Blood clot parameters: Thromboelastography and scanning electron microscopy in research and clinical practice

Pretorius, Etheresia; Swanepoel, Albe C.; DeVilliers, Sulette; Bester, Janette

doi:10.1016/j.thromres.2017.04.005

Cited by 46 publications

(55 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…the TEG results of the naïve WB, as well as the results after 24-hour exposure with the 4 different molecules. There is a significantly decreased R-value in the presence of LPS, suggesting that the LPS causes WB to clot faster [88]. This was also previously demonstrated with a lower LPS concentration.…”

Section: Confocal Microscopysupporting

confidence: 79%

See 1 more Smart Citation

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker^™stains

Pretorius

Pagé

Hendricks

et al. 2017

Preprint

Self Cite

View full text Add to dashboard Cite

In recent work, we discovered that the presence of highly substoichiometric amounts (10 -8 molar ratio) of lipopolysaccharide (LPS) from Gram-negative bacteria caused fibrinogen clotting to lead to the formation of an amyloid form of fibrin. We here show that the broadly equivalent lipoteichoic acids (LTAs) from two species of Gram-positive bacteria have similarly (if not more) potent effects. Using thioflavin T fluorescence to detect amyloid as before, the addition of low concentrations of free ferric ion is found to have similar effects. Luminescent conjugated oligothiophene dyes (LCOs), marketed under the trade name Amytracker TM , also stain classical amyloid structures. We here show that they too give very large fluorescence enhancements when clotting is initiated in the presence of the four amyloidogens (LPS, ferric ions and two LTA types). The staining patterns differ significantly as a function of both the amyloidogens and the dyes used to assess them, indicating clearly that the nature of the clots formed is different. This is also the case when clotting is measured viscometrically using thermoelastography. Overall, the data provide further evidence for an important role of bacterial cell wall products in the various coagulopathies that are observable in chronic, inflammatory diseases. The assays may have potential in both diagnostics and therapeutics.

show abstract

Section: Confocal Microscopysupporting

confidence: 79%

“…The samples were then placed in a Thromboelastograph 5000 Hemostasis Analyzer System for analysis. Seven parameters, as shown in Table 1, were studied [88][89][90]. Table 1: TEG clot parameters for whole blood and platelet poor plasma (taken from [88]).…”

Section: Clot Property Studiesmentioning

confidence: 99%

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker^™stains

Pretorius

Pagé

Hendricks

et al. 2017

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Results from WB TEG analysis showed clot properties due to both the cellular (platelets and RBC) components, as well as the plasma protein components (Pretorius et al , ). Whole blood TEG parameters are also affected by immune cell contributions.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, PPP clot results show only the effect of the plasma proteins, which mostly consists of fibrin(ogen). Seven parameters, as shown in Table , were studied (Bester et al , ; de Villiers et al , ; Pretorius et al , ).…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, PPP clot results show only the effect of the plasma proteins, which mostly consists of fibrin(ogen). Seven parameters, as shown in Table I, were studied de Villiers et al, 2015;Pretorius et al, 2017). Table II shows sample demographics and TEG results for both WB and PPP before and after exposure to IL12.…”

Section: Thromoelastography Of Whole Blood and Platelet Poor Plasmamentioning

confidence: 99%

See 1 more Smart Citation

Interleukin‐12 and its procoagulant effect on erythrocytes, platelets and fibrin(ogen): the lesser known side of inflammation

2017

Self Cite

View full text Add to dashboard Cite

SummaryInflammation, with its associated inflammatory molecules, is integral to most chronic diseases, including the various cardiovascular diseases. Interleukin 12 (IL12) is one of the inflammatory cytokines that is upregulated during inflammation; however, we know very little about its exact effect on red blood cells (RBCs), platelets and fibrin(ogen). IL12 is an important pleiotropic cytokine in early inflammatory responses and has potent immunomodulatory, antitumour and anti-infection activity. Here we investigate how low levels of circulating IL12, comparable to levels found during chronic inflammation, affect coagulation parameters, platelets and RBCs. We used thromboelastography, scanning electron microscopy, refractometery and wide-field microscopy. Our results show that IL12 caused hypercoagulation, platelet activation and spreading, as well as RBC agglutination. This phenomenon has far-reaching implications for treatment of the plethora of conditions where IL12 is upregulated, since it suggests aberrant haemorheology as agglutination affects blood flow. This information might be used in future to target the lowering of IL12 in inflammatory conditions, as well as address RBC agglutination.

show abstract

Personalized Cardiac Anesthesia

Piliego

Rizzo

Sebastiani

2020

Personalized Medicine in Anesthesia, Pain and Perioperative Medicine

View full text Add to dashboard Cite

Blood clot parameters: Thromboelastography and scanning electron microscopy in research and clinical practice

Cited by 46 publications

References 18 publications

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker^™stains

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker^™stains

Interleukin‐12 and its procoagulant effect on erythrocytes, platelets and fibrin(ogen): the lesser known side of inflammation

Personalized Cardiac Anesthesia

Contact Info

Product

Resources

About

Blood clot parameters: Thromboelastography and scanning electron microscopy in research and clinical practice

Cited by 46 publications

References 18 publications

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker™stains

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker™stains

Interleukin‐12 and its procoagulant effect on erythrocytes, platelets and fibrin(ogen): the lesser known side of inflammation

Personalized Cardiac Anesthesia

Contact Info

Product

Resources

About

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker^™stains

Both lipopolysaccharide and lipoteichoic acids potently induce anomalous fibrin amyloid formation: assessment with novel Amytracker^™stains