“…Most published estimates of the methionine plus cystine requirements of the growing rat and the growing pig have been based on either growth and nitrogen balance results (Byington, Howe & Clark, 1972;Sowers, Stockland & Meade, 1972;Braude & Esnaola, 1973;Stockland, Meade, Wass & Sowers, 1973;Brown & Cline, 1974) or serum and plasma amino acid levels (Byington & Howe, 1972;Keith, Christensen & Owen, 1972;Knipfel, Owen & Christensen, 1972;Keith, Peace, Botting & Hamilton, 1975). There has been very little research (Benevenga, 1974;Finkelstein, 1974) into whether enzymes associated with the methionine metabolic pathway could be utilized in assessing the optimal requirements for methionine plus cystine.…”