Blue, green, and red fluorescence emission signatures of green, etiolated, and white leaves

Stober, F.; Lang, Michael; Lichtenthaler, Hartmut K.

doi:10.1016/0034-4257(94)90129-5

Cited by 57 publications

(22 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PSII generates both F685 and F730 fluorescence, while PSI generates a small fraction of F730 fluorescence. The F685/F730 fluorescence ratio has been used as an indicator of plant vigor, and it has an inverse relationship with photosynthetic activity (Stober et al, 1994;Kim et al, 2001b). Representative false-color, fluorescence images of the control, Nep1, and DCMU-treated samples are presented in Figure 4.…”

Section: Chlorophyll a Fluorescence Imaging Suggests A Compensatory Rmentioning

confidence: 99%

Necrosis- and Ethylene-Inducing Peptide from Fusarium oxysporum Induces a Complex Cascade of Transcripts Associated with Signal Transduction and Cell Death in Arabidopsis

et al. 2006

View full text Add to dashboard Cite

Treatment of Arabidopsis (Arabidopsis thaliana) with a necrosis-and ethylene-inducing peptide (Nep1) from Fusarium oxysporum inhibited both root and cotyledon growth and triggered cell death, thereby generating necrotic spots. Nep1-like proteins are produced by divergent microbes, many of which are plant pathogens. Nep1 in the plant was localized to the cell wall and cytosol based on immunolocalization results. The ratio of chlorophyll a fluorescence (F685 nm/F730 nm) significantly decreased after 75-min treatment with Nep1 in comparison to the control. This suggested that a short-term compensation of photosynthesis occurred in response to localized damage to cells. The concentrations of most water-soluble metabolites analyzed were reduced in Arabidopsis seedlings after 6 h of Nep1 treatment, indicating that the integrity of cellular membranes had failed. Microarray results showed that short-term treatment with Nep1 altered expression of numerous genes encoding proteins putatively localized to organelles, especially the chloroplast and mitochondria. Short-term treatment with Nep1 induced multiple classes of genes involved in reactive oxygen species production, signal transduction, ethylene biosynthesis, membrane modification, apoptosis, and stress. Quantitative PCR was used to confirm the induction of genes localized in the chloroplast, mitochondria, and plasma membrane, and genes responsive to calcium/calmodulin complexes, ethylene, jasmonate, ethylene biosynthesis, WRKY, and cell death. The majority of Nep1-induced genes has been associated with general stress responses but has not been critically linked to resistance to plant disease. These results are consistent with Nep1 facilitating cell death as a component of diseases caused by necrotrophic plant pathogens.

show abstract

Section: Chlorophyll a Fluorescence Imaging Suggests A Compensatory Rmentioning

confidence: 99%

Necrosis- and Ethylene-Inducing Peptide from Fusarium oxysporum Induces a Complex Cascade of Transcripts Associated with Signal Transduction and Cell Death in Arabidopsis

et al. 2006

View full text Add to dashboard Cite

show abstract

“…The red and far-red fluorescence bands are due to the Chl complexes PS II and PS I found inside thylakoid membranes of chloroplasts. The blue and green fluorescence emissions of the leaves do not originate in the photosynthetic apparatus but mostly arise from the cell wall of the epidermis and mesophyll layers of the leaves (Broglia 1993, Stober et al 1994, Bongi et al 1994, Lichtenthaler and Schweiger 1998. It has been reported that blue and green fluorescence bands are predominantly emitted by hydroxycinnamic acids (with ferulic acid as most frequently occurring compound) and other plant phenolics covalently bound to the cell walls (Lichtenthaler andSchweiger 1998, Buschmann et al, 2000).…”

Section: Introductionmentioning

confidence: 94%

“…In case of UV-A laser light (355 nm) excitation the radiation is absorbed by the substances i.e. flavonols and cinnamic acid in the epidermal and mesophyll layers which fluoresce blue and green, and cause reduced penetration depth of the exciting light into the leaf (Stober et al 1994). However, the emitted blue-green fluorescence is re-absorbed by the chlorophylls and carotenoids present in the mesophyll, which finally results in a ChlF emission spectra with a modified shape (Stober et al 1994).…”

Section: Growth Characteristics Of Seedlingsmentioning

confidence: 99%

Detection of nickel‐induced stress using laser‐induced fluorescence signatures from leaves of wheat seedlings

Mishra

Gopal

2008

International Journal of Remote Sensing

View full text Add to dashboard Cite

The continuous increase of heavy metal ions in the environment is imposing serious problems in agricultural yield and increases human health threats through accumulation in the food chain. Various studies have shown that heavy metals influence the metabolic processes and pigment concentrations in leaves, and thus affect the laser induced fluorescence (LIF) spectra. Leaf level in vivo LIF spectra using the 488 nm and 355 nm laser lines, fluorescence induction kinetics (FIK) using the 488 nm laser line, and photosynthetic pigments of the control and nickel treated wheat seedlings were measured. The peak parameters of the blue and UVexcited spectral bands were calculated by Gaussian curve fitting. The FIK measured at both the chlorophyll (Chl) fluorescence bands was used to evaluate the fluorescence decrease ratio (R Fd ). The variations in R Fd and fluorescence ratios of intensities, bandwidth and band area, with varying concentrations of nickel, were revealed as promising parameters in determining the health status of wheat seedlings. These leaf level findings can be extended at canopy level in the field using laser based light detection and ranging (LIDAR) systems.

show abstract

“…F690/F740) decreases with increasing leaf Chl content in a curvilinear relationship, which can be used as a good inverse indicator of Chl content changes due to plant growth or stress events (Buschmann, 2007). Finally, the UV excited blue-to-red/NIR fluorescence intensity ratios (F440/F690 and F440/F740) were proposed as indicators of the leaf physiological development (Stober et al, 1994;Meyer et al, 2003), but also as marker of the nutrition availability and stress occurrence (Heisel et al, 1996). The red and NIR fluorescence emissions by Chl a are highly dynamic, being modulated by photochemical and non-photochemical quenching.…”

Section: Chlorophyll Fluorescencementioning

confidence: 99%

Spectral Remote Sensing of the Responses of Soybean Plants to Environmental Stresses

Krezhova¹

2011

Soybean - Genetics and Novel Techniques for Yield Enhancement

View full text Add to dashboard Cite

Blue, green, and red fluorescence emission signatures of green, etiolated, and white leaves

Cited by 57 publications

References 16 publications

Necrosis- and Ethylene-Inducing Peptide from Fusarium oxysporum Induces a Complex Cascade of Transcripts Associated with Signal Transduction and Cell Death in Arabidopsis

Necrosis- and Ethylene-Inducing Peptide from Fusarium oxysporum Induces a Complex Cascade of Transcripts Associated with Signal Transduction and Cell Death in Arabidopsis

Detection of nickel‐induced stress using laser‐induced fluorescence signatures from leaves of wheat seedlings

Spectral Remote Sensing of the Responses of Soybean Plants to Environmental Stresses

Contact Info

Product

Resources

About