BMP-2-releasing gelatin microspheres/PLGA scaffolds for bone repairment of X-ray-radiated rabbit radius defects

Xia, Peng; Wang, Song; Qi, Zhiping; Zhang, Wei; Sun, Yun-Yu

doi:10.1080/21691401.2019.1594852

Cited by 34 publications

(29 citation statements)

References 52 publications

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“…Furthermore, locally high doses of rhBMP-2 can cause significant adverse reactions [57,58]. Gelatin microspheres have been demonstrated to be an ideal sustainedrelease carrier for rhBMP-2 in previous studies [38,59]. In addition, the low immunogenicity, good biocompatibility, and biodegradability of gelatin have been demonstrated [60].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an appropriate carrier for rhBMP-2 is needed to achieve stable and sustained release. Xia et al [38] used gelatin microspheres to encapsulate BMP-2 on a poly(lactic-co-glycolic acid) scaffold and achieved sustained release. In addition, the coating also enhanced the proliferation and osteogenesis of rabbit bone mesenchymal stem cells (BMSCs) in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced osteoinductivity and corrosion resistance of dopamine/gelatin/rhBMP-2–coated β-TCP/Mg-Zn orthopedic implants: An in vitro and in vivo study

et al. 2020

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Magnesium-based biomaterials are attracting increasingly more attention for orthopedic applications based on their appropriate mechanical properties, biodegradability, and favorable biocompatibility. However, the high corrosion rate of these materials remains to be addressed. In this study, porous β-Ca 3 (PO 4) 2 /Mg-Zn (β-TCP/Mg-Zn) composites were fabricated via a powder metallurgy method. The β-TCP/Mg-Zn composites with 6% porosity exhibited optimal mechanical properties, and thus, they were selected for surface modification. A novel dopamine/gelatin/recombinant human bone morphogenetic protein-2 (rhBMP-2) coating with demonstrated stability was prepared to further improve the corrosion resistance of the composite and enhance early osteoinductivity. The homogeneously coated β-TCP/Mg-Zn composite showed significantly improved corrosion resistance according to electrochemical and immersion tests. In addition, extracts from the dopamine/ gelatin/rhBMP-2-coated β-TCP/Mg-Zn composite not only facilitated cell proliferation but also significantly enhanced the osteogenic differentiation of Sprague-Dawley rat bone marrow-derived mesenchymal stem cells in vitro. Furthermore, in vivo experiments were performed to evaluate the biodegradation, histocompatibility, and osteoinductive potential of the coated composite. No obvious pathological changes in the vital visceral organs were observed after implantation, and radiography and hematoxylin-eosin staining showed strong promotion of new bone formation, matched composite degradation and bone regeneration rates, and complete absorption of the released hydrogen gas. Collectively, these results indicate that the dopamine/gelatin/rhBMP-2-coated β-TCP/Mg-Zn composite offers improved corrosion resistance, favorable biocompatibility, and enhanced osteoinductive potential for use in the fabrication of orthopedic implants.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced osteoinductivity and corrosion resistance of dopamine/gelatin/rhBMP-2–coated β-TCP/Mg-Zn orthopedic implants: An in vitro and in vivo study

et al. 2020

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“…They found that BMP-2 scaffolds increased osteogenesis and proliferation of BMSCs in vitro. In vivo, BMP-2 scaffolds showed significantly higher osteogenic potential and sustained release of the GF [ 58 ]. Another GF commonly used in tissue engineering is VEGF.…”

Section: Desired Characteristics Of 3d Scaffoldsmentioning

confidence: 99%

Tissue Engineering Strategies for Treating Avascular Necrosis of the Femoral Head

