Blue light and its receptor white collar complex (FfWCC) regulates mycelial growth and fruiting body development in Flammulina filiformis

Li, Jian; Xu, Chang; Jing, Zhuohan; Li, Xiaoyü; Li, Hui; Chen, Yizhao; Shao, Yanping; Cai, Jianfa; Wang, Bo; Xie, Baogui

doi:10.1016/j.scienta.2022.111623

Cited by 8 publications

(5 citation statements)

References 38 publications

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“…For the stipe and pileus in the elongation stage, the transcription level of FfSpdsSr in the pileus (ELP) was about 4-fold higher than that in the stipe (ELS) ( Figure 4 B). Just as the blue light was usually used to shine F. filiformis in the factory, the two time points (1 h and 3 h, according to the previous study by Li et al [ 18 ]) after blue light irradiation were used for the sample and detection. FfSpdsSr also up-regulated at 1 h and 3 h after blue light irradiation, and its relative expression level at BL-3h was higher than that at BL-1h ( Figure 4 C).…”

Section: Resultsmentioning

confidence: 99%

“…In order to detect the expression of genes in response to light and temperature, on the mycelia of FL19 the following treatments were performed. For the light treatment, blue light with 3 µmoL·m −2 ·s −1 intensity was used to irradiate the mycelia growing on CYM plate for 1 h and 3 h, according to the previous study of Li et al [ 18 ], and the control was that under darkness. For the temperature treatment, four gradients—15 °C (cold stress), 20 °C (low-temperature stress), 25 °C (the optimum temperature, as control), and 30 °C (high-temperature stress)—were used to cultivate F. filiformis mycelia after inoculation on CYM plate, according to the study of Lv et al [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

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Spermidine Synthase and Saccharopine Reductase Have Co-Expression Patterns Both in Basidiomycetes with Fusion Form and Ascomycetes with Separate Form

Yang

Shi

et al. 2023

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Gene fusion is a process through which two or more distinct genes are fused into a single chimeric gene. Unlike most harmful fusion genes in cancer cells, in this study, we first found that spermidine synthetase- (SPDS, catalyst of spermidine biosynthesis) and saccharopine reductase- (SR, catalyst of the penultimate step of lysine biosynthesis) encoding genes form a natural chimeric gene, FfSpdsSr, in Flammulina filiformis. Through the cloning of full-length ORFs in different strains and the analysis of alternative splicing in developmental stages, FfSpdsSr has only one copy and unique transcript encoding chimeric SPDS-SR in F. filiformis. By an orthologous gene search of SpdsSr in more than 80 fungi, we found that the chimeric SpdsSr exists in basidiomycetes, while the two separate Spds and Sr independently exist in ascomycetes, chytridiomycetes, and oomycetes. Further, the transcript level of FfSpdsSr was investigated in different developmental stages and under some common environmental factors and stresses by RT-qPCR. The results showed that FfSpdsSr mainly up-regulated in the elongation stage and pileus development of F. filiformis, as well as under blue light, high temperature, H2O2, and MeJA treatments. Moreover, a total of 15 sets of RNA-Seq data, including 218 samples of Neurospora crassa, were downloaded from the GEO database and used to analyze the expression correlation of NcSpds and NcSr. The results showed that the separate NcSpds and NcSr shared highly similar co-expression patterns in the samples with different strains and different nutritional and environmental condition treatments. The chimeric SpdsSr in basidiomycetes and the co-expression pattern of the Spds and Sr in N. crassa indicate the special link of spermidine and lysine in fungi, which may play an important role in the growth and development of fruiting body and in response to the multiple environmental factors and abiotic stresses.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Spermidine Synthase and Saccharopine Reductase Have Co-Expression Patterns Both in Basidiomycetes with Fusion Form and Ascomycetes with Separate Form

Yang

Shi

et al. 2023

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“…Orthologs of WC-1 and WC-2 are found across the fungal kingdom, including mushroom-forming basidiomycetes (Idnurm et al, 2010; Ohm et al, 2013; Todd et al, 2014). This is expected, as blue light is an essential signal for the development of fruiting bodies in many species, including Coprinopsis cinerea, Flammulina filiformis and Schizophyllum commune (Kamada et al, 2010; Li et al, 2023; Ohm et al, 2013). In S. commune , deletion of either wc-1 or wc-2 prevents mushroom development completely, for which light is an essential signal (Ohm et al, 2013).…”

