Blood-Derived Plasma Protein Biomarkers for Alzheimer’s Disease in Han Chinese

Abstract

It is well known that Alzheimer’s disease (AD) is one of the most common progressive neurodegenerative diseases; it begins gradually, and therefore no effective medicine is administered in the beginning. Thus, early diagnosis and prevention of AD are crucial. The present study focused on comparing the plasma protein changes between patients with AD and their healthy counterparts, aiming to explore a specific protein panel as a potential biomarker for AD patients in Han Chinese. Hence, we recruited and collected plasma samples from 98 AD patients and 101 elderly healthy controls from Wuxi and Shanghai Mental Health Centers. Using a Luminex assay, we investigated the expression levels of fifty plasma proteins in these samples. Thirty-two out of 50 proteins were found to be significantly different between AD patients and healthy controls (P < 0.05). Furthermore, an eight-protein panel that included brain-derived neurotrophic factor (BDNF), angiotensinogen (AGT), insulin-like growth factor binding protein 2 (IGFBP-2), osteopontin (OPN), cathepsin D, serum amyloid P component (SAP), complement C4, and prealbumin (transthyretin, TTR) showed the highest determinative score for AD and healthy controls (all P = 0.00). In conclusion, these findings suggest that a combination of eight plasma proteins can serve as a promising diagnostic biomarker for AD with high sensitivity and specificity in Han Chinese populations; the eight plasma proteins were proven important for AD diagnosis by further cross-validation studies within the AD cohort.

Identification of Hub Genes and Key Pathways Associated With Bipolar Disorder Based on Weighted Gene Co-expression Network Analysis

“…In our results, 16 of the 30 hub genes are related to AZ. Among them, AGT, APOE, GJA1, MAOA, RAB31, NOTCH2, PDLIM5, SLC1A3, SMAD2, and YAP1 are biomarkers or related risk factors of AZ (Wu et al, 2004; Ueberham et al, 2009; Kanai et al, 2013; Cheng et al, 2018; Kajiwara et al, 2018; Liang et al, 2018; Quartey et al, 2018; Xu et al, 2018; Zhao et al, 2018). This further proves that there may be a certain relationship between BD and AZ, with overlapping mutant genes.…”

The Plasma Amyloid Beta 42&nbsp;(Aβ42) and Proteomics Profile Related to Canine Cognitive Dysfunction Syndrome (CCDS) in Thailand

“…A previous study in human and transgenic mouse models indicated that the plasma clusterin concentration was signi cantly increased in AD patients and was associated with the level of brillar Aβ in the brain. Moreover, plasma transthyretin levels were also signi cantly increased in comparisons between patients with AD and controls [36,68,69]. Aβ sequester proteins may have a dual function by reducing the formation of toxic species and increasing clearance and degradation through LRP-1-mediated endocytosis [21].…”

“…Proteomics is one of the most signi cant techniques that allows an extended investigation of neurodegenerative diseases. A blood-based proteomics approach was used extensively in humans with AD to study potential biomarkers or mechanisms related to this disease [36][37][38]. Proteomics in AD revealed many interesting proteins or pathways from CSF and blood, such as fatty acid oxidation and the advanced glycation end products/receptors for advanced glycation end products (AGE/RAGE) pathway [39,40].…”

Blood-based protein mediators of senility with replications across biofluids and cohorts

“…Plasma SAP levels are associated with Alzheimer’s disease in Han Chinese ( Cheng et al , 2018 ). However, that finding was age-adjusted and may not be relevant to Aging Proper.…”