Blood serum proteins and the mineralization of bone ground substance

Lipp, W

doi:10.1007/bf00305817

Cited by 16 publications

(4 citation statements)

References 19 publications

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“…There was no difference in the staining pattern between young and old teeth. The histological staining was subjected to the following examination to confirm its immIntunological specificity: ( 1) In place of the labeled antibody, labeled goat or rabbit -yglobulin, which was niot reactive to any rabbit or huiman seruLIn proteins, respectively, was added to the sections.…”

Section: Resultsmentioning

confidence: 99%

“…The incorporation of injected homologous serum albumin into the bone and dentin matrices of rodents has already been demonstrated by other histological techniques. 1,2,8 There were many layers of endogenous serum albumin in the inner region of dentin. To investigate the incorporation pattern of serum albumin chronologically, an exogenous serum albumin, BSA, was injected-into rabbits.…”

Section: Discussionmentioning

confidence: 99%

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Localization of Serum Albumin in Dentin and Enamel

Okamura

1983

J Dent Res

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By means of an immunohistological method, the localization of serum albumin in rabbit dentin and enamel and in human dentin was investigated, in order to obtain more information about the movement of serum albumin in the mineralized portion ofthe tooth. The observations suggest two different paths of serum albumin in tooth mineralization. J Dent Res 62(2): 100-104, February 1983 Introduction.An histological study demonstrated the incorporation of serum albumin into the bone matrix of rats, by means of administering a fluorescein-labeled homologous serum albumin.1 In the matrices of dentin and enamel of rabbits, the incorporation pattern of homologous serum albumin was investigated by an autoradiographic method.2 Immunochemical experiments have been concerned with the presence of serum albumin in the non-collagenous matrices of human dentin and bone.3,4 However, the localization of serum albumin in dentin and enamel has not yet been studied.In the present study, the localization of serum albumin was observed in rabbit dentin and enamel and in human dentin, by an immunohistological method, in order to obtain more information about the movement of serum albumin in the mineralized portion of the tooth. Materials and methods.Preparation of tissue specimens. -The following teeth were examined for the localization of endogenous serum albumin: molar teeth removed from the mandibles of ten male Japanese white rabbits weighing 1.0 ± 0.1 kg; and ten vital human teeth with no caries or pulpitis, which were extracted from patients suffering from periodontitis and ranging in age from 28 to 50 yr. The rabbit teeth were immediately fixed in 4% paraformaldehyde/0. I M phosphate buffer (pH 7.3) at 40C for 16 h. Human teeth were fixed at 4°C for two to three d in the same fixative, after a small burr hole reaching the pulp had been made at the height of the cervix of each tooth. The human teeth were then cut at the cervix, and the coronal segments were used. All specimens were rinsed in 0.01 M phosphate-buffered saline (PBS, pH 7.2) at 40C for 16 h. The rabbit teeth were then demineralized in 10% ethylenediaminetetraacetic acid disodium-salt (pH 7.3) at 40C for ten d, and the human teeth were demineralized for one mo. After being washed again, they were dehydrated in ethanol at 40C for three h or three d, and were immersed in xylene at 40C for 90 min. The specimens were embedded in paraffin at 570C for 90 min, and were then cut in 7-to-14-,um thicknesses.Localization of endogenous serum albumin. -To visualize endogenous serum albumin, the direct fluorescent antibody technique was applied. Fluorescein isothiocyanate (FITC)-labeled goat antibody to rabbit serum albumin* and FITC-labeled rabbit antibody to human serum albumint 100 were obtained from commercial sources. Immunological specificity of each antibody to rabbit or human serum, respectively, was confirmed by immunoelectrophoresis.5The labeled antibody solution was re-purified, when necessary, using a DEAE-cellulose column.6 The fractions showing good FITC/protein ratios (1...

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Localization of Serum Albumin in Dentin and Enamel

Okamura

1983

J Dent Res

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“…It is well known that bone mineralisation is related to non-collagenous proteins of bone matrix and that albumin coming from plasma is one of these proteins. [15][16][17][18][19][20][21][22] In addition, albumin reduces the phagocytic recognition of bone mineral by macrophages. 23 By contrast, the low albumin concentration in aged people and lower concentration in patients with femoral neck fractures (even though in both cases the values were non-significant) suggests the possibility of the opposite nutritional condition in such subjects.…”

Section: Conceptual Implicationsmentioning

confidence: 99%

Raised serum albumin in hip osteoarthrosis: a comparative study in women of some blood chemical parameters in aging and in cases of femoral neck fractures, osteoporotic vertebral crush fractures, and hip osteoarthrosis.

Rapin¹,

Lagier²

1988

Annals of the Rheumatic Diseases

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“…Albumin is the main protein responsible for calcium binding in plasma [21]. In experimental rickets, serum protein is not incorporated into the uncalcified rachitic rat osteoid, but its uptake begins concomitantly with the onset of mineralization during the healing period [22]. Incorporation of albumin into the rat bone matrix is reduced when growth is retarded [23].…”

Section: CImentioning

confidence: 99%

Incorporation of serum albumin into the developing dentine and enamel matrix in the rabbit incisor

Kinoshita

1979

Calcif Tissue Int

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Rabbit serum albumin labeled with 125I was injected into rabbits (650-870 g). The incorporation pattern of the labeled albumin into the developing dentine and enamel matrix was studied 1 h, 6 h, and 3 days after the injection using autoradiography. Silver grains were observed over the predentine 1 h after the injection. They were found over the dentine matrix adjacent to the predentine-dentine junction at 6 h. The silver grain band was concentrated over the internal dentine matrix at 3 days. However, no appreciable amount of the silver grains could be seen over the enamel matrix at any time after the injection. It is possible that there might be some physiological barrier between the extravascular fluid and the enamel matrix. The role of the albumin in dentine is discussed.

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Blood serum proteins and the mineralization of bone ground substance

Cited by 16 publications

References 19 publications

Localization of Serum Albumin in Dentin and Enamel

Localization of Serum Albumin in Dentin and Enamel

Raised serum albumin in hip osteoarthrosis: a comparative study in women of some blood chemical parameters in aging and in cases of femoral neck fractures, osteoporotic vertebral crush fractures, and hip osteoarthrosis.

Incorporation of serum albumin into the developing dentine and enamel matrix in the rabbit incisor

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