Blood Substitutes: 1995 in the Literature

Winslow, Robert M.

doi:10.1007/978-1-4612-4114-0_1

Cited by 9 publications

(18 citation statements)

References 67 publications

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“…Various types of haemoglobin-based oxygen carriers have been developed as transfusion alternatives, including intramolecular cross-linked, polymerised and polymer-conjugated haemoglobins 16. However, the cell-free structures of these chemically modified haemoglobin-based oxygen carriers resulted in retention of some of the side effects of molecular haemoglobins, such as renal toxicity, vasoconstriction, hypertension, higher incidence of infarction and death, and therefore clinical phase trials with these carriers failed.…”

Section: Discussionmentioning

confidence: 99%

Immediate effects of systemic administration of normal and high O₂-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model

et al. 2020

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BackgroundHaemoglobin vesicles (HbVs) are red blood cell (RBC) substitutes with a phospholipid bilayer membrane and a polyethylene modified surface (diameter=250 nm; P50=28 Torr). They can be preserved for years and can be used in patients of all blood types without the risk of infection. Their oxygen affinity can be modified by changing the allosteric effectors.MethodsLeft pneumonectomy was performed under mechanical ventilation on rats, followed by rapid exsanguination of ~30% of the total circulating blood volume. Rat RBCs shed in 5% human serum albumin (HSA) solution (rat RBC), HbV with high oxygen affinity in 5% albumin solution (low-P50 HbV, P50=9 Torr), normal HbV suspended in 5% albumin (HbV, P50=28 Torr) or 5% HSA was infused for resuscitation. Haemodynamics and oxygenation were evaluated.ResultsSystemic arterial blood pressure significantly decreased after exsanguination and increased after each infusion. In the HbV, low-P50 HbV and rat RBC groups, all rats were liberated from mechanical ventilation and blood pressure was stabilised, whereas 50% of the rats in the HSA group died within 1 hour after weaning from mechanical ventilation. The PaO2 in arterial blood for 1 hour after liberation from mechanical ventilation in the rat RBC, HbV and low-P50 HbV groups was 59.4±12.5, 58.3±10.1 and 70.5±14.5 mm Hg, respectively. The PaO2 in the low-P50 HbV group was significantly higher than those in the rat RBC and HbV groups (p=0.05 for both). Serum lactate elevations due to hypoxic damage were minimised by HbV, low-P50 HbV as well as rat RBCs.ConclusionsThe oxygen-carrying ability of HbV was comparable to that of rat RBCs, even under impaired lung function after pneumonectomy. HbVs with high oxygen affinity may have more beneficial effects on oxygenation in pulmonary resection.

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Section: Discussionmentioning

confidence: 99%

Immediate effects of systemic administration of normal and high O₂-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model

et al. 2020

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“…Increased demand for blood transfusions in emergency and surgical settings has led to augmented need for red blood cell (RBC) substitutes. Different types of oxygen-carrying blood substitutes are thus being investigated [1][2][3]. One of these products is the nanobiotechnology-based polyhemoglobin (PolyHb).…”

Section: Introductionmentioning

confidence: 99%

Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports Both Oxygen and Carbon Dioxide and Also Acts as an Antioxidant

Bian

Rong

Chang

2011

Artificial Cells, Blood Substitutes, and Biotechnology

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by venous blood. The remaining 93% of CO 2 diffuse into RBCs where the gas is either bound to hemoglobin in the carbamate form, or is converted by RBC enzyme carbonic anhydrase (CA) into carbonic acid, which in turn dissociates into an H ϩ ion and an HCO 3 Ϫ ion [8, 9]. The conversion of approximately 70% of CO 2 to HCO 3 Ϫ ions in the circulation serves two important physiological functions. Firstly, it provides a more effi cient means of transport of CO 2 from tissue cells to the lungs. Secondly, the HCO 3 Ϫ ions produced act in a buffer system for metabolic acids. In the absence of a catalyst, the conversion of CO 2 to H ϩ and HCO 3 Ϫ is a very slow reaction. Thus, CO 2 and carbonic acid exist in the blood in a ratio of 400:1, and the proportion of CO 2 to HCO 3 Ϫ is 20:1 [10]. The hydration reaction of CO 2 would therefore occur much too slowly to be of physiological importance were it not for the presence of the zinc-containing metalloenzyme carbonic anhydrase (CA) in the RBCs [11, 12]. CA is involved in the crucial process of CO 2 transport by catalyzing the reversible hydration of CO 2 to carbonic acid, H ϩ and HCO 3 Ϫ , thus playing a fundamental role in the maintenance of acid-base balance of the body.While antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT) have been crosslinked with PolyHb to produce oxygen carriers that can confer antioxidant properties [13], CA has not been incorporated into a PolyHb-enzyme system in suffi cient amount for the transport of CO 2 . The aim of this study is thus to prepare

