Blood Donors with Indeterminate Anti-p24^gag Reactivity in HIV-1
Western Blot: Absence of Infectivity to Transfused Patients and in
Virus Culture

Poel, C.L. van der; Lelie, P.N.; Reesink, H.W.; Exel-Oehlers, P.J.; Tersmette, M.; Akker, Robin van den; Gonzalves, M.; Huisman, J.G.

doi:10.1159/000460955

Cited by 10 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Direct testing for HIV infectivity was carried out by cocultivation of donor-derived lymphocytes with permissive cells. The sum of these data shows that HIV-1 detection was restricted to Western blot analysis only [6,7].…”

Section: Detection Of Hiv-1 Sequences Integrated Inmentioning

confidence: 89%

“…No positive signal was found for any of these donors, either using EtBr staining, blotting and screening or using the LH assay. The complete description of these donors is given by van der Poel et al [6]. Figure 5 shows the negative results of one DNA sample of each of these donors after nested gag PCR followed by LH detection.…”

Section: Detection Of Hiv-1 Sequences In Blood Samples Of Indeterminamentioning

confidence: 99%

“…These data are (1) the donors belong to a low-risk group for HIV-1 infection; (2) none of the donors has shown full serocon version after more than 1 year of follow-up, and ( 3J blood from 6 of these donors had been transfused before in troduction of HIV-1 testing in the blood bank, and the 10 recipients of this blood have remained seronegative for anti-HIV-1 up to the present time [6]. The persistent seropositivity for the HIV-1 gag product.…”

Section: Detection Of Hiv-1 Sequences Integrated Inmentioning

confidence: 99%

“…Another problem for blood banks is presented by the existence of blood donors whose serum shows persistent anti-p24gag reactivity in Western blot, suggesting the presence of antibodies to HIV-1 core proteins [6]. Mononuclear cells isolated from blood samples of these donors have been found to be nega tive for both virus culture and for the presence of viral antibodies, using a very sensitive radioimmunoprécipita tion assay [6,7], To study the prevalence of unidentified HIV-l-infected persons, but also for confirmation purposes in case of in determinate serological results, alternative techniques with very high sensitivity are required. The polymerase chain reaction (PCR) fits this demand.…”

Section: Introductionmentioning

confidence: 99%

“…The isolated DNA was dissolved in 250 pi 10 mM Trìs-HCl, 1 mM ED TA. Of this, 5 pi was used per PCR assay, DNA was isolated according to the same protocol [12] from fresh CPD-A1 blood and stored Ficoll gradient-isolated peripheral blood lymphocytes of 11 blood donors with persistent indeterminate antip248ae reactivity in Western blot [6,7]. DNA was also isolated from blood samples of 18 …”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Enhanced Detection of HIV-1 Sequences Using Polymerase Chain Reaction and a Liquid Hybridization Technique

Bruisten¹,

Koppelmann²,

Poel³

et al. 1991

Vox Sanguinis

Self Cite

View full text Add to dashboard Cite

In this report we describe a sensitive HIV-1 detection method which is applicable for confirmation testing of donors whose blood sample gives indeterminate viral-serology results. The method involves performing a polymerase chain reaction (PCR) and detecting the generated fragments using liquid hybridization and gel retardation. We found that it is as specific as blotting on a filter and hybridization with an internal probe but at least tenfold more sensitive. After applying it on DNA samples of a panel of 11 persistent indeterminate anti-p24^gag-reactive donors, none was found to be PCR positive. Considering other negative virological and biochemical test results and case-historical data, these donors are not likely to be HIV-1 infected.

show abstract

Section: Detection Of Hiv-1 Sequences Integrated Inmentioning

confidence: 89%

Section: Detection Of Hiv-1 Sequences In Blood Samples Of Indeterminamentioning

confidence: 99%

Section: Detection Of Hiv-1 Sequences Integrated Inmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Enhanced Detection of HIV-1 Sequences Using Polymerase Chain Reaction and a Liquid Hybridization Technique

Bruisten¹,

Koppelmann²,

Poel³

et al. 1991

Vox Sanguinis

Self Cite

View full text Add to dashboard Cite

show abstract

Indeterminate western blot patterns in a cohort of individuals at high risk for human immunodeficiency virus (HIV-1) exposure

et al. 1992

View full text Add to dashboard Cite

Our objective was to map serial patterns of Western blot reactivity over time of a cohort of initially ELISA-negative, Western blot-indeterminate individuals from a high-risk group and to determine if these individuals were at increased risk of harboring occult HIV-1 infection. A 2-year prospective study used serial ELISA, two types of Western blot, immunologic profiles, HIV-1 culture, and analysis by polymerase chain reaction. Subjects were 20 ELISA-negative, Western blot indeterminate homosexual volunteers and 20 matched seronegative controls. Results showed that 19 of 20 study subjects completed a mean of 17.0 months of clinical and laboratory follow-up. Reactivities with p24 and/or with p55 were the two most commonly observed Western blot patterns, occurring in 70% of individuals. Specific Western blot reactivity was dependent upon the particular immunoblot preparation being used and varied considerably on a longitudinal basis. No individual pattern appeared predictive of an increased likelihood of subsequent seroconversion to HIV-1 relative to controls. By all other criteria including polymerase chain reaction analysis, samples from 17 of 19 individuals remained negative for HIV-1 at each time point. Two individuals evolved from an indeterminate to a positive Western blot and, simultaneously, from a negative to a positive polymerase chain reaction analysis, during follow-up. Our conclusions were as follows. ELISA-negative, Western blot-indeterminate individuals from a high-risk group show marked variability in immunoblot findings over time, and these patterns do not appear predictive of an increased likelihood of infection.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Evaluation of a line immunoassay for the differential detection of antibodies to human immunodeficiency virus

Vranckx

1990

Eur. J. Clin. Microbiol. Infect. Dis.

View full text Add to dashboard Cite

A newly introduced single strip line immunoassay was evaluated as a possible alternative to Western blot for the differential detection of antibodies to multiple HIV-1 and HIV-2 antigens in human serum. In a panel of 153 sera found to be positive twice in currently used screening tests, the line immunoassay and the Western blot detected 44 true positive results. Using Western blot as reference method, the line immunoassay gave 100% specificity and sensitivity. These results suggest that the line immunoassay may be a viable alternative to Western blot.

show abstract

Blood Donors with Indeterminate Anti-p24^gag Reactivity in HIV-1 Western Blot: Absence of Infectivity to Transfused Patients and in Virus Culture

Cited by 10 publications

References 12 publications

Enhanced Detection of HIV-1 Sequences Using Polymerase Chain Reaction and a Liquid Hybridization Technique

Enhanced Detection of HIV-1 Sequences Using Polymerase Chain Reaction and a Liquid Hybridization Technique

Indeterminate western blot patterns in a cohort of individuals at high risk for human immunodeficiency virus (HIV-1) exposure

Evaluation of a line immunoassay for the differential detection of antibodies to human immunodeficiency virus

Contact Info

Product

Resources

About