Blood gas analysis of bovine fetal capillary blood during stage II labor

Evaluation of the i‐STAT portable point‐of‐care analyzer for determination of blood gases and acid–base status in newborn calves

“…†Clinical limits determined previously at the clinic of reproductive medicine using the same reference methods . No clinical limits for TCO 2 was available for newborn calves.…”

“…† Clinical limits determined previously at the clinic of reproductive medicine using the same reference methods. 19 No clinical limits for TCO 2 was available for newborn calves. PvCO 2 , partial pressure of carbon dioxide in venous blood; PvO 2 , partial pressure of oxygen in venous blood; BE, base excess; HCO 3 , bicarbonate concentration; SvO 2 , venous saturation of oxygen; TCO 2 , total carbon dioxide.…”

“…The difference between the i‐Stat and Rapidlab 248 is then expected to be within the interval of 0 ± 1.96 *CV* mean in 95% of the measurements. To minimize the effect of systematic differences and to transfer the reference intervals (mean ± 2SD) for newborn calves obtained from the Rapidlab 248 in a previous study to the i‐STAT, the differences of the mean were used to calculate the values of the i‐STAT . For variables with a distinct systematic proportional error (PO 2 and lactate), slopes and intercepts of the regression equation were used to adjust the results of the reference methods.…”

“…Additionally, clinical acceptable limits were defined using the SD of the variables measured with the Rapidlab 248 in healthy newborn calves in the above mentioned study (pH ± 0.13, PvCO 2 ± 12.5 mm Hg, PvO 2 ± 5.8 mm Hg, HCO 3 ± 4.0 mmol/L, BE(ecf) ± 6.0 mmol/L, sO 2 ± 9.2%, l ‐lactate ± 2.46 mmol/L) …”

“…Additionally, clinical acceptable limits were defined using the SD of the variables measured with the Rapidlab 248 in healthy newborn calves in the above mentioned study (pH ± 0.13, PvCO 2 ± 12.5 mm Hg, PvO 2 ± 5.8 mm Hg, HCO 3 ± 4.0 mmol/L, BE(ecf) ± 6.0 mmol/L, sO 2 ± 9.2%, L-lactate ± 2.46 mmol/L). 19 To determine the clinical relevance of i-STAT measurements, reference ranges (mean ± 2SD) for newborn calves obtained from the Rapidlab 248 were used for comparison. 19 Values obtained by the i-STAT and the reference methods that were outside the reference range were identified and compared to determine whether data of the reference methods outside the reference range were accompanied by corresponding i-STAT measurements outside the reference range.…”