Blood plasma contact activation on silicon, titanium and aluminium

Arvidsson, Sara; Askendal, Agneta; Tengvall, Pentti

doi:10.1016/j.biomaterials.2006.11.005

Cited by 66 publications

(57 citation statements)

References 20 publications

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“…Slightly higher amounts are found on a-C and CN x as compared to the Ti reference and slightly higher amounts are obtained on the amorphous films following the trend from SE measurements of HSA adsorption. A plasma deposition of ~400 ng/cm 2 on the Ti surface is a reasonable value compared to earlier work [34][35][36].…”

Section: Biointeractions -Human Blood Plasma and Antibody Incubationsupporting

confidence: 66%

Protein adsorption on thin films of carbon and carbon nitride monitored with in situ ellipsometry

et al. 2011

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Amorphous carbon and amorphous, graphitic and fullerene-like carbon nitride thin filmswere deposited by reactive magnetron sputtering and optically characterized withspectroscopic ellipsometry. The films were exposed to human serum albumin and theadsorption was monitored in situ using dynamic ellipsometry. From the ellipsometric data theadsorbed amount of proteins was quantified in terms of surface mass density using de Feijter'smodel. The results indicated larger adsorption of proteins onto the amorphous films comparedto the films with a more ordered microstructure. Complementary studies with labeled HSAusing radioimmunoassay showed up to 6 times higher protein adsorption compared to theellipsometry measurement which partly might be explained by differences in surfaceroughness (from 0.3 to 13 nm) among the films. The elutability of adsorbed labeled HSAusing unlabeled HSA and sodium dodecyl sulphate was low compared to a silicon reference.In addition, the four types of films were incubated in blood plasma followed by antifibrinogen,anti-HMWK or anti-C3c revealing the materials response to complement andcontact activation. Three of the films indicated immunoactivity, whereas the amorphouscarbon showed less immunoactivity compared to a titanium reference. All films showedindications of a stronger ability to initiate the intrinsic pathway of coagulation, compared tothe reference. Finally, the surfaces bone bonding ability was investigated by examination oftheir ability to form calcium phosphate (CaP) crystals in a simulated body fluid, with a-CNxdepositing most CaP after 21 days of incubation.Original Publication:Torun Berlind, Pentti Tengvall, Lars Hultman and Hans Arwin, Protein adsorption on thin films of carbon and carbon nitride monitored with in situ ellipsometry, 2011, ACTA BIOMATERIALIA, (7), 3, 1369-1378.http://dx.doi.org/10.1016/j.actbio.2010.10.024Copyright: Elsevier Science B.V. Amsterdamhttp://www.elsevier.com

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Section: Biointeractions -Human Blood Plasma and Antibody Incubationsupporting

confidence: 66%

Protein adsorption on thin films of carbon and carbon nitride monitored with in situ ellipsometry

et al. 2011

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“…Several in vitro studies have addressed the infl uence of topographical (Park et al, 2001;Di Iorio et al, 2005) and physicochemical surface properties (Nygren et al, 1997;Hong et al, 1999;Eriksson and Nygren, 2001;Hong et al, 2005;Arvidsson et al, 2007) of Ti surfaces Alkali treatment of microrough titanium surfaces on the activation of blood coagulation, the adhesion of blood cells or the adsorption of plasma proteins. These studies indicate that native Ti is intrinsically highly thrombogenic due to strong contact activation (Hong et al, 1998;Hong et al, 1999;Arvidsson et al, 2007). Furthermore, both topographical and physicochemical properties of the surfaces were reported to infl uence the activation of blood coagulation.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, both topographical and physicochemical properties of the surfaces were reported to infl uence the activation of blood coagulation. Surface roughness, e.g., was reported to enhance platelet activation and blood clot retention (Park et al, 2001;Di Iorio et al, 2005;), while the physicochemical properties of Ti surfaces were shown to infl uence contact activation or the adhesion and activation of leukocytes properties (Nygren et al, 1997;Hong et al, 1999;Eriksson and Nygren, 2001;Hong et al, 2005;Arvidsson et al, 2007). However, variation of surface chemistry and topography were often coupled within these studies and experimental conditions and blood products used greatly varied between these studies, which limits their comparability.…”

