Blue light‐dependent monovalent anion uptake

Quiñones, Miguel Á.; Giráldez, Nuria; Witt, Federico G.; Aparicio, Pedro J.

doi:10.1111/j.1399-3054.1997.tb03453.x

Cited by 11 publications

(3 citation statements)

References 62 publications

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“…The absence of positive correlation between the changes in enzyme activity and the total content of nitrate in a leaf was also noted in other reports [26]. Probably early in leaf development, the higher accumulation of nitrate under BL, as compared to RL, indicates that BL promoted the active input of into a leaf [27]. Amino acids and amides are known to represent the primary stable products of nitrate assimilation.…”

Section: Resultssupporting

confidence: 62%

Effect of Light Quality on Nitrogen Metabolism of Radish Plants

Maevskaya

Bukhov

2005

Russ J Plant Physiol

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The long-term action of blue or red light on nitrogen metabolism was studied in radish ( Raphanus sativus L.) plants. The potential activity of nitrate reductase (NR) in vivo and its maximum activity in vitro , the content of soluble protein and free amino acids were determined in the course of the growth of a third leaf of radish plants. The effect of light quality on NR activity was found to depend significantly on the stage of leaf development. Blue light (BL) stimulated NR activity in leaves, when their areas were about 11-13% of the fully developed leaves. The efficiency of red light (RL) was significantly lower, because the maximum NR activity was observed in the leaves developed to the stage, when their areas were 38-40% of the final one. The comparative analysis of the pool of free amino acids in expanding leaves of BL-or RL-grown plants revealed significant changes in the contents of individual amino acids. Despite a higher accumulation of two amino acids in the leaves of BL-grown plants, namely, Asp (27% as compared to 13-16% in the RL-grown leaves) and Gly (5% against 2.5% in RL-grown leaves), the BL-grown leaves also demonstrated a significant decrease in Ala (10% as compared to 23% in the RL-grown leaves) and some decrease in the amounts of Ser and Gly. The content of soluble protein in a juvenile BL-grown leaf was observed to decrease gradually during leaf development. However, the protein content in the BL-grown leaf was always higher than in the RL-grown leaf of the same age. We concluded that the photoregulatory action of BL on NR activity determined the different rates of nitrogen assimilation in BL-and RL-grown plants. Key words: Raphanus sativus -amino acids -light quality -nitrate reductase -development -soluble proteinAbbreviations : BL-blue light; GS-2/GOGAT-chloroplast glutamine synthetase/glutamine synthase; NR-nitrate reductase; RL-red light.

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Section: Resultssupporting

confidence: 62%

Effect of Light Quality on Nitrogen Metabolism of Radish Plants

Maevskaya

Bukhov

2005

Russ J Plant Physiol

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“…Our results show that a nitrate transport system is absent in mutant stain 23c + after a long incubation period in the light. The participation of light-regulated channels/ transporters seems to be important in regulating physiological processes such as phototropism and stomatal aperture in plants (Cho and Spalding 1996), the osmolarity in halobacteria (Ru¨diger and Oesterheld 1997), and nitrate assimilation in Monoraphidium braunii (Quin˜ones et al 1997) and C. reinhardtii (Quin˜ones et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Chlamydomonas reinhardtii strains expressing nitrate reductase under control of the cabII-1 promoter: isolation of chlorate resistant mutants and identification of new loci for nitrate assimilation

et al. 2005

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The Chlamydomonas reinhardtii strain Tx11-8 is a transgenic alga that bears the nitrate reductase gene (Nia1) under control of the CabII-1 gene promoter (CabII-1-Nia1). Approximately nine copies of the chimeric CabII-1-Nia1 gene were found to be integrated in this strain and to confer a phenotype of chlorate sensitivity in the presence of ammonium. We have used this strain for the isolation of spontaneous chlorate resistant mutants in the presence of ammonium that were found to be defective at loci involved in MoCo metabolism and light-dependent growth in nitrate media. Of a total of 45 mutant strains analyzed first, 44 were affected in the MoCo activity (16 Nit(-), unable to grow in nitrate, and 28 Nit(+), able to grow in nitrate). All the Nit(-) strains lacked MoCo activity. Diploid complementation of Nit(-), MoCo(-) strains with C. reinhardtii MoCo mutants and genetic analysis indicated that some strains were defective at known loci for MoCo biosynthesis, while three strains were defective at two new loci, hereafter named Nit10 and Nit11. The other 28 Nit(+) strains showed almost undetectable MoCo activity or activity was below 20% of the parental strain. Second, only one strain (named 23c(+)) showed MoCo and NR activities comparable to those in the parental strain. Strain 23c(+) seems to be affected in a locus, Nit12, required for growth in nitrate under continuous light. It is proposed that this locus is required for nitrate/chlorate transport activity. In this work, mechanisms of chlorate toxicity are reviewed in the light of our results.

