2020
DOI: 10.7717/peerj.8328
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Blood lactate dynamics in awake and anaesthetized mice after intraperitoneal and subcutaneous injections of lactate—sex matters

Abstract: Lactate treatment has shown a therapeutic potential for several neurological diseases, including Alzheimer’s disease. In order to optimize the administration of lactate for studies in mouse models, we compared blood lactate dynamics after intraperitoneal (IP) and subcutaneous (SC) injections. We used the 5xFAD mouse model for familial Alzheimer’s disease and performed the experiments in both awake and anaesthetized mice. Blood glucose was used as an indication of the hepatic conversion of lactate. In awake mic… Show more

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Cited by 7 publications
(7 citation statements)
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References 29 publications
(34 reference statements)
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“…However, administration of lactate to sham mice had no significant effects on serum lactate levels, vascular permeability, and organ dysfunction. This observation is in agreement with a recent study by Haugen et al ( 49 ) showing that circulating lactate reaches a peak concentration at 13 min and is back to baseline concentrations after 37 min following lactate injection in sham mice.…”
Section: Discussionsupporting
confidence: 94%
“…However, administration of lactate to sham mice had no significant effects on serum lactate levels, vascular permeability, and organ dysfunction. This observation is in agreement with a recent study by Haugen et al ( 49 ) showing that circulating lactate reaches a peak concentration at 13 min and is back to baseline concentrations after 37 min following lactate injection in sham mice.…”
Section: Discussionsupporting
confidence: 94%
“…Table 3 and Table 4 compared the glucose and lactate concentrations obtained from our DWE biosensor with previously reported glucose and lactate concentrations in blood. These values of glucose and lactate concentrations from normal mice and obese mice in our results were comparable with those of previously reported concentrations [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 ]. Increasing ratios were calculated with the following equation: .…”
Section: Resultssupporting
confidence: 93%
“…However, changes in lactate were not significant during obesity monitoring because a relative short term of diet such as 8~12 weeks usually provided a negligible increase (0.5 mM, equivalent to 10% of increasing ratio) in lactate in the case of HFD-induced obesity [ 33 , 36 , 41 ]. Thus, our high increase (2.5 mM, equivalent to 61% of increasing ratio) of lactate in HFD-induced obesity would be a turning point to consider lactate as an important factor for obesity monitoring [ 42 ].…”
Section: Resultsmentioning
confidence: 99%
“…6 h after surgery. The dose of lactate is adjusted based on a previous publication ( 19 ) and was tested on our recent publications ( 12 , 13 ). In addition, this dose of lactate is nonlethal and does not cause acidosis ( 20 ).…”
Section: Methodsmentioning
confidence: 99%