Blood extraction method by endocranial venous sinuses puncture in hunted wild ruminants

Jiménez‐Ruiz, Saúl; Arenas-Montes, Antonio; Cano‐Terriza, David; Paniagua, Jorge; Pujols, Joan; Miró, F.; Fernández-Aguilar, Xavier; González, Miguel Ángel; Franco, Juan José; García‐Bocanegra, Ignacio

doi:10.1007/s10344-016-1056-3

Cited by 24 publications

(27 citation statements)

References 5 publications

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“…Blood samples were collected from hunted red deer (n = 1063), wild boar (n = 666), fallow deer (Dama dama, n = 294), European mouflon (Ovis aries, n = 216), Spanish ibex (Capra pyrenaica hispanica, n = 90) and roe deer (Capreolus capreolus, n = 22) ( Table 1). Samples were obtained from the thoracic cavity or by endocranial venous sinuses puncture (Arenas-Montes et al 2013;Jiménez-Ruiz et al 2016).…”

Section: Methodsmentioning

confidence: 99%

Toxoplasma gondii in sympatric domestic and wild ungulates in the Mediterranean ecosystem

et al. 2018

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Toxoplasma gondii is a zoonotic protozoan of worldwide distribution. The present study provides information on risk factors affecting T. gondii infection in domestic and free-ranging wild ungulates sharing habitats in Mediterranean ecosystems in Spain. Serum samples from 482 extensively reared domestic ruminants and 2351 wild ungulates were tested for T. gondii antibodies using the modified agglutination test (MAT, cut-off 1:25). Toxoplasma gondii seroprevalence was 41.2% of 194 sheep, 18.6% of 199 cattle and 5.6% of 89 goats. The main risk factors associated with infection in livestock were the presence of cats, feeding on the ground and at stubble fields. In wild ungulates, T. gondii antibodies were detected in 10.5% of 1063 red deer, 15.6% of 294 fallow deer, 5.6% of 216 European mouflon, 5.6% of 90 Spanish ibex, 13.6% of 22 roe deer and 18.6% of 666 wild boars. The risk factors affecting T. gondii infection in wildlife were species, age and hunting season. Significantly higher seroprevalence was found in domestic ruminants, particularly in sheep, compared to the wild species tested. The present study indicates widespread exposure to T. gondii among domestic and wild ungulates in Southern Spain, with significant differences among species sharing the same ecosystem. The high seroprevalence observed in domestic ruminants, particularly in sheep, reinforces the need for farm management practices to control the risk factors associated with T. gondii infection in extensively reared livestock. Consumption of raw and undercooked food products from domestic and wildlife species may have important implications for public health.

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Section: Methodsmentioning

confidence: 99%

Toxoplasma gondii in sympatric domestic and wild ungulates in the Mediterranean ecosystem

et al. 2018

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“…Blood samples were taken by endocranial venous sinus puncture (Arenas‐Montes et al., ; Jiménez‐Ruiz et al., ). Samples were collected into sterile tubes without anticoagulant and centrifuged at 400 g for 10 min.…”

Section: Methodsmentioning

confidence: 99%

“…Blood samples were taken by endocranial venous sinus puncture(Arenas-Montes et al, 2013;Jim enez-Ruiz et al, 2016). Samples…”

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confidence: 99%

Management of hunting waste as control measure for tuberculosis in wild ungulates in south-central Spain