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“…Blue light (BL) is known to specifically affect several physiological processes, such as phototropism ( Jin et al 2001), stomatal aperture (Zeiger 2000), and chloroplast movement ( Jarillo et al 2001) in higher plants; uptake of monovalent anions in microalgae (Quiñones et al 1997); and synthesis of mycosporine‐like amino acids in macroalgae (Franklin et al 2001). One of the effects of BL that has been studied more in detail in macroalgae is the stimulation of red‐light (RL) saturated photosynthesis in brown algae.…”

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confidence: 99%

THE INVOLVEMENT OF A PLASMA MEMBRANE H⁺‐ATPase IN THE BLUE‐LIGHT ENHANCEMENT OF PHOTOSYNTHESIS IN LAMINARIA DIGITATA (PHAEOPHYTA)¹

Klenell

Snoeijs

Pedersén

2002

Journal of Phycology

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Many brown algae, including the kelp Laminaria digitata (Huds.) Lamour., exhibit enhanced photosynthesis when they are given a small amount of blue‐light in addition to a background of saturating red light. This blue light effect is correlated with an increased uptake of carbon. In the present study, we tested the hypothesis that blue light acts by increasing the activity of a plasma membrane H+‐ATPase, thereby promoting an active carbon uptake across the plasma membrane. Photosynthetic carbon uptake was studied in pH‐drift experiments under illumination with red and blue light and using different inhibitors. Vanadate, an inhibitor of plasma membrane H+‐ATPases, had a minor inhibitory effect on carbon uptake rates under saturating red light conditions, but inhibited the blue‐light enhancement by approximately 60%. An inhibitor of external carbonic anhydrase, acetazolamide, decreased the carbon uptake in both red light and in red plus blue light by 48% and 68%, respectively. These results suggest that photosynthetic carbon uptake depends on an external carbonic anhydrase under both red and red plus blue light conditions, and that blue light induces an increased activity of a P‐type H+‐ATPase in the plasma membrane. The proton buffer Tris, which has a buffering capacity similar to vanadate in seawater, had no inhibitory effect on carbon uptake rates neither in red light nor in red plus blue light, showing that the inhibitory effect of vanadate is not caused by its effect as a buffer. The blue‐light enhancement was also abolished by a protein kinase inhibitor (H‐7), suggesting that the transduction of the blue‐light signal involves a protein kinase, which activates the plasma membrane H+‐ATPase by phosphorylation.

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Blue light‐dependent monovalent anion uptake

Cited by 11 publications

References 62 publications

Effect of Light Quality on Nitrogen Metabolism of Radish Plants

Effect of Light Quality on Nitrogen Metabolism of Radish Plants

Chlamydomonas reinhardtii strains expressing nitrate reductase under control of the cabII-1 promoter: isolation of chlorate resistant mutants and identification of new loci for nitrate assimilation

THE INVOLVEMENT OF A PLASMA MEMBRANE H⁺‐ATPase IN THE BLUE‐LIGHT ENHANCEMENT OF PHOTOSYNTHESIS IN LAMINARIA DIGITATA (PHAEOPHYTA)¹

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Blue light‐dependent monovalent anion uptake

Cited by 11 publications

References 62 publications

Effect of Light Quality on Nitrogen Metabolism of Radish Plants

Effect of Light Quality on Nitrogen Metabolism of Radish Plants

Chlamydomonas reinhardtii strains expressing nitrate reductase under control of the cabII-1 promoter: isolation of chlorate resistant mutants and identification of new loci for nitrate assimilation

THE INVOLVEMENT OF A PLASMA MEMBRANE H+‐ATPase IN THE BLUE‐LIGHT ENHANCEMENT OF PHOTOSYNTHESIS IN LAMINARIA DIGITATA (PHAEOPHYTA)1

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THE INVOLVEMENT OF A PLASMA MEMBRANE H⁺‐ATPase IN THE BLUE‐LIGHT ENHANCEMENT OF PHOTOSYNTHESIS IN LAMINARIA DIGITATA (PHAEOPHYTA)¹