Blood pressure effects of renin inhibition by human renin antiserum in normotensive marmosets

Jb, Michel; Wood, J. M.; Hofbauer, K. G.; Corvol, Pierre; Ménard, Joël

doi:10.1152/ajprenal.1984.246.3.f309

Cited by 22 publications

(21 citation statements)

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“…They are not directed against the active site itself. (7) and marmoset monkeys (8). Preliminary results show that the 4G1 antibody was also able to decrease the blood pressure of conscious salt-depleted marmosets (unpublished data).…”

Section: Discussionmentioning

confidence: 84%

“…Its purification from a juxtaglomerular cell tumor (1) and from cadaver kidney (2,3) facilitated the characterization of the enzyme and the generation of polyclonal antibodies in the rabbit. Polyclonal antibodies against human renin were used to set up a direct radioimmunoassay (RIA)' (4), to localize renin by immunohistochemistry (5,6), and to block in vivo the renin enzymatic activity in an experimental model of hypertension in the monkey (7,8). There are, however, several limitations in the use of antirenin polyclonal antibodies: The scarcity of pure human renin means that the supply of antisera is limited and that the purification of antibodies on a renin affinity column is not feasible.…”

Section: Introductionmentioning

confidence: 99%

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New monoclonal antibodies directed against human renin. Powerful tools for the investigation of the renin system.

Galen¹,

Devaux²,

Atlas³

et al. 1984

J. Clin. Invest.

134

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A bstract. Monoclonal antibodies directed against human renin were obtained by the fusing of myeloma cells with spleen cells from Balb/c or highresponder Biozzi mice injected with pure tumoral or highly purified renal renin. These procedures resulted in the production of seven stable monoclonal antibodies to human renin. Antibodies in the hybridoma culture medium were screened by binding to pure iodinated renin or insolubilized renin in a solid phase assay. The concentration of purified antibodies that provided a 50% binding to iodinated renin varied from 1 X 10-10 to 1 X 10-7 M. Two monoclonal antibodies were found to be potent inhibitors of renin enzymatic activity in vitro, behaving as noncompetitive inhibitors (Ki, 1 to 4 X 10`s M). They were specific for primate renin. Three monoclonal antibodies provided suitable immunoadsorbants for renin purification. One of these immunoadsorbants was used for large-scale purification of the renal enzyme, resulting in an 825-fold renin enrichment in a single step. Two antibodies were able to distinguish between active and inactive renin and enabled concomitant separation and purification of the two enzyme forms in various biological fluids. Monoclonal antibodies also stained human and monkey renal renin when indirect immunofluorescence and peroxidase-antiperoxidase techniques were used. A highly sensitive radioimDr. Atlas is with the

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

New monoclonal antibodies directed against human renin. Powerful tools for the investigation of the renin system.

Galen¹,

Devaux²,

Atlas³

et al. 1984

J. Clin. Invest.

134

View full text Add to dashboard Cite

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“…Schnell e Wood (1995) obtiveram como valores normais para a FC em Callithrix jacchus que não foram submetidos ao estresse de 153±4 bpm durante o dia e 220±6 bpm durante a noite. Michel et al (1984) observaram que a FC era menor em saguis-de-tufo-preto livres do que em saguis-de-tufo-preto sob contenção, que foi de 348±51 bpm. Nossa pesquisa obteve FC média de 264±74 bpm, variando entre 112 e 439 bpm.…”

Section: Discussionunclassified

Valores eletrocardiográficos em saguis-de-tufo-preto (Callithrix penicillata)

et al. 2013

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Pesq. Vet. Bras. 33(7):937-941, julho 2013 937 RESUMO.-O conhecimento de parâmetros cardíacos em animais modelo experimentais é essencial para a investigação médica comparativa. Nosso estudo teve como objetivo estabelecer parâmetros eletrocardiográficos e valores de referência para saguis-de-tufo-preto (Callithrix penicillata). Dezenove saguis-de-tufo-preto adultos saudáveis foram utilizados. Uma combinação de tiletamina e zolazepam foi empregada para contenção química antes da realização do exame eletrocardiográfico (ECG Knowledge of cardiac parameters in experimental animal models is essential for comparative medical research. Our study aimed to establish electrocardiographic parameters and reference values for marmosets (Callithrix penicillata). Nineteen healthy adult marmosets were used. A combination of tiletamine and zolazepam was used for chemical restrained before performing electrocardiography (ECG). A computerized ECG machine was used. Values of the ECG parameters were a mean heart rate of 264±74 beats/min, a variation MCA between 60° and -90°, a mean P wave duration of 34±6ms and amplitude of 0.132±0.051mV, a mean PR interval duration of 56±11ms, a mean QRS complex duration of 35±7ms and amplitude of 0.273±0.269mV, a mean QT interval duration 130±26ms, ST segment was isoelectric (13 animals) and with elevation (six animals) and a mean T wave amplitude of 0.19±0.083mV and positive. There was no significant difference between males and females. Thus, the electrocardiographic parameters obtained in our study in marmosets can be used as a reference values to in other future researches, providing researchers electrocardiographic parameters that contribute with the literature.INDEX TERMS: Primate, marmoset, Callithrix penicillata, cardiology, electrocardiography.

