Blood Pressure Lowering Effect of a Pea Protein Hydrolysate in Hypertensive Rats and Humans

Li, Huan; Prairie, Natalie; Udenigwe, Chibuike C.; Adebiyi, Abayomi P.; Tappia, Paramjit S.; Aukema, Harold M.; Jones, Peter J.H.; Aluko, Rotimi E.

doi:10.1021/jf201911p

Cited by 117 publications

(98 citation statements)

References 45 publications

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“…The reported 46% degree of tryptic hydrolysis of canola protein (Alashi et al 2014) and 19% degree of alcalase hydrolysis of hemp seed protein (Girgih et al 2011) were also higher than that obtained in this study. The low degree of alcalase hydrolysis may be attributed to the low E:S ratio because other studies with higher DH used a higher E:S ratio (Alashi et al 2014;Girgih et al 2011;Li et al 2011;Malomo et al 2015;Udenigwe et al 2009). …”

Section: Discussionmentioning

confidence: 98%

In vitro antioxidant and α-amylase inhibitory properties of watermelon seed protein hydrolysates

Arise¹

2016

EEB

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Proteins from watermelon (Citrullus lanatus L.) seed were isolated using acid-induced precipitation method and then hydrolysed using pepsin, trypsin and alcalase. The hydrolysates were investigated for in vitro antioxidant and α-amylase inhibitory properties. The yield of peptic hydrolysis (68.9 ± 1.0%) was significantly higher than of tryptic (41.4 ± 1.1%) and alcalase (38.5 ± 0.5%) hydrolysis. Peptic hydrolysate showed the highest radical-scavenging ability whereas tryptic hydrolysate gave the highest reducing ability. In a concentrationdependent manner, the hydrolysates demonstrated potent α-amylase inhibitory ability with alcalase and tryptic hydrolysates exhibiting 86.0 ± 3.9 and 83.0 ± 3.5% α-amylase inhibition respectively (IC 50 0.149 to 0.234 mg mL -1). Kinetic analysis revealed that the three enzyme hydrolysates inhibited α-amylase activity via a non-competitive inhibition mechanism. The results therefore indicate that these multidirectional bioactivities of watermelon seed protein hydrolysates may serve as useful tools in the formulation of antidiabetic agents.

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Section: Discussionmentioning

confidence: 98%

In vitro antioxidant and α-amylase inhibitory properties of watermelon seed protein hydrolysates

Arise¹

2016

EEB

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“…Pea protein hydrolysate was made by thermolysin action followed by membrane filtration. Oral administration of the pea protein hydrolysate, containing <3 kDa peptides, to SHR at doses of 100 and 200 mg/kg body weight led to a lowering of SBP, with a maximum reduction of 19 mmHg at 4 h. In contrast, orally administered unhydrolysed pea protein isolate had no blood pressure reducing effect in SHR, suggesting that thermolysin hydrolysis may have been responsible for releasing bioactive peptides from the native protein [143]. Pea protein peptides from in vitro gastrointestinal digestion were observed to absorb poorly with in vitro model and the hypotensive effect was tested with intravenous administration [144].…”

Section: Legume Derived Peptidesmentioning

confidence: 96%

“…The first direct renin-inhibitor, aliskiren, is currently under phase III trials to evaluate its potential as an antihypertensive drug [176]. Thermolysin digest of pea protein decreased remarkably the renal expression of renin mRNA levels in vivo and lowered plasma levels of angiotensin II, thus the reduction in blood pressure in SHR and human subjects was likely due to the effects on the renal angiotensin system [143]. Peaderived peptides Ile-Arg, Lys-Phe and Glu-Phe showed strong inhibitions in vitro studies of ACE and renin [133] as well as ACE-inhibitory peptide fractions from flaxseed protein hydrolysates possessed inhibition also against renin [126,177,178].…”

