Blood exosomal micro ribonucleic acid profiling reveals the complexity of hepatocellular carcinoma and identifies potential biomarkers for differential diagnosis

Abstract

BACKGROUND Hepatocellular carcinoma (HCC) is one of the leading causes of cancer-related deaths worldwide, but there is a shortage of effective biomarkers for its diagnosis. AIM To explore blood exosomal micro ribonucleic acids (miRNAs) as potential biomarkers for HCC diagnosis. METHODS T RESULTS The principal component analysis suggested that daily alcohol consumption could alter the blood exosomal miRNA profiles of hepatitis B virus positive non-HCC patients through miR-3168 and miR-223-3p. The miRNA profiles also revealed the tumor stages of HCC patients. High expression of miR-455-5p and miR-30c-5p, which significantly correlated with better overall survival in tumor tissues, could also be detected in blood exosomes. Two pairs of miRNAs (miR-584-5p/miR-106-3p and miR-628-3p/miR-941) showed a 94.1% sensitivity and 68.4% specificity to differentiate HCC patients from non-HCC patients. The specificity of the combination was substantially influenced by alcohol consumption habits. CONCLUSION This study suggested that blood exosomal miRNAs can be used as new non-invasive diagnostic tools for HCC. However, their accuracy could be affected by tumor stage and alcohol consumption habits.

Knockdown of long noncoding RNA HUMT inhibits the proliferation and metastasis by regulating miR-455-5p/LRP4 axis in hepatocellular carcinoma

“…In addition, we searched and confirmed the target relationship between miR-455-5p and LRP4 by bioinformatics analysis and luciferase reporter assay. Functional analysis showed that cell proliferation, invasion, and migration were promoted by lowexpression of miR-455-5p in HCC cells, which is also consistent with former researches [ 29 , 44 ]. Under the regulation of miR-140-5p, LRP4 accelerated cell migration and invasion in gastric cancer [ 33 ].…”

The social and occupational consequences of the COVID-19 pandemic among patients with multiple sclerosis in three distinct populations: A web-based cross-sectional survey

“…However, the interesting finding was that the relatives and friends of Spanish MS patients have been more susceptible to COVID-19; the proportion is 32.7% in Spain, 14.2% in Cuba, and 0.52% in China, The possible reason could be widespread of COVID-19 in Spain. 27 Since Wuhan released from quarantine on April 8, the strict control of anti-epidemic measures by the Chinese government, there have been almost no other cases of MS with COVID-19 infection apart from sporadic cases in the past six months.…”

Salivary miR-30c-5p as Potential Biomarker for Detection of Oral Squamous Cell Carcinoma

“…miR-30c-5p studied here has been shown to be involved in other cancers such as colorectal cancer [ 32 , 33 ], non-small cell lung cancer [ 34 , 35 ], gastric cancer [ 36 ], pancreatic ductal adenocarcinoma [ 37 ], renal cell carcinoma [ 38 , 39 ], hepatocellular carcinoma [ 40 , 41 , 42 , 43 ], breast cancer [ 44 , 45 , 46 , 47 , 48 ], laryngeal cancer [ 49 ], and other malignancies. For example, in gastric cancer, miR-30c-5p suppresses migration, invasion, and epithelial to mesenchymal transition via targeting MTA1 [ 36 ].…”