Blood cell capture in a sawtooth dielectrophoretic microchannel

Jones, Paul V.; Staton, Sarah J. R.; Hayes, Mark A.

doi:10.1007/s00216-011-5284-9

Cited by 49 publications

(54 citation statements)

References 36 publications

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“…Microfluidic devices have the potential to improve each of these figures of merit and provide increased portability. A wide range of bioparticles can be addressed, including animal cells 1 , organelles 2 , proteins 3, 4 , DNA 5–7 and bacteria. 8–11 …”

Section: Introductionmentioning

confidence: 99%

Refinement of insulator-based dielectrophoresis

Crowther

Hayes

2017

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The ability to separate analytes with increasingly similar properties drives the field of separation science. One way to achieve such separations is using trapping and streaming dielectrophoresis (DEP), which directly exploits the subtle differences in the electrophysical properties of analytes. The non-uniform fields necessary for DEP can be formed using various insulator shapes in microchannels. Current insulator shapes include triangles, diamonds, circles, and rectangles. However, all of these insulators pose problems for trapping, streaming, and sorting (deflection) as the induced fields/gradients are not behaviorally consistent across the lateral dimension. This leads to analytes experiencing different forces depending on their pathline in the microchannel and result in low resolution separations. Based on an iterative process that explored approximately 40 different insulator shapes, a design was chosen that indicated improved particle streamlines, better trapping efficiency, and consistent electrical environments across the lateral dimension. The design was assessed by simulations where the electric field, gradient of the electric field squared, and the ratio of the two were plotted. The improved design includes a unique new multi-length scale element. The multi-length scale structure streamlines the analyte(s) and improves homogeneity in the lateral dimension, while still achieving high gradients necessary for analyte separation using DEP. The design is calculated to keep analytes on the centerline which should improve resolution, and eliminate extraneous trapping zones. Behaviors consistent with the features of the simulations were observed in proof of principle experiments using representative test probes.

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Section: Introductionmentioning

confidence: 99%

Refinement of insulator-based dielectrophoresis

Crowther

Hayes

2017

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“…Other authors either required precise regulation of four voltages to separate white blood cells [21] or high voltage (200-700 V) was used to separate blood cells from diluted blood in a sawtooth-shape channel [23]. Also, in some cases particles were added after the medium filled the device, whereas in the current study both particles (blood cells) and medium (heparinized plasma) are simultaneously introduced into the channel.…”

Section: Discussionmentioning

confidence: 95%

“…Since then, DC-iDEP devices have been applied to different cells and configurations such as E. coli bacteria [20], white blood cells [21], RBCs [22], or blood cell separation in a sawtooth microchannel [23] (also see the review of DC-iDEP studies [24]). The aforementioned works provide valuable insight into DC-iDEP phenomena.…”

Section: Introductionmentioning

confidence: 99%

Hydrodynamic and direct-current insulator-based dielectrophoresis (H-DC-iDEP) microfluidic blood plasma separation

et al. 2015

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Evaluation and diagnosis of blood alterations is a common request for clinical laboratories, requiring a complex technological approach and dedication of health resources. In this paper, we present a microfluidic device that owing to a novel combination of hydrodynamic and dielectrophoretic techniques can separate plasma from fresh blood in a microfluidic channel and for the first time allows optical real-time monitoring of the components of plasma without pre- or post-processing. The microchannel is based on a set of dead-end branches at each side and is initially filled using capillary forces with a 2-μL droplet of fresh blood. During this process, stagnation zones are generated at the dead-end branches and some red blood cells (RBCs) are trapped there. An electric field is then applied and dielectrophoretic trapping of RBCs is used to prevent more RBCs entering into the channel, which works like a sieve. Besides, an electroosmotic flow is generated to sweep the rest of the RBCs from the central part of the channel. Consequently, an RBC-free zone of plasma is formed in the middle of the channel, allowing real-time monitoring of the platelet behavior. To study the generation of stagnation zones and to ensure RBC trapping in the initial constrictions, two numerical models were solved. The proposed experimental design separates up to 0.1 μL blood plasma from a 2-μL fresh human blood droplet. In this study, a plasma purity of 99 % was achieved after 7 min, according to the measurements taken by image analysis. Graphical Abstract Schematics of a real-time plasma monitoring system based on a Hydrodynamic and direct-current insulator-based dielectrophoresis microfluidic channel.

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“…These devices are more appropriate for metal-sensitive organic samples. The iDEP microdevices including two separated electrodes in inlet and outlet have been successfully employed for different applications: separation and concentration of live and dead bacteria [13][14] separation of white blood cells [15], red blood cells (RBC) [16] or blood cell separation in a sawtooth microchannel [17] (see the review [18]). …”

Section: Introductionmentioning

confidence: 99%

A new approach to design an efficient micropost array for enhanced direct-current insulator-based dielectrophoretic trapping

et al. 2016

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Aquesta és una còpia de la versió author's final draft d'un article publicat a la revista Analytical and bioanalytical chemistry.La publicació final està disponible a Springer a través de http://dx.doi.org/10.1007/s00216-016-9629-2 This is a copy of the author 's final draft version of an article published in the journal Analytical and bioanalytical chemistry. AbstractDirect-current insulator-based dielectrophoresis (DC-iDEP) is a well-known technique that benefits from the electric field gradients generated by an array of insulating posts to separate or trap biological particles. In this work, we propose a novel figure of merit to find an efficient design of the post-array distribution in a microfluidic channel. To characterization can be used to reduce the required electric field to achieve effective particle trapping and, therefore, avoid the negative effects of Joule heating in cells or viable particles.

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Blood cell capture in a sawtooth dielectrophoretic microchannel

Cited by 49 publications

References 36 publications

Refinement of insulator-based dielectrophoresis

Refinement of insulator-based dielectrophoresis

Hydrodynamic and direct-current insulator-based dielectrophoresis (H-DC-iDEP) microfluidic blood plasma separation

A new approach to design an efficient micropost array for enhanced direct-current insulator-based dielectrophoretic trapping

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