Blue-light dependent ROS formation by Arabidopsis cryptochrome-2 may contribute toward its signaling role

Abstract

Cryptochromes are blue-light absorbing flavoproteins with many important signaling roles in plants, including in deetiolation, development, and stress response. They interact with downstream signaling partners such as transcription factors and components of the proteasome, and thereby alter regulation of nuclear gene expression in a light dependent manner. In a prior study, it has also been shown that Arabidopsis cry1 activation by blue light results in direct enzymatic conversion of molecular oxygen (O2) to ROS (reactive oxygen species) in vivo leading to cell death in overexpressing lines. Here we extend these observations to show that Atcry2 is translocated from the cytosol to the nucleus in response to blue light illumination, resulting in nuclear accumulation of ROS in expressing insect cell cultures. These observations suggest that ROS formation may represent a novel means of signaling by Atcry2 distinct from, and perhaps complementary to, the currently known mechanism of light-mediated conformational change.

Blue LED Irradiation Induces Scoparone Production in Wounded Satsuma Mandarin ‘Aoshima Unshu’ and Reduces Fruit Decay during Long-term Storage

“…Phenylalanine ammonia-lyase (PAL) activity is widely known to be activated by wounding stress (Dan et al, 1999). It is increased by blue light irradiation in some plants (Engelsma, 1974) and by blue light induced accumulation of reactive oxygen species in vivo (Jourdan et al, 2015). Considering that PAL activity is increased by stress conditions other than wounding, the scoparone production observed in this study could also have been a reaction to multiple stresses as mentioned above.…”

Introductory Chapter: Characterization and Breeding of Brassica Germplasm

“…These techniques include morphological, cytological, biochemical, and molecular markers. Physiological, biochemical, and molecular genetic techniques have also successfully applied in different plant species [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. This work highlights the current knowledge of the application of physiological and genetic markers in the genus Brassica L. in order to understand its biology, diversity, conservation, and breeding as a basis for further research to develop disease-resistant and more productive crops.…”

Introductory Chapter: Hormonal Regulation in Plant Development and Stress Tolerance

“…GA is also included in the hormonal crosstalk in environmental signals [47]. In conclusion, like the potential use of molecular and genetic markers in crop improvement [48][49][50][51][52][53][54][55][56][57], phytohormones play crucial roles in development and stress tolerance of crops.…”