Blood Glucose, Insulin and Inorganic Phosphorus in Healthy and Ketotic Dairy Cows after Intravenous Infusion of Glucose Solution

Đoković, Radojica; Šamanc, Horea; Ilić, Zoran; Kurčubić, Vladimir

doi:10.2754/avb200978030449

Cited by 11 publications

(13 citation statements)

References 12 publications

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“…of glucose was 143.7 pmol/L of insulin per 1 mmol/L of glucose in heathy cow vs. 107.14 pmol/L of insulin per 1 mmol/L of glucose in ketotic cow). Other authors have reported similar results (SAKAI et al, 1993;SAKAI et al1996;HOLTENIUS et al, 2003;DJOKOVIĆ et al, 2009). All of this shows that Pi response depends on insulin response to glucose, that is the presence of insulin resistance.…”

Section: R Cincović Et Al: Inorganic Phosphorus Decrease Is Assosupporting

confidence: 66%

Inorganic phosphorus decrease after intravenous glucose tolerance test is associated with insulin resistance in dairy cows

Cincović¹,

Đoković²,

Belić³

et al. 2017

Vet. arhiv

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: Inorganic phosphorus decrease after intravenous glucose tolerance test is associated with insulin resistance in dairy cows. Vet. arhiv 87, 409-418, 2017. ABSTRACTInorganic phosphorus (Pi) concentration in blood decreases during an intravenous glucose tolerance test (IVGTT) due to the increase in the level of insulin and glucose. The objective of the present study was to determine the relationship between the intensity of Pi decrease with a dynamic change of insulin and glucose during IVGTT (AUC -total area under curve, AUC increment -area under curve from start of IVGTT to time of maximal response and glucose CR-clearance rate), as well as RQUICKI (Revised Quantitative Insulin Sensitivity Check Index) and RQUICKI-BHB (RQUICKI with beta hydroxybutyrate in formula) indexes of insulin resistance. The experiment included healthy and ketotic cows. Metabolic changes in ketotic cattle are similar to healthy cows in early lactation; ketosis represents impaired metabolic adaptation with higher insulin resistance. In both groups we found increases in insulin and glucose concentrations and Pi AUC during IVGTT, but these changes were less pronounced in the ketotic group. Ketotic cows showed a lower RQUICKI and RQUICKI-BHB index which indicates a higher level of insulin resistance. Pi AUC was in positive correlation with glucose response (glucose AUC), and this correlation is controlled with insulin response to glucose (insulin AUC). Pi AUC also showed a positive correlation with maximal glucose and insulin concentration during IVGTT, AUC and AUC increment for glucose and insulin, and glucose CR. These correlations were controlled by RQUICKI and RQUICKI-BHB indexes. The Pi decrease after the intravenous glucose tolerance test in dairy cows is associated with all aspects of insulin resistance, that include the insulin response to glucose (insulin AUC) and tissue response to insulin (RQUICKI and RQUICKI-BHB).

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Section: R Cincović Et Al: Inorganic Phosphorus Decrease Is Assosupporting

confidence: 66%

Inorganic phosphorus decrease after intravenous glucose tolerance test is associated with insulin resistance in dairy cows

Cincović¹,

Đoković²,

Belić³

et al. 2017

Vet. arhiv

Self Cite

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“…Serum insulin levels showed a significant decrease in ketotic cows compared with control cows. Decreased serum insulin in ketotic cows is agreeable to that recorded by Sadeghi et al 2011and Djoković et al 2009.Moreover, the decreased serum insulin in ketotic cows is agreeable to that recorded by Ahmad et al, (2005); and Teli and Ali, (2007) in ketotic buffaloes. Decreased serum insulin levels could be attributed to the decreased ability of β-cells of the endocrine pancreas to synthesize and release insulin (Dokovic et al, 1998 andDjoković et al, 2009).…”

Section: Discussionsupporting

confidence: 89%

“…Decreased serum insulin in ketotic cows is agreeable to that recorded by Sadeghi et al 2011and Djoković et al 2009.Moreover, the decreased serum insulin in ketotic cows is agreeable to that recorded by Ahmad et al, (2005); and Teli and Ali, (2007) in ketotic buffaloes. Decreased serum insulin levels could be attributed to the decreased ability of β-cells of the endocrine pancreas to synthesize and release insulin (Dokovic et al, 1998 andDjoković et al, 2009). Low plasma insulin concentration reduces glucose uptake by nonmammary extrahepatic tissue and makes glucose available for uptake by the mammary gland which is not responsive to insulin (Bauman.,2000).…”