Cano‐Terriza

Risalde

Jiménez‐Ruiz

et al. 2018

Transbound Emerg Dis

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In recent decades, habitat change and the intensive management of wild ungulates for hunting have led to an increase in their populations in south-central Spain. This implies a higher generation of hunting waste, which can favour the transmission of infectious diseases, including tuberculosis (TB). The aim of this study was to assess the usefulness of the proper disposal of hunting waste as TB control measure in wild boar (Sus scrofa) and red deer (Cervus elaphus) during the 2008/2009 to 2016/2017 hunting seasons. Blood samples from 664 wild boar and 934 red deer were obtained in 14 game estates in two provinces in Andalusia (Area 1), where the disposal of hunting waste was implemented since the 2012/2013 hunting season. Besides, six game estates in the province of Ciudad Real, in Castilla-La Mancha (Area 2), an adjacent region where this management measure was not implemented during the studied period, were used as controls, sampling 277 wild boar and 427 red deer sera. The Mycobacterium tuberculosis complex (MTC), seroprevalence detected in wild boar from Area 1, was significantly higher before the disposal of big game hunting by-products (82.8%; 2008/2009-2012/2013) compared to the second period (61.8%; 2013/2014-2016/2017) (p < .001), after this control measure became established. By contrast, no significant differences between periods were found in wild boar (41.3% versus 44.8%; p = .33) and red deer (14.9% versus 11.6%; p = .19) from Area 2 as well as in red deer (10.8% versus 10.5%; p = .48) from Area 1. The proper disposal of hunting waste contributed to achieve a 25% reduction in MTC seroprevalence in wild boar. These results are of particular relevance regarding wild boar in the current context of re-emerging and emerging diseases such as TB and African Swine Fever in Europe. Further studies are needed to assess the effect of this measure on the health status of livestock and other wildlife species.

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“…All samples were classified according to sex. Blood samples were taken from the thoracic cavity or by puncture of the dural venous sinuses, as previously described [ 19 , 20 ]. Samples were placed into sterile tubes and centrifuged at 400 g for 15 minutes.…”

Section: Methodsmentioning

confidence: 99%

Monitoring of Schmallenberg virus in Spanish wild artiodactyls, 2006–2015

et al. 2017

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Schmallenberg disease is an emerging disease that affects domestic and wild ruminants in Europe. An epidemiological survey was carried out to assess exposure to Schmallenberg virus (SBV) in wild artiodactyls in Spain between 2006 and 2015. A total of 1751 sera from wild artiodactyls, including 1066 red deer, 304 fallow deer, 192 mouflon, 109 wild boar, 49 roe deer and 31 Spanish ibex were tested for antibodies against SBV by ELISA and confirmed by virus neutralization test. SBV was not detected between the 2006/2007 and the 2010/2011 hunting seasons. Overall seroprevalence (including samples collected between the 2011/2012 and 2014/2015 hunting seasons) was 14.6% (160/1099; 95%CI: 12.7–16.6). Mean SBV seroprevalence was 13.3±2.6% in red deer, 23.9±4.2% in fallow deer, 16.4±6.1% in mouflon and 2.8±3.1% in wild boar. No antibodies against SBV were found in roe deer or Spanish ibex. The presence of SBV RNA was confirmed in three of 255 (1.2%) spleen samples from wild ruminants analysed by rRT-PCR. In a multivariate mixed-effects logistic regression model, the main risk factors associated with SBV seroprevalence were: species (fallow deer, red deer and mouflon), age (adults) and interactions between hunting areas of more than 1000 hectares and hunting season (2012/2013, 2013/2014 and 2014/2015). The hypothesis of endemic circulation of SBV in the last few years is supported by the detection of SBV RNA in animals sampled in 2011 and 2015, as well as antibodies detected at low level in juveniles in 2012, 2013 and 2014. The results indicate that SBV circulated in wild ruminant populations in Spain during the same period when the virus was first reported in northern Europe, and at least five months before the first case was officially reported in livestock in Spain.

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Blood extraction method by endocranial venous sinuses puncture in hunted wild ruminants

Cited by 24 publications

References 5 publications

Toxoplasma gondii in sympatric domestic and wild ungulates in the Mediterranean ecosystem

Toxoplasma gondii in sympatric domestic and wild ungulates in the Mediterranean ecosystem

Management of hunting waste as control measure for tuberculosis in wild ungulates in south-central Spain

Monitoring of Schmallenberg virus in Spanish wild artiodactyls, 2006–2015

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