2021
DOI: 10.1002/adbi.202000307
|View full text |Cite
|
Sign up to set email alerts
|

Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown)

Abstract: that derive from clustered regularly interspaced short palindromic repeats (CRISPR)-based techniques, currently positively reshapes biological studies. [2] Key applications of the specific DNA-recognizing CRISPR/Cas9 systems encompass for example genome editing and transcriptional regulation with high sequence specificity. [2][3][4][5][6] In recent years, these technologies led to major advances in synthetic biology, gene therapy, and gene modification in almost every model organism. Various Cas-variants were … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
8
0

Year Published

2021
2021
2023
2023

Publication Types

Select...
5
1
1

Relationship

0
7

Authors

Journals

citations
Cited by 13 publications
(8 citation statements)
references
References 47 publications
0
8
0
Order By: Relevance
“…Nowadays, optogenetic can be used to treat eye diseases such as color blindness ( Table 1 and Figure 3 ; Cideciyan et al, 2016 ). The researchers sensitized the cells to light by the expression of light genes that encode light-activated channels or pumps in the remaining retinal cells ( Ostrovsky and Kirpichnikov, 2019 ; Blomeier et al, 2021 ; He et al, 2021 ; Kramer et al, 2021 ; Mickoleit et al, 2021 ). Relevant research has achieved good results in recent years.…”
Section: Nervous System-based Clinical Researchmentioning
confidence: 99%
“…Nowadays, optogenetic can be used to treat eye diseases such as color blindness ( Table 1 and Figure 3 ; Cideciyan et al, 2016 ). The researchers sensitized the cells to light by the expression of light genes that encode light-activated channels or pumps in the remaining retinal cells ( Ostrovsky and Kirpichnikov, 2019 ; Blomeier et al, 2021 ; He et al, 2021 ; Kramer et al, 2021 ; Mickoleit et al, 2021 ). Relevant research has achieved good results in recent years.…”
Section: Nervous System-based Clinical Researchmentioning
confidence: 99%
“…We introduced strongLOV, a mutant El222 transcription factor that requires less light for the same level of activity and thus could reduce phototoxicity. As the El222 optogenetic system is extensively used in organisms other than budding yeast such as mammalian cell lines 119 , bacteria 120 , zebrafish 58 , and plants 121 , the new mutation described here ought to be useful for light control experiments in different fields of biology, as well as contribute to further understanding of LOV-domain proteins photochemistry.…”
Section: Discussionmentioning
confidence: 99%
“…24 h before transfection 50,000 cells in 500 ml medium were seeded per well in 24-well plates (Corning). 1,25 mg DNA per well were diluted in 50 ml of OptiMEM and mixed with a PEI/OptiMEM mix [2.5 mL PEI solution (1 mg/ml) in 50 mL OptiMEM] (Blomeier et al, 2021). The ratio between target plasmid (pBES1-pCMVmin::FLUC::tNos) and inducer plasmid (pSV40::PIF4-VP16::pA) was 1:4 (additionally 0,1 mg of normalization plasmid).…”
Section: One-hybrid In Mammalian Cellsmentioning
confidence: 99%
“…100 ml of transfection mix was added to each well in a drop-wise manner. Medium was exchanged after 4 h. 24 h post transfection luminescence was measured with a Berthold Technologies Centro XS 3 LB 960 Microplate luminometer for FLUC and NanoLuc (normalization) (Blomeier et al, 2021). Cells were lysed with 250 ml lysis buffer and 1mM 1,4-Dithiothreitol (DTT) on ice, and afterwards carefully resuspended as previously described (Baaske et al, 2018).…”
Section: One-hybrid In Mammalian Cellsmentioning
confidence: 99%