Blood Oxygen Sensor Using a Boron-Doped Diamond Electrode

Abstract

The electrochemical behavior of oxygen (O 2 ) in blood was studied using boron-doped diamond (BDD) electrodes. Cyclic voltammogram of O 2 in a 0.1 M phosphate buffer solution solution containing 1 × 10 −6 M of bovine hemoglobin exhibits a reduction peak at −1.4 V (vs Ag/ AgCl). Moreover, the scan rate dependence was investigated to study the reduction reaction mechanism, which was attributable to the reduction of O 2 to H 2 O 2 via two electrons. A linear calibration curve was observed in the concentration range of 86.88−314.63 mg L −1 (R 2 = 0.99) with a detection limit of 1.0 mg L −1 (S/B = 3). The analytical performance was better than those with glassy carbon or platinum electrodes as the working electrode. In addition, an application to bovine blood was performed. The O 2 concentration in the blood measured on the BDD electrodes was compared to that measured using an OxyLite Pro fiber-optic oxygen sensor device. Both methods gave similar values of the O 2 concentration in the range of ∼40 to 150 mmHg. This result confirms that BDD electrodes could potentially be used to detect the O 2 concentration in blood.

Application of Boron-doped Diamond Electrodes: Focusing on the Electrochemical Reduction of Carbon Dioxide

“…increasing year by year and several review articles and books on diamond-based electrodes have now been published. [1][2][3][4][5][6][7][8][9][10] For the last 20 years, our research group have reported not only fundamental studies on BDD, but also on several novel electrochemical applications such as electrochemical sensors (including biomedical), [11][12][13][14][15][16][17][18] electrochemiluminescence, [19][20][21][22] and electrochemical organic synthesis. [23][24][25] These applications are due to the unique properties of BDD electrodes, and they could not be achieved using conventional electrodes.…”