Blood lipids in patients with high and low physical activity depending on polymorphism of beta-2 and 3-adrenergic receptor genes

Abstract

Background. The aim of the study was to investigate the lipid levels in patients with various physical activity and polymorphous variants of ADRB2 (Gln27Glu and Arg16Gly) and ADRB3 (Trp64Arg) genes. Material and methods. One hundred and fifty subjects were enrolled in the study; mean age was 44.7 ± 8.9 y.o. Physical activity was assessed using Omron Walking style III step counter HJ-203-EK pedometer. Lipids were assessed via enzymatic method using Cormay reagent kit (Poland). Genotyping of polymorphous sites of ADRB2 (Gln27Glu, rs1042714 and Arg16Gly, rs1042713) and ADRB3 (Trp64Agr, rs4994) genes was performed using SNP-express-SHOT reagent kits manufactured by Litex Research and Production Company, via real-time polymerase chain reaction. Results. Individuals walking more than 5000 steps daily with Gly16Gly polymorphism had significantly higher blood lipids.