Blood RNA Integrity is a Direct and Simple Reporter of Radiation Exposure and Prognosis: A Pilot Study

Alkhalil, Abdulnaser; Clifford, John L.; Ball, Robert L.; Day, Anna; Chan, Rosanna; Carney, Bonnie C; Miller, Stacy Ann; Campbell, Ross; Kumar, Raina; Gautam, Aarti; Hammamieh, Rasha; Moffatt, Lauren T.; Shupp, Jeffrey W.

doi:10.1667/rr15527.1

Cited by 2 publications

(3 citation statements)

References 47 publications

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“…The high levels of radiation dose exposures were associated with a poor prognosis and were generally lethal. In agreement with these clinical observations, recent work from our lab established a correlation between the RNA integrity number (RIN) retrieved from blood samples and radiation dose in mice [ 50 ]. Late time points (>7 days) after exposure to high doses (>5 Gy) yielded lower RNA quantity and quality in concordance with the reported leukocytopenia and recovered only in lower dose exposures.…”

Section: Discussionmentioning

confidence: 64%

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Transcriptomics of Wet Skin Biopsies Predict Early Radiation-Induced Hematological Damage in a Mouse Model

Alkhalil

Clifford

Miller

et al. 2022

Genes

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The lack of an easy and fast radiation-exposure testing method with a dosimetric ability complicates triage and treatment in response to a nuclear detonation, radioactive material release, or clandestine exposure. The potential of transcriptomics in radiation diagnosis and prognosis were assessed here using wet skin (blood/skin) biopsies obtained at hour 2 and days 4, 7, 21, and 28 from a mouse radiation model. Analysis of significantly differentially transcribed genes (SDTG; p ≤ 0.05 and FC ≥ 2) during the first post-exposure week identified the glycoprotein 6 (GP-VI) signaling, the dendritic cell maturation, and the intrinsic prothrombin activation pathways as the top modulated pathways with stable inactivation after lethal exposures (20 Gy) and intermittent activation after sublethal (1, 3, 6 Gy) exposure time points (TPs). Interestingly, these pathways were inactivated in the late TPs after sublethal exposure in concordance with a delayed deleterious effect. Modulated transcription of a variety of collagen types, laminin, and peptidase genes underlay the modulated functions of these hematologically important pathways. Several other SDTGs related to platelet and leukocyte development and functions were identified. These results outlined genetic determinants that were crucial to clinically documented radiation-induced hematological and skin damage with potential countermeasure applications.

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Section: Discussionmentioning

confidence: 64%

“…Hematological perturbation after radiation exposure has been documented in humans and other species [ 48 , 49 , 50 ]. A damage-compensating enhanced proliferation of stem cells dominated responses at low IR doses (≈ 0.2–0.3 Gy) to replenish irreparably affected hematocytes.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomics of Wet Skin Biopsies Predict Early Radiation-Induced Hematological Damage in a Mouse Model

Alkhalil

Clifford

Miller

et al. 2022

Genes

Self Cite

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“…The effectiveness of traditional antibiotics is decreasing due to increasing bacterial resistance. Novel antibacterial antibodies, probiotics, antimicrobial peptides, chitosan, nanomaterials and silver-impregnated foam dressings have been evaluated as possible alternatives to traditional antibiotics in wound infection models [16,21,22,45,47,64,74]. Interestingly, a new therapeutic strategy based on bacterial interference was developed in an experimental rodent scald model [21].…”

Section: Antimicrobial Strategiesmentioning

confidence: 99%

Recent Advances in Experimental Burn Models

Hao

Nourbakhsh

2021

Biology

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Experimental burn models are essential tools for simulating human burn injuries and exploring the consequences of burns or new treatment strategies. Unlike clinical studies, experimental models allow a direct comparison of different aspects of burns under controlled conditions and thereby provide relevant information on the molecular mechanisms of tissue damage and wound healing, as well as potential therapeutic targets. While most comparative burn studies are performed in animal models, a few human or humanized models have been successfully employed to study local events at the injury site. However, the consensus between animal and human studies regarding the cellular and molecular nature of systemic inflammatory response syndrome (SIRS), scarring, and neovascularization is limited. The many interspecies differences prohibit the outcomes of animal model studies from being fully translated into the human system. Thus, the development of more targeted, individualized treatments for burn injuries remains a major challenge in this field. This review focuses on the latest progress in experimental burn models achieved since 2016, and summarizes the outcomes regarding potential methodological improvements, assessments of molecular responses to injury, and therapeutic advances.

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Blood RNA Integrity is a Direct and Simple Reporter of Radiation Exposure and Prognosis: A Pilot Study

Cited by 2 publications

References 47 publications

Transcriptomics of Wet Skin Biopsies Predict Early Radiation-Induced Hematological Damage in a Mouse Model

Transcriptomics of Wet Skin Biopsies Predict Early Radiation-Induced Hematological Damage in a Mouse Model

Recent Advances in Experimental Burn Models

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