Blood Sample Collection and Handling

Lew, Kristi

doi:10.1016/b978-0-12-381373-2.00068-5

Cited by 4 publications

(4 citation statements)

References 12 publications

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“…Remarkably, a specific set of membrane and cytoskeletal proteins (SLC4A1, RHAG, DSC1, DSP, and JUP), which play crucial roles in maintaining the stability and structure of the cell membrane and the shape of a cell, were observed to be dramatically decreased in DBS. Since the blood cell is composed of 99% red blood cells (RBCs) and only 1% white blood cells and platelets, 41 we attribute the majority of membrane proteins to erythrocytes. As such, we found that the abundance of five erythrocyte membrane skeletal proteins including αand βspectrin (SPTA1 and SPTB), tropomodulin 1 (TMOD1), αadducin (ADD1), and ankyrin (ANK1), 32 albeit not statistically significant, was concordantly decreased by more than 1.2-fold in DBS relative to that in whole blood samples (Figure S3; Table S7).…”

Section: ■ Discussionmentioning

confidence: 99%

Temporal Assessment of Protein Stability in Dried Blood Spots

Sun,

Huang,

Wen

et al. 2024

J. Proteome Res.

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The use of protein biomarkers in blood for clinical settings is limited by the cost and accessibility of traditional venipuncture sampling. The dried blood spot (DBS) technique offers a less invasive and more accessible alternative. However, protein stability in DBS has not been well evaluated. Herein, we deployed a quantitative LC−MS/MS system to construct proteomic atlases of whole blood, DBSs, plasma, and blood cells. Approximately 4% of detected proteins' abundance was significantly altered during blood drying into blood spots, with overwhelming disturbances in cytoplasmic fraction. We also reported a novel finding suggesting a decrease in the level of membrane/cytoskeletal proteins (SLC4A1, RHAG, DSC1, DSP, and JUP) and an increase in the level of proteins (ATG3, SEC14L4, and NRBP1) related to intracellular trafficking. Furthermore, we identified 19 temporally dynamic proteins in DBS samples stored at room temperature for up to 6 months. There were three declined cytoskeleton-related proteins (RDX, SH3BGRL3, and MYH9) and four elevated proteins (XPO7, RAN, SLC2A1, and SLC29A1) involved in cytoplasmic transport as representatives. The instability was governed predominantly by hydrophilic proteins and enhanced significantly with an increasing storage time. Our analyses provide comprehensive knowledge of both short-and long-term storage stability of DBS proteins, forming the foundation for the widespread use of DBS in clinical proteomics and other analytical applications.

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Section: ■ Discussionmentioning

confidence: 99%

Temporal Assessment of Protein Stability in Dried Blood Spots

Sun,

Huang,

Wen

et al. 2024

J. Proteome Res.

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“…Changes in LDH levels in blood bags stored in cooler boxes during the transportation process can increase in almost all conditions of organ or tissue damage or when cell destruction occurs. Cell damage and hemolysis that occur can also be seen through increased levels of various substances in the plasma that were previously contained by erythrocytes [18]. Changes in the shape of erythrocytes can be caused by reduced ATP in erythrocytes.…”

Section: Characteristics Of Research Subjectsmentioning

confidence: 99%

Untitled

2023

J. Med. Chem. Sci.

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Blood transfusion service is a health service effort that includes planning, mobilizing, and maintaining blood donors, supplying blood, distributing blood, and medical acts of giving blood to patients to cure diseases and restore health. Blood for transfusion is transported to the hospital using a cooler box without any equipment. This condition is very likely to reduce blood quality during the transportation process due to vibration during transportation. Indications of damage can be seen in changes in the color of blood plasma. The type of research used in this study was a quasiexperimental design with a pretest-posttest with the control group. This study was divided into 3 groups: Group 1, initial observation without treatment, and final observation in groups 2 and 3. Inclusion criteria were: The age range of 17 to 25 years old, the minimum body weight of 45 kilograms, the minimum HB of 12.5 gr/dl, systolic blood pressure of 110-160 mmHg and diastolic 70-90 mmHg, not currently taking medication within three days, and women not menstruating, pregnant, or breastfeeding. Examination of hemoglobin and erythrocytes was done using a hematology analyzer and LDH examination using spectrophotometry. The results showed that the number of erythrocytes after treatment (posttest) was different according to the treatment group (p = 0.042). Treatment in the 5 Hz vibration group reduced the lowest erythrocyte levels, namely to 4,220,000 /mm 3 , compared to the control group and 10 Hz vibration. Hemoglobin levels after treatment (posttest) differed according to the treatment group (p = 0.015); treatment at 10 Hz vibration reduced the lowest hemoglobin level, namely 10.77 g/dL, compared to the control group and the 5 Hz vibration group. LDH levels after treatment (posttest) differed according to the treatment group (p = 0.000).

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“…Previous studies have been conducted to develop a rubber arm with a fluid system for venipuncture training, aiming to enhance the effectiveness of blood drawing for medical science and nursing students. This innovation can assist students in performing the procedure with confidence by providing a better understanding of the steps involved [ 7,8] . The results showed that 72.…”

Section: Introductionmentioning

confidence: 99%

“…The results showed that 72. 62% of students appreciated learning the procedure using an artificial arm before performing blood collection from patients [ 7] . Synthetic polymers for venipuncture practice offer precise structures and customizable properties.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Development of Venipuncture Practice Rubber Model Based for BCG Economy Framework

Somsuan,

Aluksanasuwan,

Morchang

et al. 2024

ASEAN Sci Tech Rept

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The Bio-Circular-Green (BCG) economy framework, which integrates economic, social, and environmental aspects for waste management, holds great promise for fostering growth across various sectors in Thailand. Venipuncture, a critical medical procedure, can lead to complications if not performed correctly. This study uses Thailand para latex resources and calcium carbonate extracted from recycled mollusk shells to develop an educational innovation model for venipuncture procedures. Natural rubber latex (NRL) was obtained from Chiang Rai, Thailand. Preserved latex was heated to obtain 60% concentrated latex, then combined with a sulfur solution, and calcium carbonate powder was added in various ratios. Molding used a specialized mold replicating anatomical features of the antecubital fossa, incorporating three prominent veins. The venipuncture practice rubber model was characterized for pH, moisture content, water absorption, tensile strength, and other properties. The model exhibited neutral pH, low moisture content, and notable resistance and elasticity. This study promotes the sustainable utilization of natural latex resources and recycled mollusk shells in developing innovative medical models within the BCG framework. Additional research is required to enhance the human friendliness and realism of the rubber material used in the venipuncture practice model.

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Blood Sample Collection and Handling

Cited by 4 publications

References 12 publications

Temporal Assessment of Protein Stability in Dried Blood Spots

Temporal Assessment of Protein Stability in Dried Blood Spots

Untitled

Characterization and Development of Venipuncture Practice Rubber Model Based for BCG Economy Framework

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