et al. 2021

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Avascular necrosis (AVN) of the femoral head commonly leads to symptomatic osteoarthritis of the hip. In older patients, hip replacement is a viable option that restores the hip biomechanics and improves pain but in pediatric, adolescent, and young adult patients hip replacements impose significant activity limitations and the need for multiple revision surgeries with increasing risk of complication. Early detection of AVN requires a high level of suspicion as diagnostic techniques such as X-rays are not sensitive in the early stages of the disease. There are multiple etiologies that can lead to this disease. In the pediatric and adolescent population, trauma is a commonly recognized cause of AVN. The understanding of the pathophysiology of the disease is limited, adding to the challenge of devising a clinically effective treatment strategy. Surgical techniques to prevent progression of the disease and avoid total hip replacement include core decompression, vascular grafts, and use of bone-marrow derived stem cells with or without adjuncts, such as bisphosphonates and bone morphogenetic protein (BMP), all of which are partially effective only in the very early stages of the disease. Further, these strategies often only improve pain and range of motion in the short-term in some patients and do not predictably prevent progression of the disease. Tissue engineering strategies with the combined use of biomaterials, stem cells and growth factors offer a potential strategy to avoid metallic implants and surgery. Structural, bioactive biomaterial platforms could help in stabilizing the femoral head while inducing osteogenic differentiation to regenerate bone and provide angiogenic cues to concomitantly recover vasculature in the femoral head. Moreover, injectable systems that can be delivered using a minimal invasive procedure and provide mechanical support the collapsing femoral head could potentially alleviate the need for surgical interventions in the future. The present review describes the limitations of existing surgical methods and the recent advances in tissue engineering that are leading in the direction of a clinically effective, translational solution for AVN in future.

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“…Recently, growth factors have been carried in GMs to be delivered. Compared with other forms of carriers, the advantages of microspheres are that they can extend the h55alf-life of growth factors that they carry [34], and consequently improve the therapeutic effect in vivo [22,35]. GMs loading PRP has been applied for tissue regeneration, such as treatment in the osteoarthritis in rabbit [36], treatment for the degenerated intervertebral disc (IVD) model in rabbit [37,38], bone regeneration in rabbit [39,40], and treatment of ischaemic disorders in mouse [41].…”

Section: Ivyspringmentioning

confidence: 99%

Injectable gelatin microspheres loaded with platelet rich plasma improve wound healing by regulating early inflammation

Zhou¹,

Li²,

Chen³

et al. 2021

Int. J. Med. Sci.

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We investigated the potential of gelatin microspheres (GMs) loaded with platelet-rich plasma (PRP) to enhance their wound healing effect. Platelets from the PRP were immobilized onto GMs to form biomimetic bioreactor GM+PRP. The therapeutic effect of this agent was further investigated in vivo on a wound-healing model in rats. Wounds were locally injected with phosphate buffered saline (PBS), GM, PRP, and GM+PRP. Wound healing rate, vessel density, and inflammation level were measured histologically, by RT-PCR, and by Western blotting at days 3, 7, 14, and 21. Platelets on GM caused a continuous high release in both interleukin-10 and metalloproteinase-3 compared with PRP alone. Both GM+PRP and PRP successfully accelerated the wound healing process, while GM alone did not improve the wound healing process compared with the untreated control. Wounds treated with GM+PRP resulted in shorter healing period and improved dermal structure. GM+PRP improved angiogenesis in the wound by increasing expression of angiogenic factors. GM+PRP prolonged and enhanced the cytokine release profile compared with PRP. By promoting the inflammatory and angiogenic responses, GM+PRP has the potential to improve wound healing. Our findings demonstrate that GMs are an injectable carrier that enhanced the therapeutic effects of PRP.

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BMP-2-releasing gelatin microspheres/PLGA scaffolds for bone repairment of X-ray-radiated rabbit radius defects

Abstract: View related articles View Crossmark data Citing articles: 4 View citing articles BMP-2-releasing gelatin microspheres/PLGA scaffolds for bone repairment of X-ray-radiated rabbit radius defects

Cited by 34 publications

References 52 publications

Enhanced osteoinductivity and corrosion resistance of dopamine/gelatin/rhBMP-2–coated β-TCP/Mg-Zn orthopedic implants: An in vitro and in vivo study

Enhanced osteoinductivity and corrosion resistance of dopamine/gelatin/rhBMP-2–coated β-TCP/Mg-Zn orthopedic implants: An in vitro and in vivo study

Tissue Engineering Strategies for Treating Avascular Necrosis of the Femoral Head

Injectable gelatin microspheres loaded with platelet rich plasma improve wound healing by regulating early inflammation

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