BMP-2 modulates expression of other growth factors in a rat fracture healing model

Cottrell, Jessica; Keane, Owen; O’Connor, J. P.

doi:10.1016/j.jab.2014.02.003

Cited by 11 publications

(9 citation statements)

References 35 publications

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“…Previous study have stimulated endogenous BMP-2 expression using exogenous rhBMP-2 [29] and found that BMP-2 expression changes during the healing process. For example, treatment with rhBMP-2 stimulated BMP-2 expression on day 4 but reduced it on days 2 and 10 in a rat fracture model [29]. Further investigations are needed to evaluate endogenous BMP-2 expression at multiple time points.…”

Section: Discussionmentioning

confidence: 99%

“…Finally, we did not evaluate endogenous BMP-2 expression, which plays an important role in bone healing [27,28]. Previous study have stimulated endogenous BMP-2 expression using exogenous rhBMP-2 [29] and found that BMP-2 expression changes during the healing process. For example, treatment with rhBMP-2 stimulated BMP-2 expression on day 4 but reduced it on days 2 and 10 in a rat fracture model [29].…”

Section: Discussionmentioning

confidence: 99%

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Poly(POG)n loaded with recombinant human bone morphogenetic protein-2 accelerates new bone formation in a critical-sized bone defect mouse model

et al. 2020

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Background Delivery of bone morphogenetic protein-2 (BMP-2) via animal-derived absorbable collagen materials is used for the treatment of large bone defects. However, the administration of bovine proteins to humans is associated with the risk of zoonotic complications. We therefore examined the effect of combining BMP-2 with collagen-like peptides, poly(POG)n, in a critical-sized bone defect mouse model. Methods A 2-mm critical-sized bone defect was created in the femur of 9-week-old male C57/BL6J mice. Mice were randomly allocated into one of four treatment groups (n = 6 each): control (no treatment), poly(POG)n only, 0.2 μg, or 2.0 μg BMP-2 with poly(POG)n. New bone formation was monitored using soft X-ray radiographs, and bone formation at the bone defect site was examined using micro-computed tomography and histological examination at 4 weeks after surgery. Results Administration of 2.0 μg of BMP-2 with poly(POG)n promoted new bone formation and resulted in greater bone volume and bone mineral content than that observed in the control group and successfully achieved consolidation. In contrast, bone formation in all other groups was scarce. Conclusions Our findings suggest the potential of BMP-2 with poly(POG)n as a material, free from animal-derived collagen, for the treatment of large bone defects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Poly(POG)n loaded with recombinant human bone morphogenetic protein-2 accelerates new bone formation in a critical-sized bone defect mouse model

et al. 2020

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“…They also showed that only the expression of genes encoding for BMP-2 and BMP-4 changes over time. The highest mRNA level of BMP-2, BMP-4 (as well as TNF-α), at 21 days, is in accordance with an increase in osteoclast resorption activity during callus bone remodeling [ 336 ]. The expression of Ser/Thr kinase receptors can also vary during bone fracture healing [ 342 ].…”

Section: Effect Of Tgf-β Superfamily On Bone Homeostasis and Diseamentioning

confidence: 95%

“…The members of the TGF-β superfamily were detected in all phases of the bone fracture healing, but their temporal expression patterns and amounts might vary in function of the animal model used [ 334 , 335 , 336 ]. Their effects are also coordinated with those of inflammatory cytokines, other growth factors (PDGF, VEGF, FGF1, and FGF2) and extracellular matrix proteins like type I collagen [ 337 , 338 , 339 ].…”

Section: Effect Of Tgf-β Superfamily On Bone Homeostasis and Diseamentioning

confidence: 99%

Influence of the TGF-β Superfamily on Osteoclasts/Osteoblasts Balance in Physiological and Pathological Bone Conditions