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“…Some of these fluids, which contain ultra‐pure human or bovine Hb, are currently under clinical trials. They have the potential to use these fluids in the treatment of anemia, stroke, myocardial infection, and cancer therapy 5‐7 . Hb purification or detection can be achieved by using complex centrifugation, filtration, and chemical (such as toluene and pH treatment), physical (homogenization, sonication, etc.…”

Section: Introductionmentioning

confidence: 99%

“…), chromatographic methods, or emissions sensor based systems 8,9 . However, even if the end product contains only a small amount of phospholipids, stroma residues, or other proteins, it may cause undesirable reactions such as anaphylactic reactions, immune reactions, renal malfunction, and vascular coagulation 2,7 . It can also lead to fatal consequences such as leakage of Hb from red blood cells and its uncontrolled flow to the vascular system, systemic vascular constrictions, decreases in blood volume and flow rate, increased release of immune system protectors, and loss of platelet inactivation that can cause clots in the body 10,11 .…”

Section: Introductionmentioning

confidence: 99%

Recognition of human hemoglobin with macromolecularly imprinted polymeric nanoparticles using non‐covalent interactions

Aylaz

Andaç

Deni̇zli̇

et al. 2021

J of Molecular Recognition

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Hemoglobin (Hb) is the most abundant protein in the blood. It is vital for the living as oxygen carriers. Some of the very pure Hb‐containing biological fluids are currently under clinical trial. However, the removal and purification of Hb from the blood are quite difficult, especially when it is at a low concentration level. In this study, the molecularly imprinted polymeric nanoparticles (MIPNs) were prepared using N‐methacryloyl‐histidine methyl ester (MAH) by mini‐emulsion polymerization technique for specific binding of human hemoglobin (HHb). MIPNs in monosize form have a size of 152 ± 4 nm. They also have a high binding capacity (32.33 mg/g) of HHb. MIPNs retain 84% of the re‐binding capacity for HHb after 10 cycles. The nanoparticles have 16 and 5 times higher binding capacity of HHb, respectively, in the presence of bovine serum albumin and lysozyme. Thanks to their high binding capacity and selectivity, MIPNs will allow them to be detected selectively for different target molecules. According to molecular docking, the main binding forces depend on hydrogen bonds and Van der Waals forces in the interaction within 5 Å around MAH molecule are observed through the amino acid residues of HHb at β1 and β2 subunit. The statistical mechanical analysis of docking showed that the free energy (ΔG) is −2732.14 kcal/mol, which indicates the interaction between MAH and HHb is energetically favorable at 298.15°K.

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Blood Substitutes: 1995 in the Literature

Cited by 9 publications

References 67 publications

Immediate effects of systemic administration of normal and high O₂-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model

Immediate effects of systemic administration of normal and high O₂-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model

Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports Both Oxygen and Carbon Dioxide and Also Acts as an Antioxidant

Recognition of human hemoglobin with macromolecularly imprinted polymeric nanoparticles using non‐covalent interactions

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Blood Substitutes: 1995 in the Literature

Cited by 9 publications

References 67 publications

Immediate effects of systemic administration of normal and high O2-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model

Immediate effects of systemic administration of normal and high O2-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model

Polyhemoglobin-superoxide Dismutase-catalase-carbonic Anhydrase: A Novel Biotechnology-based Blood Substitute that Transports Both Oxygen and Carbon Dioxide and Also Acts as an Antioxidant

Recognition of human hemoglobin with macromolecularly imprinted polymeric nanoparticles using non‐covalent interactions

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Immediate effects of systemic administration of normal and high O₂-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model

Immediate effects of systemic administration of normal and high O₂-affinity haemoglobin vesicles as a transfusion alternative in a rat pneumonectomy model