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“…The animals were acclimatized in individual metabolic cages for 1 day before starting a 24-hour urine collection at 10:00 AM. Food and water intakes, urinary volume, and urinary Na+ and K' concentrations were measured daily for 4 successive days. 20 The urinary concentrating ability of the kidneys was tested by keeping the rats in metabolic cages for 24 hours without any water.…”

Section: Biological Modelmentioning

confidence: 99%

Physiological and immunopathological consequences of active immunization of spontaneously hypertensive and normotensive rats against murine renin.

et al. 1990

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Spontaneously hypertensive Okamoto-strain rats (SHR) and normotensive Wistar-Kyoto (WKY) rats were actively immunizedwith mouse renin to investigate the effect on blood pressure of blocking the renin-angiotensinogen reaction. Ten male SHR and 10 male WKY rats were immunizedwith purified mouse submandibular gland renin. Control rats were immunized with bovine serum albumin. Antirenin antibodies were produced by both SHR andWKY rats, but renin-immunized SHR had higher titers of circulating renin antibodies after three injections. The increase in renin antibody in renin-immunized SHR was associated with a significant drop in blood pressure (tail-cuff method) that became similar to that of theWKY control rats after four injections. The blockade by antirenin immunoglobulins of the renin-angiotensinogen reaction also decreased the blood pressure of normotensive rats. Perfusion of renin-immunized rats with mouse submandibular renin (10,g) in vivo caused no increase in blood pressure. Perfusion of renin-immunized, salt-depleted SHR with converting enzyme inhibitor caused no further decrease in blood pressure but significantly decreased blood pressure in salt-depleted control rats. The presence of circulating renin antibodies was associated with low plasma renin activity (0.31±0.23 ng angiotensin I [Ang I]/ml/hr). Plasma renin activity was unchanged in control animals (13.1±3.9 ng Ang ImlI/hr in control SHR, 13.9±3.2 ng Ang VImlIhr in control WKY rats). Renin antibody-rich serum produced a dose-dependent inhibition of rat renin enzymatic activity in vitro. The chronic blockade of the renin-angiotensinogen reaction in renin-immunized SHR produced an almostcomplete disappearance of Ang 11 (0.8±7fmol/ml; control SHR, 30.6±+15.7 fmol/ml) and a 50%o reduction in urinary aldosterone. Renin immunization was never associated with a detectable loss of sodium after either 10 or 24 weeks. The glomerular filtration rate was not decreased 10 weeks after renin immunization, whereas blood pressure was significantly decreased, plasma renin activity was blocked, and renal plasma flow was increased. The ratio of left ventricular weight to body weight after 24 weeks was significantly below control levels in renin-immunized WKY rats and SHR. Histological examination of the kidney of renin-immunized SHR showed a chronic autoimmune interstitial nephritis characterized by the presence of immunoglobulins, mononuclear cell infiltration, and fibrosis around the juxtaglomerular apparatus. These expenments demonstrate that chronic specific blockade of renin decreases blood pressure in a genetic model of hypertension in which the renin-angiotenisn system is not directly involved. It also offers a model for comparing chronic renin inhibition with other methods of blocking the renin-angiotensin system in the hypertensive rat. (Circulation 1990;81:1899-1910 T he most specific way to block the reninangiotensinogen. The renin-angiotensinogen reacangiotensin system is to inhibit its first and tion can be inhibited by passive administration of rate-limitin...

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Blood pressure effects of renin inhibition by human renin antiserum in normotensive marmosets

Cited by 22 publications

References 0 publications

New monoclonal antibodies directed against human renin. Powerful tools for the investigation of the renin system.

New monoclonal antibodies directed against human renin. Powerful tools for the investigation of the renin system.

Valores eletrocardiográficos em saguis-de-tufo-preto (Callithrix penicillata)

Physiological and immunopathological consequences of active immunization of spontaneously hypertensive and normotensive rats against murine renin.

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