BmICE-2 is a novel pro-apoptotic caspase involved in apoptosis in the silkworm, Bombyx mori

Yi, Huashan; Pan, Cai-Xia; Pan, Chun; Song, Juan; Hu, Yanfen; Wang, La; Pan, Min‐Hui; Lü, Cheng

doi:10.1016/j.bbrc.2014.01.139

Cited by 15 publications

(17 citation statements)

References 24 publications

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“…As a promoter of apoptosis, BmICE‐2 participates in BmN‐SWU1 apoptosis, which can be induced by ActD. Therefore, the BmICE‐2 gene might play a key role in the development of B. mori (Yi et al, ). However, the specific mechanism associated with these apoptotic genes remains unclear and requires further investigation.…”

Section: Ampsmentioning

confidence: 99%

“…Based on the available genomic data, 52 apoptosis‐related candidate genes have been identified in silkworms (Courtiade et al, ), including five caspases (Zhang et al, ). Furthermore, BmIce‐2, Bmp53 (Yi et al, ) and BmDronc (Zhang et al, ) can enhance the apoptotic rate of cells. In BmN cells, BmIAP and BmBuffy are involved in inhibiting apoptosis (Pan et al, ) and mitochondrial cytochrome C plays a minor role in regulating Drosophila cell apoptosis (Zhang et al, ).…”

Section: Ampsmentioning

confidence: 99%

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Research progress on the immune mechanism of the silkworm Bombyx mori

et al. 2018

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The silkworm Bombyx mori L., representing an important economic insect and one of the best models for studying insect immunity, possesses an efficient and sophisticated innate immune system against invasive microorganisms. The innate immune system basically includes humoural immunity and cellular immunity. The humoural immunity, which functions via molecules including humoural factors, lysozymes, phenoloxidase, hemolin, lectins and, in particular, antimicrobial peptides, plays a central role in eliminating the invading pathogens. The cellular immunity is primarily carried out and mediated by plasmatocytes and granular cells of haemocytes in the haemolymph, usually followed by melanization. Additionally, apoptosis, a primary viral defence for insects lacking adaptive immunity, comprises an important part of the silkworm immune system. Currently, there is still the lack of a comprehensive and systematic understanding of the molecular mechanisms of silkworm immunity. We review the latest research progress on silkworm immune mechanisms, including phenoloxidase‐dependent melanization and apoptosis, which is conducive to improving our understanding of the silkworm immune mechanism, clarifying the relationship of various immune mechanisms, and also providing a theoretical basis and reference for the future research of insect immunity.

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Section: Ampsmentioning

confidence: 99%

Section: Ampsmentioning

confidence: 99%

Research progress on the immune mechanism of the silkworm Bombyx mori

et al. 2018

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“…Many studies have proven that the Cyt C might play little role in regulating apoptosis in Drosophila . In contrast to that reported in Drosophila , Cyt C release importantly participates in silkworm apoptosis, suggesting that silkworms may be better model systems for apoptosis research [17, 19, 20]. So far, several studies have elucidated that some homologous genes in silkworms also function similarly as in mammals.…”

Section: Introductionmentioning

confidence: 99%

“…So far, several studies have elucidated that some homologous genes in silkworms also function similarly as in mammals. Bm Ice-2, Bm p53 [20] and Bm Dronc [21] have been reported to enhance the rate of apoptosis in Bm N cells, while Bm IAP [22] and Bm Buffy [23] function in inhibition of apoptosis. Bombyx mori death related ced-3/Nedd2-like protein ( Bm Dredd) has relatively high homology with the mammalian initiator caspases which include an N-terminal death effector domain (DED) or caspase recruitment domain (CARD) and a C-terminal CASc domain.…”

Section: Introductionmentioning

confidence: 99%

Role of BmDredd during Apoptosis of Silk Gland in Silkworm, Bombyx mori

et al. 2017

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Silk glands (SGs) undergo massive apoptosis driven degeneration during the larval-pupal transformation. To better understand this event on molecular level, we investigated the expression of apoptosis-related genes across the developmental transition period that spans day 4 in the fifth instar Bombyx mori larvae to day 2 pupae. Increases in the expression of BmDredd (an initiator caspase homolog) closely followed the highest BmEcR expression and resembled the expression trend of BmIcE. Simultaneously, we found that BmDredd expression was significantly higher in SG compared to other tissues at 18 h post-spinning, but reduced following injection of the apoptosis inhibitor (Z-DEVD-fmk). Furthermore, BmDredd expression correlated with changes of caspase3-like activities in SG and RNAi-mediated knockdown of BmDredd delayed SG apoptosis. Moreover, caspase3-like activity was increased in SG by overexpression of BmDredd. Taken together, the results suggest that BmDredd plays a critical role in SG apoptosis.

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“…In mammals, there are two basic signaling pathways that lead to apoptosis. It is unclear whether mitochondria involves in apoptosis in model organism Drosophila (Dorstyn et al., ; Dorstyn and Kumar, ), but some studies found that these fundamental pathways may appear in the silkworm (Yi et al., ). By sequence blast, we have known that Bm Dredd unveils higher homology with mammalian caspase 8 than DmDredd , which has been demonstrated regulating the apoptosis in Drosophila .…”

Section: Discussionmentioning

confidence: 99%

BMDREDD REGULATES THE APOPTOSIS COORDINATING WITH BMDAXX, BMCIDE‐B, BMFADD, AND BMCREB IN BMN CELLS

Chen

Jiao

Lü

et al. 2016

Arch Insect Biochem Physiol

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The apoptosis mechanisms in mammals were investigated relatively clearly. However, little is known about how apoptosis is achieved at a molecular level in silkworm cells. We cloned a caspase homologous gene named BmDredd (where Bm is Bombyx mori and Dredd is death-related ced-3/Nedd2-like caspase) in BmN cells from the ovary of Bm and analyzed its biological information. We constructed the N-terminal, C-terminal, and overexpression vector of BmDredd, respectively. Our results showed that the transcriptional expression level of BmDredd was increased in the apoptotic BmN cells. Furthermore, overexpression of BmDredd increased the caspase-3/7 activity. Simultaneously, RNAi of BmDredd could save BmN cells from apoptosis. The immunofluorescence study showed that BmDredd located at the cytoplasm in normal cell otherwise is found at the nucleus when cells undergo apoptosis. Moreover, we quantified the transcriptional expressions of apoptosis-related genes including BmDredd, BmDaxx (where Daxx is death-domain associated protein), BmCide-b (where Cide-b is cell death inducing DFF45-like effector), BmFadd (Fadd is fas-associated via death domain), and BmCreb (where Creb is cAMP-response element binding protein) in BmN cells with dsRNA interferences to detect the molecular mechanism of apoptosis. In conclusion, BmDredd may function for promoting apoptosis and there are various regulatory interactions among these apoptosis-related genes.

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BmICE-2 is a novel pro-apoptotic caspase involved in apoptosis in the silkworm, Bombyx mori

Cited by 15 publications

References 24 publications

Research progress on the immune mechanism of the silkworm Bombyx mori

Research progress on the immune mechanism of the silkworm Bombyx mori

Role of BmDredd during Apoptosis of Silk Gland in Silkworm, Bombyx mori

BMDREDD REGULATES THE APOPTOSIS COORDINATING WITH BMDAXX, BMCIDE‐B, BMFADD, AND BMCREB IN BMN CELLS

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