Section: Health Benefitsmentioning

confidence: 99%

Antihypertensive Properties of Plant Protein Derived Peptides

Pihlanto¹,

Mäkinen²

2013

Bioactive Food Peptides in Health and Disease

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“…17,21,67,68 Peptides from plants such as pea, spinach; macroalgae and mushrooms have showed significant antihypertensive activities. 22,23,25 Cereals such as wheat, buckwheat, soybean have also been used for production of antihypertensive peptides. 57,58,60 Further studies are needed to isolate and identify the bioactive peptides with ACE-inhibitory activities from other dietary food sources.…”

Section: Discussionmentioning

confidence: 99%

“…22 Pea protein isolate, hydrolyzed with alcalase, have proven inhibitory against ACE, renin, and calmodulin-dependent phosphodiesterase 1 (CaMPDE). 23 Four peptides of sequence: ITP IIP GQY STYQT have been isolated by protease enzyme digestion of sweet potato protein. ITP peptide was found to be the most potent ACE inhibitor as concluded by in vivo study on rats.…”

mentioning

confidence: 99%

Antihypertensive Peptides Derived From Food Sources

Balgir¹

2016

MOJFPT

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Abbreviations: ACE, angiotensin-converting enzyme; RAS, renin-angiotensin system; ENOS, endothelial nitric oxide synthase; NO, nitric oxide; LAB, lactic acid bacteria; SHRs, spontaneously hypertensive rats; ECE, endothelin-1 and endothelin converting enzyme; ET, endothelin; MRW, met-Arg-Trp; FPI, flaxseed protein isolate; RPI, rapeseed protein isolate; BSFP, black-bone silky fowl muscle peptides; DBP, diastolic blood pressure; IER, inhibitory efficiency ratio; AHPM, antihypertensive peptide multimer IntroductionHigh blood pressure or hypertension is a serious medical condition, caused due to a high flow of blood through the blood vessels with a force greater than normal. It strains the heart muscles and damages the blood vessels leading to stroke and finally death, if untreated. 1Untreated hypertension is usually associated with a progressive rise in blood pressure.2 It is the most common cause of morbidity and chronic metabolic disorders in the world. It is estimated to affect billions of individuals worldwide affecting 25% of most adult populations. 3,4 Hypertension as been called a silent killer as it has been implicated as the underlying cause for development of haemorrhagic stroke, cognitive decline, myocardial infarction, heart failure, chronic kidney disease and premature death. 5Hypertension may be classified as essential or secondary. Essential hypertension is the term for high blood pressure with no known etiological cause. Essential hypertension is caused by many factors:ii. lifestyle and environmental influences, andiii. disturbances in vascular structure and neuro-humoral control mechanisms. 5It accounts for about 95% of cases. Secondary hypertension is the term for high blood pressure associated with a known aetiology such as kidney disease, tumours or birth control pills. At present, it is widely accepted that approximately 30-50% of cases of hypertension can arise from genetic susceptibility. Monogenic forms of hypertension provide a unique opportunity to study the effects of single gene and identifying pathways and mechanisms leading to blood pressure elevation. 6 A number of pathways have been involved in the causation of hypertension amongst humans. Prominent among them are the pathways for fluid and electrolyte balance; the reninangiotensin system (RAS), the kinin-kallikrein system, the neutral endopeptidase system and the endothelin-converting enzyme system. Thus in clinical practice, vasodilators, diuretics, calcium channel blockers, angiotensin II receptor blockers and ACE inhibitors have been normally used as drugs.7 Most drugs target one or the other pathways to control hypertension. The response to drugs is varied and thus in non-responders may lead to metabolic disorders like diabetes, vascular blockage etc.Due to a high prevalence of hypertension among human population, a multipronged approach including changes in life-style, dietary approaches and pharmacological treatments is the need of the hour. Peptides of food origin have been reported to play an important prophylactic role in t...

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Blood Pressure Lowering Effect of a Pea Protein Hydrolysate in Hypertensive Rats and Humans

Cited by 117 publications

References 45 publications

In vitro antioxidant and α-amylase inhibitory properties of watermelon seed protein hydrolysates

In vitro antioxidant and α-amylase inhibitory properties of watermelon seed protein hydrolysates

Antihypertensive Properties of Plant Protein Derived Peptides

Antihypertensive Peptides Derived From Food Sources

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