Section: Discussionsupporting

confidence: 89%

Clinico-biochemical, oxidative markers and trace elements changes in cows with ketosis

Ghanem¹,

Fares

Abdel-Raof

et al. 2017

Benha Veterinary Medical Journal

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Ketosis was diagnosed in 10 lactating cows in private farm in Qaluabia government based on the blood level of Non-esterified fatty acid (NEFA) and Beta hydroxy butyric acid (BHBA). Another 10 healthy cows with normal NEFA and BHBA were used as control. Clinically, the affected cows had partial or complete anorexia with reduction of rumen movement. Biochemically, there was a significant (P< 0.05) increase in AST, ALT, creatinine in addition to NEFA and BHBA in ketotic group compared to control. On the other hand, there was significant decrease (P < 0.05) in triacyl glyceride, albumin, copper and zinc. The oxidative stress markers showed significant increase (P <0.05) in MDA concentration and significant decrease in SOD activity. It was concluded that ketosis is related to clinical, biochemical, oxidative stress and reduced serum Cu and Zn levels. It is recommended to add trace elements, specially serum Cu and Zn in the periparturient period.

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“…Hayirli (2006) and Koster and Opsomer (2013) have shown that low insulin concentrations exacerbate lipid mobilization and increase the plasma concentrations of NEFA, which in turn inhibit insulin-dependent glucose uptake in peripheral tissues by decreasing the expression of GLUT4, consequently reducing the sensitivity of these tissues to insulin. Another contributing factor is the relationship between the hypoinsulinemia and the ability of β-pancreatic cells to produce and release this hormone during hypoglycemia at disease onset (DJOKOVIĆ et al, 2009;2014). Hypoinsulinemia is directly associated with the increase in serum concentrations of NEFA, suggesting that the reduced anabolic effect of insulin on lipid metabolism results in the exacerbated mobilization of NEFA from body stores.…”

Section: Clinicalmentioning

confidence: 99%

Cardiac biomarkers and blood metabolites in cows with clinical ketosis

Soares

Ribeiro

Cajueiro

et al. 2019

SCA

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The increase in energy imbalance at the beginning of lactation leads to metabolic disorders, especially clinical ketosis, in highly productive dairy cows. The objective of this study is to evaluate the clinical characteristics, biochemical parameters, and cardiac biomarkers creatine kinase MB isoenzyme (CK-MB) and cardiac troponin I (cTnI) of 15 cows diagnosed with clinical ketosis treated at the Bovine Clinic of Garanhuns at the Federal Rural University of Pernambuco. Nine of the 15 cows were diagnosed with primary ketosis and six had concomitant diseases, including left abomasal displacement, metritis, pneumonia, and renal failure. The observed clinical signs were non-specific and included decreased appetite and milk production and weight loss. Approximately 53% of the animals had central nervous system involvement. For biochemical evaluation, 15 high-production healthy dairy cows at the initial stage of lactation served as the control group. The data were subjected to analysis of variance for simultaneous comparison between two means allowing testing the hypothesis on the means of the different groups and subjected to Pearson correlation at a level of significance of 5%. The levels of ?-hydroxybutyrate (BHB), non-esterified fatty acids (NEFA), glucose, fructosamine, total protein, albumin, creatinine, aspartate aminotransferase, creatine kinase, CK-MB, and cTnI were higher in the group with ketosis. The diseased animals presented changes in glycemia, reduction in tissue sensitivity to insulin, and a lower RQUICKI-BHB index, especially hyperglycemic animals. The increase in the levels of cardiac biomarkers suggests the occurrence of myocardial injury associated with bovine ketosis.

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Blood Glucose, Insulin and Inorganic Phosphorus in Healthy and Ketotic Dairy Cows after Intravenous Infusion of Glucose Solution

Cited by 11 publications

References 12 publications

Inorganic phosphorus decrease after intravenous glucose tolerance test is associated with insulin resistance in dairy cows

Inorganic phosphorus decrease after intravenous glucose tolerance test is associated with insulin resistance in dairy cows

Clinico-biochemical, oxidative markers and trace elements changes in cows with ketosis

Cardiac biomarkers and blood metabolites in cows with clinical ketosis

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