Blood-brain barrier characteristic enzymatic properties in cultured brain capillary endothelial cells

Meyer, Jörg; Mischeck, Uwe; Veyhl, Maike; Henzel, Karin; Galla, Hans‐Joachim

doi:10.1016/0006-8993(90)91425-g

Cited by 106 publications

(49 citation statements)

References 20 publications

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“…Coculturing systems of astrocytes and noncerebral endothelial cells partially mimic BBB, since G G T expression is triggered in such systems (Gebhart and Goldstein, 1988). Therefore, the absence of astrocytes may lead to the deficit in G G T expression which has been observed by measuring the G G T activity of cultured BMEC (Mischeck et al, 1989;Meyer et al, 1990). Consistent with these data we could demonstrate that cultured BMEC show a lower G G T immunoffuorescence than non-proliferating clusters of BMEC.…”

Section: Discussionsupporting

confidence: 87%

Pericytes of the brain microvasculature express γ‐glutamyl transpeptidase

Frey

Meckelein

Weiler-Güttler

et al. 1991

European Journal of Biochemistry

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The expression of y-glutamyl transpeptidase (GGT) is a specific property of the brain capillary endothelium that constitutes the blood-brain barrier. We report here the detection of GGT, not only in endothelial cells, but also in pericytes, demonstrating that a brain capillary-specific pericyte population exists. We raised antibodies to GGT using a porcine brain microvessel GGT -protein-A (staphylococcal protein A) fusion protein as antigen which was expressed in Escherichia coli. The immunohistochemical analysis of the subcapillary distribution of GGT in porcine brain cortex and cerebellum sections by both light and electron microscopy revealed the expression of GGT in the capillary-adjacent pericytes in addition to the GGT-positive endothelial layer. We confirmed these data for cultured porcine brain microvascular endothelial cells and pericytes. GGT immunofluorescence could be detected in both cell types in culture. Endothelial cells exhibited a weak staining, whereas pericytes were strongly positive for GGT. Due to the high phagocytotic activity of pericytes and their location on the abluminal surface of the microvessels, we propose a possible protective or detoxifying function of GGT in cerebrovascular pericytes.

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Section: Discussionsupporting

confidence: 87%

Pericytes of the brain microvasculature express γ‐glutamyl transpeptidase

Frey

Meckelein

Weiler-Güttler

et al. 1991

European Journal of Biochemistry

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“…Cells were grown in Earle's medium containing 20% fetal calf serum, penicillin G, streptomycin and amphotericin B at 37°C in an incubator containing 95% air and 5% CO2. Alkaline phosphatase and y-glutamyltranspeptidase activity were used as marker enzymes for brain microvessel endothelial cells (Betz et al 1980;Mischek et al 1989;Meyer, Mischek, Veyhl, Henzel & Galla, 1990). Single cells were isolated from the primary culture (up to 14 days) by trypsinization (0250%).…”

Section: Introductionmentioning

confidence: 99%

Stretch‐activated non‐selective cation channels in the antiluminal membrane of porcine cerebral capillaries.

Popp

Hoyer

Meyer

et al. 1992

The Journal of Physiology

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SUMMARY1. Single stretch-activated (SA) channels have been studied in isolated brain capillary endothelial cells as well as in the antiluminal membrane of intact porcine cerebral capillaries using the patch-clamp recording technique.2. The SA channels were found to be cation selective and permeable to Nat, K+, Ba2+ and Ca2 . 3. With monovalent cations in the patch pipette, the channels showed inward rectification in cell-attached patches with a single-channel conductance of 37 pS at negative and 24 pS at positive clamp potentials.4. With either 70 mM-Ca2+ or Ba2+ in the patch pipette, the current-voltage relation was linear with slope conductances of 16 and 19 pS, respectively.5. Mean channel open probability increased with increasing pressure and with depolarizing clamp potentials.6. Cell swelling induced by hypotonic shock activated the SA channels in cellattached experiments.7. The SA channel may be involved in cell volume or blood flow regulation. The contribution of these channels to the regulation of cerebrospinal salt and water content, especially in brain oedema, is discussed.

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“…Topographic membrane localization of these transporters is indicative of the polarity of the endothelial functions and differentiation that sets apart the BBB endothelium from other vascular beds. The BBB endothelial cytoplasm is richly endowed with enzymes(18) including adenosine triphosphatase, acid and alkaline phosphatases, Na + /K + /ATPase, monoamine oxidase, cytochrome p450s and various dehydrogenases (19)(20)(21)(22). The BBB ECs are also characterized by very high density of mitochondria denoting high metabolic activity (23) to support all the specialized cellular activities bestowed upon these highly specialized cells.…”

Section: Structural and Functional Complexity Of Blood Brain Barrier mentioning

confidence: 99%

Role and Function of Dehydrogenases in CNS and Blood-Brain Barrier Pathophysiology

Naik¹,

Prasad²,

Cucullo³

2012

Dehydrogenases

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Blood-brain barrier characteristic enzymatic properties in cultured brain capillary endothelial cells

Cited by 106 publications

References 20 publications

Pericytes of the brain microvasculature express γ‐glutamyl transpeptidase

Pericytes of the brain microvasculature express γ‐glutamyl transpeptidase

Stretch‐activated non‐selective cation channels in the antiluminal membrane of porcine cerebral capillaries.

Role and Function of Dehydrogenases in CNS and Blood-Brain Barrier Pathophysiology

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