Blood culture-negative endocarditis

Fournier, Pierre‐Edouard; Gouriet, Frédérique; Casalta, Jean‐Paul; Lépidi, Hubert; Chaudet, Hervé; Thuny, Franck; Collart, Frédéric; Habib, Gilbert; Raoult, Didier

doi:10.1097/md.0000000000008392

Cited by 127 publications

(85 citation statements)

References 29 publications

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“…For example, microbiological criteria for a clinical diagnosis of Bartonella endocarditis in a patient with a blood culture-negative endocarditis (BCNE), include Bartonella spp. PCR positive or an IFA antibody titer of ≥800 against B. henselae or B. quintana antigens [48]. Amplification of B. henselae or B. quintana DNA from excised heart valves also establishes a diagnosis of Bartonella endocarditis in BCNE cases [9,48].…”

Section: Discussionmentioning

confidence: 99%

“…PCR positive or an IFA antibody titer of ≥800 against B. henselae or B. quintana antigens [48]. Amplification of B. henselae or B. quintana DNA from excised heart valves also establishes a diagnosis of Bartonella endocarditis in BCNE cases [9,48]. The prevalence of Bartonella infection using serological techniques is also a cause of concern.…”

Section: Discussionmentioning

confidence: 99%

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Bartonella spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain

et al. 2020

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Bartonella spp. are increasingly implicated in association with a spectrum of zoonotic infectious diseases. One hundred sanitary workers in La Rioja, Spain, completed a questionnaire and provided blood specimens for Bartonella spp. serology and Bartonella Alpha-Proteobacteria growth medium (BAPGM) enrichment blood culture/PCR. Six immunofluorescence assays (IFA) were performed and aseptically obtained blood specimens were inoculated into liquid BAPGM and subcultured onto blood agar plates. Bartonella DNA was amplified using conventional and real-time PCR assays. The Bartonella spp., strain, or genotype was determined by DNA sequencing. Bartonella seroreactivity was documented in 83.1% and bloodstream infection in 21.6% of participants. Bartonella henselae, B. vinsonii subsp. berkhoffii genotypes I and III, and B. quintana were identified. IFA seroreactivity and PCR positivity were not statistically associated with self-reported symptoms. Our results suggest that exposure to and non-clinical infection with Bartonella spp. may occur more often than previously suspected in the La Rioja region.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bartonella spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain

et al. 2020

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“…Infective endocarditis (IE) remains a deadly disease despite significant progress in the diagnostic procedures and therapies. The most common cases of IE involve staphylococci, streptococci and enterococci as the preponderant microorganisms (>88%) recovered from blood cultures (1). The HACEK group ( Haemophilus spp., Aggregatibacter spp., Cardiobacterium spp., Eikenella corrodens , and Klebsiella spp.)…”

Section: Introductionmentioning

confidence: 99%

“…The HACEK group ( Haemophilus spp., Aggregatibacter spp., Cardiobacterium spp., Eikenella corrodens , and Klebsiella spp.) of fastidious oropharyngeal bacteria and other gram-negative organisms were reported to cause infection in 0.9–1.6% and 7.4% of IE patients with positive blood culture, respectively (1, 2).…”

Section: Introductionmentioning

confidence: 99%

“…A recent study reported that in 31% of the 918 cases suspected for IE (1), no causative microorganism could be grown using standard blood culture methods. Nevertheless, causative agents can be identified in ~50% of blood culture-negative patients (3) using other methods, including serology, the analysis of the excised valve by microscopy and PCR, and culture-based analyses of samples from the excised valve and other body sites.…”

Section: Introductionmentioning

confidence: 99%

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Rare Case of Community-Acquired Endocarditis Caused by Neisseria meningitidis Assessed by Clinical Metagenomics

Choutko¹,

Lazarević²,

Gaïa³

et al. 2019

Front. Cardiovasc. Med.

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The most common causes of infective endocarditis (IE) are Staphylococcus, Streptococcus, Enterococcus , and HACEK-related organisms. In 15–30% of the IE cases, standard blood cultures remain sterile. We aimed at identifying the causative agent of a blood-culture-negative IE by whole metagenome shotgun sequencing (WMGS). A 54-year old woman diagnosed with community-onset pneumonia by a general practitioner, was admitted with dyspnea, cough and fever. The patient's blood cultures were repeatedly negative. The transesophageal echocardiography and transthoracic echocardiography showed an echo density on the left coronary leaflet of the aortic valve and signs suggestive of a ruptured abscess of the mitro-aortic junction. The patient underwent a semi-urgent aortic valve replacement by a mechanical prosthetic valve. We extracted DNA from the surgically-removed fresh valve tissue. The extraction procedure included bacterial/fungal DNA enrichment procedure. Nextera XT library prepared from the valve DNA extract was sequenced (2 × 250) on an Illumina MiSeq instrument. Sequence reads were mapped against bacterial genomic sequences, 16S rRNA genes and clade-specific taxonomic markers. Most of the 103,136 sequencing reads classified as bacterial were assigned to Neisseria meningitidis . In line with these data, mapping of reads against clade-specific and 16S rRNA gene markers revealed N . meningitidis as the most represented species. Assembled metagenomic fragments had the best average nucleotide identity (ANI) with N . meningitidis . Comparison of assembled contigs to reference alleles showed that this strain belongs to the ST-41/44 complex. N. meningitidis is commonly associated with meningitis and/or septicemia but should not be neglected as a causative agent of IE, which became exceedingly rare with the introduction of antibiotics. Our data show that WMGS may be used as a diagnostic procedure to strengthen the diagnosis of IE and to obtain draft genomic sequence of the pathogen and typing information.

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Blood culture‐negative infective endocarditis presenting with atypical dermatologic manifestation: A rare case report and review of the literature

Najafizadeh

Dashti

Pahlevani

et al. 2023

Clinical Case Reports

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Infective endocarditis (IE) is characterized by inflammation of the endocardium, including the endocardial wall and heart valves. 1 Blood culture-negative endocarditis (BCNE) is marked by probable or definite endocarditis when three or more blood cultures sampled over 48 h are negative despite adequate incubation (>1 week). It accounts for 2.5%-31% of all cases of IE. 2 Major causes of BCNE include: (1) antibiotic administration prior to sampling (often due to infection with prevalent causative organisms, such as streptococci, staphylococci, or enterococci), (2) presence of fastidious organisms, which are hard to culture. These include Propionibacterium acnes, HACEK bacteria, defective streptococci-Gemella, Abiotrophia sp., Granulicatella, and Candida. (3) the

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Blood culture-negative endocarditis

Abstract: Supplemental Digital Content is available in the text

Cited by 127 publications

References 29 publications

Bartonella spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain

Bartonella spp. Prevalence (Serology, Culture, and PCR) in Sanitary Workers in La Rioja Spain

Rare Case of Community-Acquired Endocarditis Caused by Neisseria meningitidis Assessed by Clinical Metagenomics

Blood culture‐negative infective endocarditis presenting with atypical dermatologic manifestation: A rare case report and review of the literature

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