Blue photosensitizers for aPDT eliminate Aggregatibacter actinomycetemcomitans in the absence of light: An in vitro study

Valle, Luisa Andrade; Lopes, Marcelo Milanda Ribeiro; Zangrando, Mariana Schützer Ragghianti; Sant’Ana, Adriana Campos Passanezi; Greghi, Sebastião Luiz Aguiar; Rezende, Maria Lúcia Rubo de; Damante, Carla Andreotti

doi:10.1016/j.jphotobiol.2019.03.005

Cited by 20 publications

(7 citation statements)

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“…For this reason, in the last decades, the association of LLLT with photosensitizers has also been used to reduce or eliminate bacteria, in the called aPDT. 19 20 28 In this study, the aPDT protocol was the same used by Batalha et al 20 and similar results were obtained. These authors used the same sample of periodontal biofilm to contaminate dental implants in vitro and have a significant reduction in the number of CFUs compared with control group.…”

Section: Discussionsupporting

confidence: 83%

“…The preirradiation time of 1 minute was also defined based on literature. 19,20,28 Although the reduction in the number of CFUs for both cultures with both protocols was quite satisfactory, it has not yet been sufficient to eliminate microorganisms. This indicates that the association of physical and chemical methods, as suggested by Cai et al, 16 can be used for decontamination of dental implant surfaces.…”

Section: Discussionmentioning

confidence: 99%

“… 29 Researchers have preferred TBO for interacting better with lipopolysaccharides from gram-negative bacteria even without a light source, 30 according to some studies reporting the use of the dye alone. 19 28 Besides, TBO has an intense absorption in the region of 620 to 660 nm 29 which justifies selecting the wavelength used in this study. The preirradiation time of 1 minute was also defined based on literature.…”

Section: Discussionmentioning

confidence: 99%

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Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis–related Microorganisms Grown In Vitro

Tonin¹,

Brites²,

Mariano

et al. 2021

Eur J Dent

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Objective Currently, dental implants are a predictable treatment option for oral rehabilitation; however, complications such as peri-implant diseases are increasing every day. Thus, the aim of this study was to verify the efficacy, in vitro, of two protocols against cultures of periodontal biofilm and Staphylococcus aureus. Material and Methods Petri dishes for each of the following groups were used: control groups (C)—plates inoculated with periodontal biofilm (C.B; n = 4) or S. aureus (C.SA; n = 4) without any treatment; laser groups—plates inoculated with periodontal biofilm (low-level laser therapy [LLLT].B; n = 4) or S. aureus (LLLT.SA; n = 4) and treated with LLLT (660 nm, 30 mW, 50 J/cm2, and 47 seconds); antimicrobial photodynamic therapy groups (aPDT)—plates inoculated with periodontal biofilm (aPDT.B; n = 4) or S. aureus (aPDT.SA; n = 4) and treated with aPDT (red laser 660 nm, 30 mW, 50 J/cm2, 47 seconds + toluidine blue O (TBO) 100 µg/mL, and 1 minute). After treatments were performed, the contents of all plates were diluted and seeded for counting colony-forming units (CFUs). Statistical Analysis Results were analyzed with one-way analysis of variance (ANOVA), Tukey’s test, comparison of percentages, and independent t-tests with a 5% significance level. Results Both treatments, LLLT and aPDT, significantly reduced the number of CFUs for the two types of culture, LLLT.B (3.69 × 106 ± 0.20), aPDT.B (2.79 × 106 ± 0.13), LLLT.SA (4.10 × 106 ± 0.12), and aPDT.SA (3.23 × 106 ± 0.10) when compared with control groups C.B (5.18 × 106 ± 0.43) and C.SA (5.81 × 106 ± 0.16; p = 0.000). When treatment groups were compared separately, there was also a statistically significant difference (p = 0.000). None of the protocols were able to eliminate cultured microorganisms. Conclusion The LLLT and aPDT protocols effectively reduced cultures of periodontal biofilm and S. aureus in vitro, with the superiority of aPDT.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis–related Microorganisms Grown In Vitro

Tonin¹,

Brites²,

Mariano

et al. 2021

Eur J Dent

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“…Only one study [20] reported the concentration of photosensitizer used (10 mg). Thus, although the application of aPDT with 10 mg toluidine blue and methylene blue has been reported to be 100% effective in the elimination of A. actinomycetemcomitans in vitro [58], since the concentration of residual photosensitizer after rinsing the pocket with different techniques is not known, the concentration of reactive oxygen species for the elimination of pathogens www.bjbms.org involved in AgP cannot be assessed. Furthermore, the energy used per square millimeter varied in different studies.…”