Jann

Gascon

Roux

et al. 2020

IJMS

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The balance between bone forming cells (osteoblasts/osteocytes) and bone resorbing cells (osteoclasts) plays a crucial role in tissue homeostasis and bone repair. Several hormones, cytokines, and growth factors—in particular the members of the TGF-β superfamily such as the bone morphogenetic proteins—not only regulate the proliferation, differentiation, and functioning of these cells, but also coordinate the communication between them to ensure an appropriate response. Therefore, this review focuses on TGF-β superfamily and its influence on bone formation and repair, through the regulation of osteoclastogenesis, osteogenic differentiation of stem cells, and osteoblasts/osteoclasts balance. After introducing the main types of bone cells, their differentiation and cooperation during bone remodeling and fracture healing processes are discussed. Then, the TGF-β superfamily, its signaling via canonical and non-canonical pathways, as well as its regulation by Wnt/Notch or microRNAs are described and discussed. Its important role in bone homeostasis, repair, or disease is also highlighted. Finally, the clinical therapeutic uses of members of the TGF-β superfamily and their associated complications are debated.

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“…BMPs are the most effective boneinducing growth factors, belonging to the TGF-β superfamily, which are multifunctional cytokines. BMPs can signi cantly induce the different stages of the bone healing process such as the in ammatory phase, angiogenesis, the callus formation, and bone remodeling 7 . VEGF improves angiogenesis via induce effects in endothelial cells 8 .…”

Section: Introductionmentioning

confidence: 99%

The healing capacity and osteogenesis pattern of Demineralized Dentin Matrix (DDM)-Fibrin Glue (FG) compound

Bao

et al. 2023

Preprint

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Demineralized dentin matrix (DDM) is an osteoconductive and osteoinductive material, which was successfully performed in sinus floor augmentation and alveolar ridge augmentation in clinical application. It releases bone morphogenetic protein (BMP) and other growth factors which make DDM a suitable grafting material. However, the granular particle of DDM makes it difficult to anchor into the bone defect area. The aim of this study was to investigate biological effects as well as an osteoinductivity of the combination of DDM and Fibrin Glue (FG) at an optimal ratio on bone healing from critical bone defect in an animal model. Mouse osteoblastic cell line (MC3T3-E1) was co-cultured with various ratios of DDM and FG to examine their effects on osteoblast proliferation and differentiation indicated by alkaline phosphatase (ALP) activity, osteocalcin (OC) production and mineralized nodules formation. The optimal ratio was then chosen to further study with rabbit calvarial defective model which they were implanted with DDM or DDM-FG1 (1:0.1) and DDM-FG2 (1:0.5) compounds (w/v) or blank for 2, 4, 8 and 12weeks for investigating soft tissue and new bone regeneration. Micro-CT and histology analysis were used to evaluate the total grafting properties according to the different healing periods. The result from in vitro studies demonstrated that the ratio of 1:0.1 induced more ALP activity, mineralized nodules at the same time, 1: 0.5 (DDM-FG combined) induced more Osteocalcin (OC) at given days. In animal model, the 3D new bone volume in all DDM-FG treatment were significantly greater than blank group at 2, 4, 8 and 12 weeks. Furthermore, the new bone volume was greater in DDM-FG2 when compared to other groups at early week of the healing period. In histological analysis, numbers of osteoblasts were formed as a cluster adjacent to the DDM particles where we could observe a newly formed bone in all groups suggesting an osteoinductive property of DDM. Moreover, the greater new collagen synthesis observed at 4 weeks suggested that the early bone healing was induced in a group treated with DDM-FG2. This study demonstrates that at optimal ratio of DDM-FG compound enhances the osteogenic activities and bone regeneration.

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BMP-2 modulates expression of other growth factors in a rat fracture healing model

Cited by 11 publications

References 35 publications

Poly(POG)n loaded with recombinant human bone morphogenetic protein-2 accelerates new bone formation in a critical-sized bone defect mouse model

Poly(POG)n loaded with recombinant human bone morphogenetic protein-2 accelerates new bone formation in a critical-sized bone defect mouse model

Influence of the TGF-β Superfamily on Osteoclasts/Osteoblasts Balance in Physiological and Pathological Bone Conditions

The healing capacity and osteogenesis pattern of Demineralized Dentin Matrix (DDM)-Fibrin Glue (FG) compound

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