Blue honeysuckle fruit (Lonicera caerulea L.) from eastern Russia: phenolic composition, nutritional value and biological activities of its polar extracts

Abstract

In the present work we conducted a comprehensive chemical analysis of blue honeysuckle (Lonicera caerulea) spontaneously growing in eastern Russia. HPLC-DAD-ESI/MS analysis showed cyanidin-3-glucoside as the major constituent among phenolics, while nutritional analysis revealed fibre, protein, calcium and magnesium as the most important macro- and micronutrients, respectively. Fatty acid composition was dominated by polyunsaturated fatty acids, linoleic acid being the most abundant. Furthermore, we evaluated several in vitro biological activities such as antioxidant, antimicrobial, antiproliferative, wound healing and immunomodulatory effects of blue honeysuckle aqueous and ethanolic extracts that are often incorporated in food and nutraceutical preparations. While the fruit extracts were revealed to be potent radical scavengers with significant inhibition of ABTS radical, thus confirming the literature data, their inhibitory effects against microbial pathogens and tumor cell lines were negligible. The fruit aqueous extract did not show toxicity to human fibroblasts, but 24 h treatment with 150-200 μg per mL of extract slightly enhanced the cell migration when tested by scratched wound assay. Worth mentioning was the inhibitory effect displayed by the blue honeysuckle fruit aqueous extract on human lymphocytes.

Lonicera caerulea L. Polyphenols Alleviate Oxidative Stress‐Induced Intestinal Environment Imbalance and Lipopolysaccharide‐Induced Liver Injury in HFD‐Fed Rats by Regulating the Nrf2/HO‐1/NQO1 and MAPK Pathways

“…The LCPs were extracted following the protocols described in the authors’ previous study and stored at −20 °C until use. [ 27 ] l ‐(+)‐ascorbic acid (Vc standard, HPLC ≥ 98.0%) was purchased from Putian Tongchuang Biotechnology Co., Ltd. (Beijing, China).…”

“…Lonicera caerulea L. berries are widely harvested in northeast Russia, China, Japan, and Canada. [ 25 ] Previous studies have reported that polyphenols, including anthocyanins (mainly anthocyanin‐3‐glucoside), phenolic acids (gentisic acid, chlorogenic acid, caffeic acid, and ellagic acid), and flavonoids (catechin, quercetin‐3‐glucoside, and quercetin‐3‐rutinoside), are the main bioactive compounds in L. caerulea L. [ 26 ] These polyphenols exhibit a wide range of physiological functions, [ 27‐28 ] such as reducing cholesterol, [ 29 ] alleviating lung fibrosis, [ 25 ] and moderating non‐alcoholic steatohepatitis. [ 30 ] Previously, we had reported that L. caerulea L. polyphenols (LCPs) can inhibit LPS‐induced liver damage in rats.…”

Technical Report on the notification of berries of Lonicera caerulea L. as a traditional food from a third country pursuant to Article 14 of Regulation (EU) 2015/2283

“…These authors also reported a higher content of C3G in honeysuckle (L. caerulea) than in blueberries (1.77 mg/g vs. 0.02 mg/g). Caprioli et al (2016) reported for haskap berries spontaneously growing in eastern Russia an anthocyanin content of 12.3 mg/g dry weight which corresponded to approximately 1.5 mg/g fresh berries. Caprioli et al (2016) reported for haskap berries spontaneously growing in eastern Russia an anthocyanin content of 12.3 mg/g dry weight which corresponded to approximately 1.5 mg/g fresh berries.…”

Origanum vulgare L. and Rosmarinus officinalis L. Aqueous Extracts in Growing-finishing Pig Nutrition: Effects on Antioxidant Status, Immune Responses, Polyphenolic Content and Sensorial Properties

“…The total phenolic content is expressed as gallic acid equivalents (GAE) per g of dry meat. The results are expressed as the average of three determinations/animal (Caprioli et al, 2016). Protein concentrations of tissue lysates were determined by the Bradford protein assay (Compton & Jones, 1985) to express the GSHPx activity as U/mg of protein (Stables & Gilroy, 2011).…”