Section: Introductionmentioning

confidence: 93%

“…Coprinopsis cinerea, Flammulina filiformis and Schizophyllum commune (Kamada et al, 2010;Li et al, 2023;Ohm et al, 2013). In S. commune, deletion of either wc-1 or wc-2 prevents mushroom development completely, for which light is an essential signal (Ohm et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The regulatory network of the White Collar complex during early mushroom development inSchizophyllum commune

Vonk,

van der Poel,

Niemeijer

et al. 2024

Preprint

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Blue light is an important signal for fungal development and is detected by the White Collar complex, which consists of WC-1 and WC-2. Most of our knowledge on this complex is derived from the ascomycete Neurospora crassa, where both WC-1 and WC-2 contain GATA zinc-finger transcription factor domains. In basidiomycetes, WC-1 is truncated and does not contain a transcription factor domain, but both WC-1 and WC-2 are still important for development . In the model mushroom Schizophyllum commune, we show that dimerization of WC-1 and WC-2 happens independent of light, but that induction by light is required for promoter binding by the White Collar complex. Furthermore, the White Collar complex is a promoter of transcription, but binding of the complex alone is not always sufficient to initiate transcription. For its function, the White Collar complex associates directly with the promoters of structural genes involved in mushroom development, like hydrophobins, but also promotes the expression of other transcription factors that play a role in mushroom development.

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“…For example, the blue light recep-tor coding gene Cmwc-1 in C. militaris contributes to pigment precipitation and promotes conidiation [182], the blue light complex transcription factor coding genes Wc-1 and Wc-2 in S. commune inhibit vegetative growth and protect against phototoxicity [183], and blue light receptor coding gene Icwc-1 in Isaria cicadae has the function of regulating genes related to pigment and enzyme synthesis [184]. Similarly, the blue light and its receptor white collar complex coding genes FfWc1 and FfWc2 in F. filiformis can regulate the role of morphogenesis [185]. In addition, Gf.BMR1 from G. frondosa contributes to fruiting body development and pigment accumulation [186], abl-D from L. edodes promotes the formation of brown film in mycelia [187], and Pabs from A. bisporus [188] enhances UV tolerance.…”

Section: Genes Related To Environmental Responsementioning

confidence: 99%

Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects

Li,

Zou,

Bao

et al. 2024

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Functional genes encode various biological functions required for the life activities of organisms. By analyzing the functional genes of edible and medicinal fungi, varieties of edible and medicinal fungi can be improved to enhance their agronomic traits, growth rates, and ability to withstand adversity, thereby increasing yield and quality and promoting industrial development. With the rapid development of functional gene research technology and the publication of many whole-genome sequences of edible and medicinal fungi, genes related to important biological traits have been mined, located, and functionally analyzed. This paper summarizes the advantages and disadvantages of different functional gene research techniques and application examples for edible and medicinal fungi; systematically reviews the research progress of functional genes of edible and medicinal fungi in biological processes such as mating type, mycelium and fruit growth and development, substrate utilization and nutrient transport, environmental response, and the synthesis and regulation of important active substances; and proposes future research directions for functional gene research for edible and medicinal fungi. The overall aim of this study was to provide a valuable reference for further promoting the molecular breeding of edible and medicinal fungi with high yield and quality and to promote the wide application of edible and medicinal fungi products in food, medicine, and industry.

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Blue light and its receptor white collar complex (FfWCC) regulates mycelial growth and fruiting body development in Flammulina filiformis

Cited by 8 publications

References 38 publications

Spermidine Synthase and Saccharopine Reductase Have Co-Expression Patterns Both in Basidiomycetes with Fusion Form and Ascomycetes with Separate Form

Spermidine Synthase and Saccharopine Reductase Have Co-Expression Patterns Both in Basidiomycetes with Fusion Form and Ascomycetes with Separate Form

The regulatory network of the White Collar complex during early mushroom development inSchizophyllum commune

Current Advances in the Functional Genes of Edible and Medicinal Fungi: Research Techniques, Functional Analysis, and Prospects

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