibitory activity, 27,28 osteopontin, 29 S100 beta, 29,30 hepatocyte growth factor, epidermal growth factor and insulin-like growth factor have been associated with metastatic uveal melanoma and may be useful as a screening test in the future. 31…”

Section: Levels Of Circulating Tumour Cells and Levels Of Tyrosinase Ormentioning

confidence: 99%

Imaging Modalities for Uveal Melanoma

Mirchia¹,

Turell²,

Singh³

2012

European Ophthalmic Review

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Purpose: To review the spectrum of imaging modalities commonly used in clinical practice for the diagnosis and management of uveal melanoma. Materials and Methods: Review of literature. Results: Various forms of ophthalmic imaging are useful in the characterisation and treatment planning of uveal melanoma. These include digital photography, ultrasonography, angiography and optical coherence tomography. Digital photography is useful for documenting the clinical findings for future comparison and response to treatment. Ultrasonography provides information on tumour biometry, local extension and tumour composition (internal reflectivity). Angiography can be used to assess the degree of tumour vascularity. Newer forms of imaging, including optical coherence tomography (OCT), have excellent resolution and provide detail on surface features. Conclusion: The accuracy of clinical diagnosis of uveal melanoma exceeds 99 % when using currently available imaging modalities. There are advantages and disadvantages to each modality depending on tumour location, size, surface characteristics and internal structure. Ancillary techniques such as computed tomography, magnetic resonance imaging and positron emission tomography may be useful in atypical lesions or in the surveillance for metastatic disease.Uveal melanoma is the most common primary intraocular malignancy in adults and comprises approximately 85 % of all ocular melanomas. 1

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Blood biomarkers of uveal melanoma metastasis

Cited by 8 publications

References 22 publications

Ex-vivo models of the Retinal Pigment Epithelium (RPE) in long-term culture faithfully recapitulate key structural and physiological features of native RPE

Ex-vivo models of the Retinal Pigment Epithelium (RPE) in long-term culture faithfully recapitulate key structural and physiological features of native RPE

The oncolytic virus H101 combined with GNAQ siRNA‐mediated knockdown reduces uveal melanoma cell viability

Imaging Modalities for Uveal Melanoma

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