Blood Transfusion and Renal Transplantation

Induction of peripheral tolerance to class I major histocompatibility complex (MHC) alloantigens in adult mice: transfused class I MHC-incompatible splenocytes veto clonal responses of antigen-reactive Lyt-2+ T cells.

“…The results described here will be discussed in the context of pretransplant transfusion effects (20)(21)(22)(23)(24)(25)(26)(27)(28), the veto cell phenomenon first described by Miller and associates (29)(30)(31)(32)(33)(34)(35)(36), and the concept of peripheral tolerance as opposed to tolerance induced by intrathymic clonal deletion ofantigen-reactive T lymphocytes (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11) to active suppression, but due to clonal paralysis of antigenreactive Lyt-2+ CTIrp-and 11,2-producing Lyt-2+ T cells . Since donor lymphocytes exhibit clonal anergy to recipient class I MHC antigens, yet express in vitro remarkably efficient veto functions, we believe that the reciprocal peripheral tolerance in the adult chimeric mice is caused, and maintained, by veto activity of donor and recipient cells.…”

“…Evaluation of pretransplant transfusion effects have indicated that intravenous confrontation with allogeneic cells may induce specific immunosuppression rather than sensitization (20)(21)(22)(23)(24)(25)(26)(27)(28) Miller, Bevan, pioneered the veto cell concept . Accordingly, unlinked to the specificity of their own antigen receptor, veto cells paralyze in vitro the response of T cells reacting to antigens displayed by the veto cells (29-36) .…”

“…Therefore, most strategies attempt first to reduce the pool of immunocompetent peripheral T lymphocytes, and thereafter to induce unresponsiveness by exposing the regenerating (neonatal) immune system to antigen . In the first step, rather invasive techniques are used, such as whole body (15, 16) or total lymphoid irradiation (17), systemic application of antiproliferative drugs (18,19), or a combination of either of these methods.Evaluation of pretransplant transfusion effects have indicated that intravenous confrontation with allogeneic cells may induce specific immunosuppression rather than sensitization (20)(21)(22)(23)(24)(25)(26)(27)(28) Miller, Bevan, pioneered the veto cell concept . Accordingly, unlinked to the specificity of their own antigen receptor, veto cells paralyze in vitro the response of T cells reacting to antigens displayed by the veto cells (29-36) .…”

“…Evaluation of pretransplant transfusion effects have indicated that intravenous confrontation with allogeneic cells may induce specific immunosuppression rather than sensitization (20)(21)(22)(23)(24)(25)(26)(27)(28) . Independently, Miller, Bevan, and associates (29)(30)(31)(32)(33)(34)(35)(36) pioneered the veto cell concept .…”

Effect of Blood Transfusion on Antigen Presentation Function and on Interleukin 2 Generation

“…C3H/HEJ mice were transfused with 0.2 mL of fresh allogeneic blood obtained from C57BL/6 mice; they were killed on days 1, 3, and 7 after BT. A second group of C3H/HEJ mice was transfused with 0.2 mL/d of the same allogeneic blood on three successive days; they were killed on day 7 following the last BT. The antigen presentation function of peritoneal macrophages was measured by utilizing a D10.G4.1 T-helper cell clone; However, in view of the fact that allogeneic blood can cause more rapid growth of tumor, it is essential that further investigations into the nature of BT-induced decrease in tumor resistance be conducted.…”