Section: Discussionmentioning

confidence: 99%

Clinical attachment loss in the use of adjunctive antimicrobial photodynamic therapy in Stages II-IV Grade C molar-incisor periodontitis: A systematic review and meta-analysis

Baghani

Shabestari

Karrabi

2022

Bosn J of Basic Med Sci

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This systematic review and meta-analysis aimed to assess the extent of clinical attachment loss (CAL) as a clinical parameter in the efficacy of antimicrobial photodynamic therapy (aPDT) in non-surgical management of stage II-IV grade C molar-incisor pattern Periodontitis. This review protocol was conducted in accordance with PRISMA statements and is registered in PROSPERO (CRD42022321211). An electronic and manual search was conducted for relevant articles comparing the efficacy of aPDT versus scaling and root planning (SRP) alone or with amoxicillin/metronidazole (AMX/MET) published up until December 2021. The mean clinical attachment loss (CAL), probing depth (PD) reduction, and bleeding on probing (BOP) with a 95% confidence interval (CI) were pooled and compared between the two groups with CAL < and > 7 mm using a random-effect model after 3 and 6 months. To assess the heterogeneity of the findings, the I2 test was applied and Publication bias was evaluated by visual examination of the funnel plot symmetry. Analysis of 9 studies indicated a significant difference in clinical attachment gain in patients with CAL > 7 mm between the aPDT group and the SRP alone (mean difference=0.92, 95% CI=0.01-1.84, P=0.05) and SRP + AMX/MET (mean difference=0.91, 95% CI=-0.14-1.68, P=0.02) control groups. However, this difference was not significant in patients with CAL < 7 mm. Despite the limitations of the included studies, aPDT can be suggested for the improvement of clinical parameters in grade C molar-incisor pattern Periodontitis with CAL > 7 mm. However, its application in milder cases requires further investigation.

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“…Gram-negativas e fungos(WAINWRIGHT, 2005). No recente estudo in vitro que avaliou o efeito dos corantes AT e AM em diferentes concentrações, associados ao laser vermelho e ao LED, na eliminação de uma suspensão de Aggregatibacter actinomycetemcomitans (Aa), revelou que os corantes em concentração maiores (10mg/ml) são capazes de eliminar o Aa até sem ativação por luz(VALLE et al, 2019).A aPDT também se mostrou efetiva na redução de proteína pró-inflamatórias no FCG. Em uma revisão sistemática de 2016, os pesquisadores encontraram que a aPDT obteve redução significativa de proteínas inflamatórias do FCG na DP para pelo menos uma citocina (como IL-1β, TNF-α, Interferon-γ (IFN-γ), Interleucina-8 (IL-8), Fator estimulante de colônias de Granulócitos (GM-CSF))(AKRAM et al, 2016).…”

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Efeito adjuvante da terapia fotodinâmica antimicrobiana no tratamento periodontal, status salivar, controle glicêmico e qualidade de vida relacionada a saúde oral de pacientes com Diabetes Mellitus tipo 1

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Aos meus pais, Eudes Cunha e Silvia de Oliveira, pelo apoio, incentivo, compreensão e torcida durante todos os anos que estive ausente. Sabendo das histórias de vocês durante a vida escolar até a obtenção do nível Superior, regozijome em dizer que vocês conseguiram mais uma vez. Esse título é nosso.Ao meu irmão, Eudes Junior, pela paciência e assistência em todos meus pedidos (desesperados) por ajuda com notebooks, tabelas, configurações, impressora, arquivo que some, quando ele mesmo tinha dificuldades técnicas por morar no interior do Amazonas. Desculpa, mas os pedidos ainda vão continuar. À minha irmã, Maria Fernanda (MF), que mesmo tão pequena mandava ânimo através de seus áudios e fotos. Agora não precisa mais me convidar para o seu aniversário com 6 meses de antecedência.

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Blue photosensitizers for aPDT eliminate Aggregatibacter actinomycetemcomitans in the absence of light: An in vitro study

Cited by 20 publications

References 44 publications

Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis–related Microorganisms Grown In Vitro

Low-Level Laser and Antimicrobial Photodynamic Therapy Reduce Peri-implantitis–related Microorganisms Grown In Vitro

Clinical attachment loss in the use of adjunctive antimicrobial photodynamic therapy in Stages II-IV Grade C molar-incisor periodontitis: A systematic review and meta-analysis

Efeito adjuvante da terapia fotodinâmica antimicrobiana no tratamento periodontal, status salivar, controle glicêmico e qualidade de vida relacionada a saúde oral de pacientes com Diabetes Mellitus tipo 1

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