Blood haemogram in <i>Ovis aries</i> and <i>Capra hyrcus</i>: effect of storage time

Fazio, Francesco; Giangrosso, Giuseppe; Marafioti, Simona; Zanghì, Elisa; Arfuso, Francesca; Piccione, Giuseppe

doi:10.1139/cjas-2015-0106

Cited by 9 publications

(6 citation statements)

References 18 publications

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“…Blood smear evaluation is also useful for evaluating other preanalytic artifactual changes, as time and temperature can also affect other cell lines . Numerous reports document time‐ and temperature‐dependent changes in RBCs, WBCs, and PLTs, and automated and manual CBC indices in cattle, lab animals, dogs, sheep, goats, horses, turkeys, and harp seals . However, surprisingly, in Xenopus tropicalis , using the Natt‐Herrick solution, cellular integrity was maintained for 2 years post collection …”

Section: Discussionmentioning

confidence: 99%

“…Evaluation and, therefore, interpretation of CBC findings can be limited by certain preanalytical factors. For example, prolonged storage could cause artifactual increases in hematocrit, MCV, and MPV, and decreases in the MCHC and PLT variables . Additionally, in 1984, Gossett and Carakostas showed that prolonged storage of blood in EDTA could cause the development of cytoplasmic vacuoles in neutrophils.…”

Section: Introductionmentioning

confidence: 99%

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Effect of time and storage on toxic or pseudo‐toxic change in canine neutrophils

Bau‐Gaudreault

Grimes

2019

Veterinary Clinical Pathol

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| INTRODUC TI ONThe CBC is an essential component of patient health evaluations.Automated cell counters have been used since the mid-20th century 1 to rapidly obtain certain hematology data, including several red blood cell (RBC), white blood cell (WBC), and platelet (PLT) indices. However, blood smear evaluation remains a requisite part of the CBC in veterinary species, as automated analyzers do not reliably detect many clinically relevant morphologic abnormalities, such as immature granulocytes, toxic change, infectious agents, and neoplastic cells. Evaluation and, therefore, interpretation of CBC findings can be limited by certain preanalytical factors. For example, prolonged storage could cause artifactual increases in hematocrit, MCV, and MPV, and decreases in the MCHC and PLT variables. 2-6 Additionally, in 1984, Gossett and Carakostas 7 showed that Abstract Background: The presence of toxic change in neutrophils is frequently used as a biomarker of inflammation in dogs. Objective: We aimed to evaluate the effect of time and storage on toxic change in canine neutrophils. Methods: One hundred and fifty microliters of EDTA blood were obtained from eight dogs with no toxic neutrophil changes observed on fresh blood smears (T0). Blood was stored at room temperature (RT), in a box with an icepack (ICE), and at 4°C. For each storage condition, smears were prepared 2 (T2), 4 (T4), 8 (T8), and 24 (T24) hours post blood draw. Smears were randomized, and each smear was evaluated for the presence of toxic neutrophil change. Results: A statistically significant effect of time and storage on the presence of toxic neutrophil change was observed. Compared with T0, the number of neutrophils containing Döhle bodies was significantly higher at T8 and T24 for the RT (P < 0.0001) and ICE (P < 0.0001) samples and at T24 for 4°C samples (P < 0.0001). Additionally, smears were falsely classified as having 1+ toxic change in 0/8 (T2), 1/8 (T4), 3/8 (T8), and 8/8 (T24) for RT samples; 0/8 (T2 and T4), 2/8 (T8), and 5/7 (T24) smears for ICE samples; and 0/8 (T2, T4, and T8) and 2/8 (T24) for 4°C samples. Conclusions: Smears can be falsely classified as having neutrophils with toxic change as early as 4 hours post blood draw in samples stored at RT, 8 hours when stored with icepacks, and 24 hours when stored at 4°C. Canine blood smears should be prepared and evaluated for toxic neutrophil change as early as possible. K E Y W O R D S artifact, delay, Döhle bodies, icepack, preanalytic, refrigerator

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of time and storage on toxic or pseudo‐toxic change in canine neutrophils

Bau‐Gaudreault

Grimes

2019

Veterinary Clinical Pathol

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“…Haematological parameters are generally used to monitor and evaluate the health of small ruminants (Fazio et al, 2016). Due to the economic importance of goats, a lot of information is available on their haematology (Tibbo et al, 2004;Zumbo et al, 2011;.…”

