Blood-membrane in teractions during haemodialysis with cellulose and synthetic membranes

Woffindin, C.; Hoenich, Nicholas A.

doi:10.1016/0142-9612(88)90069-5

Cited by 24 publications

(12 citation statements)

References 14 publications

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“…Although cellulose is a well-known material that has been used in dialysis membranes, the literature has suggested that this material is not biocompatible. [31][32][33] Different polyamide membranes have been widely used in implantable devices, but the reported studies concerning the biocompatibility of this material is controversial. Some clinical and in vitro studies (cell cultures) show a good biocompatibility of polyamide, [34][35][36] whereas other studies have claimed this material to be only moderately biocompatible.…”

Section: Discussionmentioning

confidence: 99%

Activation of Polymorphonuclear Leukocytes by Candidate Biomaterials for an Implantable Glucose Sensor

Sokolov

Hellerud

Lambris

et al. 2011

J Diabetes Sci Technol

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Section: Discussionmentioning

confidence: 99%

Activation of Polymorphonuclear Leukocytes by Candidate Biomaterials for an Implantable Glucose Sensor

Sokolov

Hellerud

Lambris

et al. 2011

J Diabetes Sci Technol

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“…It has been reported that the extent of complement activation depends on the type of dialysis membrane [1,[24][25][26] and, in the case of cellulosic membranes, whether the dialyser is new or has been used previously [27,28]. The extent of complement activation seems to correlate well with the severity of symptoms of patients undergoing routine dialysis.…”

Section: Plasminogen 'mentioning

confidence: 99%

“…Dialysis is an established and effective form of treatment for patients suffering from chronic renal failure, although a number of adverse side effects such as chest pain, dyspnea, hypotension, and neutropenia are sometimes encountered [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Identification of proteins adsorbed to hemodialyser membranes from heparinized plasma

Cornelius

Brash

1993

Journal of Biomaterials Science, Polymer Edition

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The protein layers formed during contact of plasma with hemodialysis membranes were studied. Dialysers having membranes of cellulose acetate (CA), saponified cellulose ester (SCE), cuprophane (CUP), polymethylmethacrylate (PMMA), and polyacrylonitrile (PAN) were used. Heparinized human plasma was recirculated through the dialysers for four hours. They were then rinsed and the proteins adsorbed to the membranes were eluted with 2% SDS. The yields of protein from the different membranes increased in the order: PMMA < CA < SCE < CUP < PAN. This is the probable order of increasing hydrophilicity. SDS-PAGE and Western blots were performed on the dialyser eluates. The blots were positive for most of the twenty proteins tested for. There were some interesting differences in adsorption patterns among the different membrane materials, notably for high molecular weight kininogen (HMWK), plasminogen and the C3 component of complement. HMWK was intact in the eluates from CA, CUP and SCE, whereas on PMMA and PAN there was evidence of cleavage, suggesting that activation of the contact phase of coagulation was more extensive on the latter two materials. Intact plasminogen was visible on all the blots. However, low molecular weight fragments were visible in the PAN eluates, suggesting activation of the fibrinolytic pathway. Low molecular weight fibrinogen fragments eluted from PAN membranes support this conclusion. C3 was visible in the blots obtained for all membrane materials, and the data suggest that complement is activated by all the membranes. A C3 fragment at about 30 kD (possibly C3d) was seen in the blots for the cellulosic membranes but not for PMMA or PAN.

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“…This early activation measurements for C3a would also be a good indicator for testing hemodialysis membranes. Reports [2,20] have shown that the first 15-30 min of dialysis might be the critical time where a high amount of C3a generated will induce an extensive leukopenia: this is the case for CDT, while for polyacrylonitrile membrane (AN69) minimal C3a formation had been observed -in fact most of it is adsorbed on its surface -and biological effects such as leukopenia seemed to be absent [21]. Our in vitro results were in accordance with these observations, AN69 was found to be significantly different from CDT and CA.…”

Section: Discussionmentioning

confidence: 99%

“…Complement activation has many clinical consequences; e.g. the leukopenia and hypoxia associated with hemodialysis [2,3]. Some complement products have specific receptors for neutrophils, monocytes, macrophages and mast cells.…”

Section: Introductionmentioning

confidence: 99%

Using Elisa to evaluate complement activation by reference biomaterials

Berger¹,

Broxup²,

Sefton

1994

J Mater Sci: Mater Med

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The interaction between blood and an artificial surface results in the activation of complement and can lead to extensive inflammatory reactions. In vitro work was undertaken to validate a system of assays to evaluate the complement activation of a candidate biomaterial by comparing its capacity of activation with that of reference biomaterials. The biomaterials tested were cellulose acetate, cellulose dialysis tubing, low density polyethylene, polydimethylsiloxane and AN69. Complement activation was assessed using ELISA for iC3b, C3a and Factor Bb. Results showed that (1) iC3b measurements may underestimate complement activation, (2) the presence of Bb in plasma or serum was a reliable indicator of complement activation, (3) measurements of C3a are of interest but are difficult, (4) adsorption or/and binding of complement proteins occured on the surface. Evaluation of complement activation for a biomaterial should include the assessment of the fluid phase (in serum or plasma) as well as the bound phase (on the surface of the biomaterial) of complement.

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Blood-membrane in teractions during haemodialysis with cellulose and synthetic membranes

Cited by 24 publications

References 14 publications

Activation of Polymorphonuclear Leukocytes by Candidate Biomaterials for an Implantable Glucose Sensor

Activation of Polymorphonuclear Leukocytes by Candidate Biomaterials for an Implantable Glucose Sensor

Identification of proteins adsorbed to hemodialyser membranes from heparinized plasma

Using Elisa to evaluate complement activation by reference biomaterials

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