Blue Laser Light Counteracts HSV-1 in the SH-SY5Y Neuronal Cell Model of Infection

Zupin, Luisa; Crovella, Sérgio

doi:10.3390/life12010055

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…SH-SY5Y is a human-derived cell of neural tissue origin. In this experiment, seven new iridoid compounds were screened for antiviral activity, and it was found that officinaloside C has good anti-HSV-1 activity on SH-SY5Y cells [ 25 ]. This helps to understand the virulence of officinaloside C in anti-HSV-1-infection, especially herpes encephalitis caused by anti-HSV-1-infection.…”

Section: Discussionmentioning

confidence: 99%

Antiviral Activities of Officinaloside C against Herpes Simplex Virus-1

et al. 2022

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The iridoid compounds in traditional Chinese medicine play a prominent role in their antiviral effects. We previously reported the anti-inflammatory effect of new iridoids from the aerial parts of Morinda officinalis. Nevertheless, several open questions remain to explore the other biological functions of these new iridoid compounds. Herpes simplex virus-1 (HSV-1) is one of the most prevalent pathogens in human beings worldwide and due to limited therapies, mainly with the guanosine analog aciclovir (ACV) and other analogs, the search for new drugs with different modes of action and low toxicity becomes particularly urgent for public health. This study aimed to explore the anti-HSV-1 effects of iridoids from the aerial parts of Morinda officinalis. The dried aerial parts of Morinda officinalis were extracted with 95% ethanol and systematic separation and purification were then carried out by modern column chromatography methods such as silica gel column, RP-ODS column, Sephadex LH-20 gel column, and semi-preparative liquid phase, and the structure of these compounds were identified through the physical and chemical properties and a variety of spectral techniques. The obtained seven new iridoid compounds were screened for antiviral activity on HSV-1 through CCK8 and the cytopathic effect, and then the plaque reduction assay, the anti-fluorescence reporter virus strain replication, and RT-qPCR experiments were carried out to further evaluate the antiviral effect. Seven new iridoid compounds (officinaloside A–G) were identified from the aerial parts of Morinda officinalis, and officinaloside C showed anti-HSV-1 activity. Further functional experiments confirmed that officinaloside C has a significant inhibiting effect on HSV-1 virus plaque formation, viral gene, and protein expression, and fluorescent virus replication. Our findings suggest that officinaloside C has significant inhibitory effects on viral plaque formation, genome replication, and viral protein expression of HSV-1 which implies that officinaloside C exhibits viral activity and may be a promising treatment for HSV-1 infection.

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Section: Discussionmentioning

confidence: 99%

Antiviral Activities of Officinaloside C against Herpes Simplex Virus-1

et al. 2022

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“…Blue light was also described as effective in counteracting HCoV‐229 E, HCoV‐OC43 [ 14 ], SARS‐CoV‐1, MERS‐CoV [ 11 ], HSV‐1 [ 15 , 16 ], and Zika virus [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of near‐infrared and blue laser light on vero E6 cells SARS‐CoV‐2 infection model

Zupin

Clemente²,

Fontana³

et al. 2023

Journal of Biophotonics

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Photobiomodulation therapy (PBMT) employing laser light has been emerging as a safe strategy to challenge viruses. In this study the effect of blue and near‐infrared (NIR) laser light was assessed in an in vitro model of SARS‐CoV‐2 infection. PBMT at blue wavelength inhibited viral amplification when the virus was directly irradiated and then transferred to cell culture and when cells already infected were treated. The NIR wavelength resulted less efficacious showing a minor effect on the reduction of the viral load. The cells receiving the irradiated virus or directly irradiated rescued their viability to level comparable to not treated cells. Virion integrity and antigenicity were preserved after blue and NIR irradiation, suggesting that the PBMT antiviral effect was not correlated to viral lipidic envelope disruption. Our results suggested that PBMT can be considered a valid strategy to counteract SARS‐CoV‐2 infection, at least in vitro.

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Blue Laser Light Counteracts HSV-1 in the SH-SY5Y Neuronal Cell Model of Infection

Cited by 2 publications

References 25 publications

Antiviral Activities of Officinaloside C against Herpes Simplex Virus-1

Antiviral Activities of Officinaloside C against Herpes Simplex Virus-1

Effect of near‐infrared and blue laser light on vero E6 cells SARS‐CoV‐2 infection model

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