Blood coagulation and osmolar tolerance of erythrocytes in stroke-prone spontaneously hypertensive rats given rapeseed oil or soybean oil as the only dietary fat

Naito, Yukiko; Konishi, Chika; Ohara, Naoki

doi:10.1016/s0378-4274(00)00224-1

Cited by 29 publications

(25 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, erythrocytes need to be resistant to osmotic pressure to prevent haemolysis. Serum plant sterol and stanol concentrations in these rat studies (Naito et al, 2000;Ratnayake et al, 2000), however, were 40-80 times higher as compared to physiological concentrations in humans. The question therefore remains whether these findings can be extrapolated to humans consuming plant sterol or stanolenriched functional foods.…”

Section: Introductioncontrasting

confidence: 58%

“…In contrast, studies in the SHRSP rat model did show unfavourable effects on erythrocyte deformability (Ratnayake et al, 2000) and osmotic fragility (Naito et al, 2000(Naito et al, , 2003 after consumption of canola oil, which is abundant in plant sterols. A change in erythrocyte properties can be attributed to both a decrease in cholesterol levels of the red blood cell membrane as well as to an increase in plant sterol levels in the membrane (Naito et al, 2003).…”

Section: Discussionmentioning

confidence: 82%

“…Red blood cell deformability was, however, only decreased in the plant sterol group, whereas the lifespan was more shortened in the plant stanol-fed rats (Ratnayake et al, 2003). In the same rat model, osmotic fragility was also increased after canola oil consumption (Naito et al, 2000(Naito et al, , 2003. However, these effects were not found in hamsters fed plant stanol esters (Ebine et al, 2005).…”

Section: Discussionmentioning

confidence: 89%

“…We further found that serum concentrations of campesterol and sitosterol were increased in the plant sterol group, while red blood cell campesterol levels did not change. Animal studies (Naito et al, 2000;Ratnayake et al, 2003), as well as clinical features of sitosterolaemic patients, who are characterized by high plasma plant sterol concentrations (Bjorkhem and Boberg, 1994), have however suggested unfavourable effects of increased plant sterol concentrations on erythrocyte properties. In agreement with our results, however, Jenkins et al (2002) found no effect on osmotic fragility in hyperlipidaemic subjects of a combined intake of plant sterols, viscous fibres and vegetable proteins.…”

Section: Discussionmentioning

confidence: 99%

“…Red blood cells also incorporate plant sterols into their membranes (Bruckerdorfer et al, 1969), which may make erythrocytes less deformable and more fragile. For example, Naito et al (2000) showed increased osmotic fragility in stroke-prone spontaneously hypertensive (SHRSP) rats after canola oil consumption, which is rich in plant sterols. Ratnayake et al (2000) showed decreased red blood cell deformability in SHRSP rats after plant sterol consumption.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Plant sterol or stanol consumption does not affect erythrocyte osmotic fragility in patients on statin treatment

Jong

Mensink

2006

Eur J Clin Nutr

View full text Add to dashboard Cite

Objective: To assess the effects of plant sterol or stanol ester consumption on their incorporation into erythrocytes and their effects on osmotic fragility of red blood cells. Design: Double-blind, randomized, placebo-controlled intervention trial. Subjects and intervention: Forty-one subjects on stable statin treatment -who already have increased serum plant sterol and stanol concentrations -first received for 4 weeks a control margarine. For the next 16 weeks, subjects were randomly assigned to one of three possible interventions. Eleven subjects continued with control margarine, 15 subjects with plant sterol ester enriched and 15 subjects with plant stanol ester-enriched margarine. Daily plant sterol or stanol intake was 2.5 g. Erythrocyte haemolysis was measured spectrophotometrically at five different saline concentrations. Results: Despite significant (P ¼ 0.004) increases of, respectively, 42 and 59% in cholesterol-standardized serum sitosterol and campesterol concentrations in the plant sterol group as compared to the control group, campesterol levels in the red blood cells did not change (P ¼ 0.196). Osmotic fragility did not change significantly (P ¼ 0.757) in the plant sterol and plant stanol groups as compared to the control group. Conclusion: We conclude that plant sterol and stanol ester consumption for 16 weeks does not change osmotic fragility of erythrocytes in statin-treated patients.

show abstract

Section: Introductioncontrasting

confidence: 58%

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Plant sterol or stanol consumption does not affect erythrocyte osmotic fragility in patients on statin treatment

Jong

Mensink

2006

Eur J Clin Nutr

View full text Add to dashboard Cite

show abstract

Brassica napus

Lim¹

2012

Edible Medicinal and Non-Medicinal Plants

View full text Add to dashboard Cite

Comparative health effects of margarines fortified with plant sterols and stanols on a rat model for hemorrhagic stroke

Ratnayake

Plouffe

L’Abbé

et al. 2003

Lipids

View full text Add to dashboard Cite

There is increased acceptance of fortifying habitual foods with plant sterols and their saturated derivatives, stanols, at levels that are considered safe. These sterols and stanols are recognized as potentially effective dietary components for lowering plasma total and LDL cholesterol. Our previous studies have shown that daily consumption of plant sterols promotes strokes and shortens the life span of stroke-prone spontaneously hypertensive (SHRSP) rats. These studies question the safety of plant sterol additives. The present study was performed to determine whether a large intake of plant stanols would cause nutritional effects similar to those seen with plant sterols in SHRSP rats. Young SHRSP rats (aged 26-29 d) were fed semipurified diets containing commercial margarines fortified with either plant stanols (1.1 g/100 g diet) or plant sterols (1.4 g/100 g diet). A reference group of SHRSP rats was fed a soybean oil diet (0.02 g plant sterols/100 g diet and no plant stanols). Compared to soybean oil, both plant stanol and plant sterol margarines significantly (P < 0.05) reduced the life span of SHRSP rats. At the initial stages of feeding, there was no difference in the survival rates between the two margarine groups, but after approximately 50 d of feeding, the plant stanol group had a slightly, but significantly (P < 0.05), lower survival rate. Blood and tissue (plasma, red blood cells, liver, and kidney) concentrations of plant sterols in the plant sterol margarine group were three to four times higher than the corresponding tissue concentrations of plant stanols in the plant stanol group. The deformability of red blood cells and the platelet count of SHRSP rats fed the plant sterol margarine were significantly (P < 0.05) lower than those of the plant stanol margarine and soybean oil groups at the end of the study. These parameters did not differ between the soybean oil and plant stanol margarine groups. These results suggest that, at the levels tested in the present study, plant stanols provoke hemorrhagic stroke in SHRSP rats to a slightly greater extent than plant sterols. The results also suggest that the mechanism by which plant stanols shorten the life span of SHRSP rats might differ from that of plant sterols.

show abstract

Blood coagulation and osmolar tolerance of erythrocytes in stroke-prone spontaneously hypertensive rats given rapeseed oil or soybean oil as the only dietary fat

Cited by 29 publications

References 20 publications

Plant sterol or stanol consumption does not affect erythrocyte osmotic fragility in patients on statin treatment

Plant sterol or stanol consumption does not affect erythrocyte osmotic fragility in patients on statin treatment

Brassica napus

Comparative health effects of margarines fortified with plant sterols and stanols on a rat model for hemorrhagic stroke

Contact Info

Product

Resources

About