2014
DOI: 10.7589/2012-02-047
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BLOOD COLLECTED ON FILTER PAPER FOR WILDLIFE SEROLOGY: DETECTING ANTIBODIES TONEOSPORA CANINUM, WEST NILE VIRUS, AND FIVE BOVINE VIRUSES IN REINDEER

Abstract: We compared Nobuto filter paper (FP) whole-blood samples to serum for detecting antibodies to seven pathogens in reindeer (Rangifer tarandus tarandus). Serum and FP samples were collected from captive reindeer in 2008-2009. Sample pairs (serum and FP eluates) were assayed in duplicate at diagnostic laboratories with the use of competitive enzyme-linked immunosorbent assays (cELISAs) for Neospora caninum and West Nile virus (WNV); indirect ELISA (iELISAs) for bovine herpesvirus type 1 (BHV-1), parainfluenza vir… Show more

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Cited by 19 publications
(27 citation statements)
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“…At each collection, we obtained matched serum and FP sample pairs from each animal. Detailed descriptions of these techniques in free-ranging caribou and captive reindeer have been described previously (Curry et al 2011(Curry et al , 2014.…”
Section: Sampling Methodsmentioning
confidence: 99%
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“…At each collection, we obtained matched serum and FP sample pairs from each animal. Detailed descriptions of these techniques in free-ranging caribou and captive reindeer have been described previously (Curry et al 2011(Curry et al , 2014.…”
Section: Sampling Methodsmentioning
confidence: 99%
“…Those for N. caninum serology were from a Canadian commercial reindeer herd (Curry et al 2014). All other samples were from a previously unvaccinated research reindeer herd in Canada (Curry et al 2014).…”
Section: Design Animals and Antibody Statusmentioning
confidence: 99%
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“…The strips can be stored at room temperature if protected from sunlight, ultraviolet light, extreme temperatures, and moisture (Advantec 2009). When using FP strips for large-scale wildlife disease surveillance, their utility should be evaluated for each host species and disease of interest (Curry et al 2014a). Serosurveillance for canine distemper virus (CDV) and canine parvovirus (CPV-2) has traditionally been conducted using serum.…”
mentioning
confidence: 99%