Blood Flow and Ovarian Response After the Use of Short-Term Protocol to Synchronize Ovulation in Goats

Abstract

This study was designed to investigate the relationship between progesterone (P4), estradiol (E2), nitric oxide (NO), and blood flow (BF) of ovarian follicle after estrous synchronization in Sa'idi goats. Thirty goats were injected with PGF2α , then immediately after they received an intravaginal Progestagen impregnated sponge for 5 days. At the time of sponge removal, the goats were divided into two groups; group 1 was injected with PMSG and group 2 was injected with GnRH. The day of Progestagen withdrawal was considered as Day 0.Arbitrary-scale, blood flow area (BA) and blood flow area percent (BA%) were used to assess the follicle blood flow (FBF) in color Doppler images. The results showed that follicular wave emergence occurred in PMSG group significantly earlier (P<0.05) than in GnRH group. There was a significant difference between groups (P<0.001) and group by day interaction (P<0.001) in BA and BA%. The P4 and E2 levels showed an effect of group (P<0.001) and effect of group by day interaction (P<0.001).The NO revealed only a significant difference between group by day interaction (P<0.001). The increase in E2 was associated with a marked increase in NO and follicular growth. In both groups, P4 was negatively correlated with NO; however, the E2 and BF were positively correlated with NO. All correlations were significant (P < 0.0001).The data of the present study, concluded a positive correlation between E2, NO and BF in goats.