Section: Introductionmentioning

confidence: 99%

Influences of breed, sex and age on seasonal changes in haematological variables of tropical goat kids

et al. 2017

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Abstract. The influences of breed, sex and age on seasonal changes in haematological variables of kids (1-4 months old) belonging to Red Sokoto (n = 60) and Sahel (n = 60) goats were studied at the peaks of the cold-dry, hot-dry and rainy seasons in a West Africa Guinea savanna climate. The results showed that, during the hot-dry season, Sahel goat kids had significantly higher (P < 0.05) packed cell volume (PCV) and red blood cell (RBC) count but lower (P < 0.05) mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and total leucocyte count than Red Sokoto kids. Similarly, younger kids of both breed had significantly higher (P < 0.05) PCV and RBC but lower MCV, MCH and MCHC compared with the older kids during the hot-dry season. Younger kids (1-2 months) of both breeds exhibited significantly (P < 0.05) high PCV and RBC but low MCH and MCHC during the hot-dry season as compared with the cold-dry and rainy seasons, with the magnitude of the change being greater in Sahel kids. More remarkable seasonal fluctuations in haematological parameters were observed in buck kids than doe kids of both breeds. Multivariate analysis revealed a clear distinction between the change in haematological parameters during the cold-dry as compared with the hot-dry and rainy seasons, with MCV having the strongest discriminating power (0.91 * ). In conclusion, breed, age and sex variations in haematological variables of goats were more pronounced in the hot-dry season, during which the seasonal changes were more dramatic in kids of Sahel goats, as well as in younger and male kids of both breeds. These findings may be useful in the management of tropical goat kids in different seasons.

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“…Clotted blood samples were centrifuged2 h after sampling or left at 4°C or 20°C for time intervals of 6h, 12h, 24h, 48h and 72h to simulate potential storage conditions and durations of porcine blood samples before reaching the laboratory. In general, few reports describe effects of temperature and storage duration on biochemical analytes in clotted blood of farm animal species, 11,[15][16][17]25 a couple of these include pigs. [15][16][17] Dissimilarities in e.g.…”

Section: Gamma-glutamyl Transferase (Ggt) and Glutamate Dehydrogenasementioning

confidence: 99%

Untitled

2018

JDVAR

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Knowledge of factors influencing blood sample integrity is important to interpret results correctly. Effects of storage duration at different temperatures on porcine uncentrifuged blood samples are poorly described.The objective of this study were to elucidate effects of storage time and temperature on different porcine biochemical analytes. Multiple clotted, uncentrifuged blood samples from ten gilts were collected and samples were centrifuged 2h after sampling in the laboratory. Remaining samples were stored chilled (at 4±2°C, CT) or at room temperature (20 ± 2°C, RT). Samples were centrifuged 6, 12, 24, 48 or 72hours after arriving at the laboratory. Storage characteristics and stability of albumin (Alb), calcium (Ca), creatinine (Creat), iron (Fe), gamma-glutamyltransferase (GGT), glutamate dehydrogenase (GLDH), glucose (Gluc), magnesium (Mg), inorganic phosphate (P), total protein (TP) and urea were studied. Test repeatability was within acceptable diagnostic limits. A significant time effect was found for Alb (P<0.001), Ca (P<0.0001), Creat (P<0.0001), GLDH (P<0.0001), Gluc (P<0.0001), Mg (P<0.0001), P (P<0.0001) and TP (P<0.0001). Over time, Alb, GLDH, Mg, P and TP increase while Ca, Creat and Gluc decrease. A temperature effect was identified for Ca (P<0.05), GGT (P<0.05), GLDH(P<0.0001), Gluc (P<0.0001), Mg (P<0.0001) and P (P<0.0002). All values, except Gluc, were higher when stored at RT. Gluc values were lower when stored at RT. This study highlights the importance of standardized storage routines of porcine clotted blood samples to ensure the validity of biochemical analyses.

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Blood haemogram in Ovis aries and Capra hyrcus: effect of storage time

Cited by 9 publications

References 18 publications

Effect of time and storage on toxic or pseudo‐toxic change in canine neutrophils

Effect of time and storage on toxic or pseudo‐toxic change in canine neutrophils

Influences of breed, sex and age on seasonal changes in haematological variables of tropical goat kids

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