Section: Discussionmentioning

confidence: 99%

“…Immediately upon implantation medical implants get exposed to the patient's blood and surface interactions between blood components such as blood cells and fi brin(ogen) will infl uence the extent of blood coagulation, fi brin fi ber formation and acute infl ammation (Anderson, 2001;Arvidsson et al, 2007). Activation of these processes might directly trigger the extrinsic coagulation cascade induced by the trauma of the implantation.…”

Section: Introductionmentioning

confidence: 99%

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Alkali treatment of microrough titanium surfaces affects macrophage/monocyte adhesion, platelet activation and architecture of blood clot formation

Milleret

Tugulu²,

Schlottig³

et al. 2011

eCM

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Titanium implants are most commonly used for bone augmentation and replacement due to their favorable osseointegration properties. Here, hyperhydrophilic sand-blasted and acid-etched (SBA) titanium surfaces were produced by alkali treatment and their responses to partially heparinized whole human blood were analyzed. Blood clot formation, platelet activation and activation of the complement system was analyzed revealing that exposure time between blood and the material surface is crucial as increasing exposure time results in higher amount of activated platelets, more blood clots formed and stronger complement activation. In contrast, the number of macrophages/monocytes found on alkali-treated surfaces was signifi cantly reduced as compared to untreated SBA Ti surfaces. Interestingly, when comparing untreated to modifi ed SBA Ti surfaces very different blood clots formed on their surfaces. On untreated Ti surfaces blood clots remain thin (below 15 mm), patchy and non-structured lacking large fi brin fi ber networks whereas blood clots on differentiated surfaces assemble in an organized and layered architecture of more than 30 mm thickness. Close to the material surface most nucleated cells adhere, above large amounts of non-nucleated platelets remain entrapped within a dense fi brin fi ber network providing a continuous cover of the entire surface. These fi ndings might indicate that, combined with fi ndings of previous in vivo studies demonstrating that alkali-treated SBA Ti surfaces perform better in terms of osseointegration, a continuous and structured layer of blood components on the blood-facing surface supports later tissue integration of an endosseous implant.

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“…It induces the fibrin clot formation, which plays a crucial role in implant osseointegration [1]. Since it has been reported that implants surface properties can modulate the extension of the above-mentioned fibrin network [2,3], they have to be considered among the factors that influence the early phase of osseointegration [4].…”

Section: Introductionmentioning

confidence: 99%

The Surface Anodization of Titanium Dental Implants Improves Blood Clot Formation Followed by Osseointegration

et al. 2018

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Abstract:The anodization of titanium dental implant influences the biologic processes of osseointegration. 34 grit-blasted and acid-etched titanium specimens were used to evaluate microand nano-roughness (R a ), contact angle (θ) and blood clot extension (bce). 17 samples were anodized (test) while the remaining were used as control. The bce, was measured using 10 µL of human blood left in contact with titanium for 5 min at room temperature. The micro-and nano-scale R a were measured under CLSM and AFM, respectively, while the θ was analyzed using the sessile drop technique. The bone-implant contact (BIC) rate was measured on two narrow implants retrieved for fracture. bce was 42.5 (±22) for test and 26.6% (±13)% for control group (p = 0.049). The micro-R a was 6.0 (±1.5) for the test and 5.8 (±1.8) µm for control group (p > 0.05). The θ was 98.5 • (±18.7 • ) for test and 103 • (±15.2 • ) for control group (p > 0.05). The nano-R a was 286 (±40) for the test and 226 (±40) nm for control group (p < 0.05). The BIC rate was 52.5 (±2.1) for test and 34.5% (±2.1%) for control implant (p = 0.014). (Conclusions) The titanium anodized surface significantly increases blood clot retention, significantly increases nano-roughness, and favors osseointegration. When placing dental implants in poor bone quality sites or with immediate loading protocol anodized Ti6Al4V dental implants should be preferred.

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Blood plasma contact activation on silicon, titanium and aluminium

Cited by 66 publications

References 20 publications

Protein adsorption on thin films of carbon and carbon nitride monitored with in situ ellipsometry

Protein adsorption on thin films of carbon and carbon nitride monitored with in situ ellipsometry

Alkali treatment of microrough titanium surfaces affects macrophage/monocyte adhesion, platelet activation and architecture of blood clot formation

The Surface Anodization of Titanium Dental Implants Improves Blood Clot Formation Followed by Osseointegration

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