BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development

Díaz, Pablo Uriel; Hein, Gustavo Juan; Belotti, Eduardo Matías; Rodríguez, Fernanda Mariel; Rey, Florencia; Amweg, Ayelén Noelia; Matiller, Valentina; Baravalle, María Eugenia; Ortega, Hugo Héctor; Salvetti, Natalia Raquel

doi:10.1530/rep-15-0315

Cited by 20 publications

(8 citation statements)

References 63 publications

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“…During folliculogenesis, the abundance of BMP2 increases in GCs of early antral follicles. Given the results of our research are according to the findings of Diaz et al [54], the expression of BMP2 in GCs was higher in antral follicles [54]. The expression pattern is concordant with the previously illustrated expression pattern of BMP2 in the ovarian somatic cells [24].…”

Section: Discussionsupporting

confidence: 92%

Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones

Umer

Sammad

Zou

et al. 2019

Genes

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Anti-Mullerian hormone (AMH) is an important reproductive marker of ovarian reserve produced by granulosa cells (GCs) of pre-antral and early-antral ovarian follicles in several species, including cattle. This hormone plays a vital role during the recruitment of primordial follicles and follicle stimulating hormone (FSH)-dependent follicular growth. However, the regulatory mechanism of AMH expression in follicles is still unclear. In this study, we compared the expression of AMH, AMHR-II, BMP2, BMP6, FSHR, and LHCGR genes during follicular development. In-vitro expression study was performed with and without FSH for AMH, AMHR-II, BMP2, and BMP6 genes in bovine GCs which were isolated from 3-8 mm follicles. Association among the mRNA expression and hormone level was estimated. GCs were collected from small (3-8 mm), medium (9-12 mm) and large size (13 to 24 mm) follicles before, during onset, and after deviation, respectively. Further, mRNA expression, hormones (AMH, FSH, and LH), apoptosis of GCs, and cell viability were detected by qRT-PCR, ELISA, flow cytometry, and spectrophotometry. AMH, AMHR-II, BMP2, and FSHR genes were highly expressed in small and medium follicles as compared to large ones. In addition, the highest level of AMH protein (84.14 ± 5.41 ng/mL) was found in medium-size follicles. Lower doses of FSH increased the viability of bovine GCs while higher doses repressed them. In-vitro cultured GCs treated with FSH significantly increased the AMH, AMHR-II, and BMP2 expression levels at lower doses, while expression levels decreased at higher doses. We found an optimum level of FSH (25 ng/mL) which can significantly enhance AMH and BMP2 abundance (p < 0.05). In summary, AMH, AMHR-II, and BMP2 genes showed a higher expression in follicles developed in the presence of FSH. However, lower doses of FSH demonstrated a stimulatory effect on AMH and BMP2 expression, while expression started to decline at the maximum dose. In this study, we have provided a better understanding of the mechanisms regulating AMH, AMHR II, and BMP2 signaling in GCs during folliculogenesis, which would improve the outcomes of conventional assisted reproductive technologies (ARTs), such as superovulation and oestrus synchronization in bovines.

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Section: Discussionsupporting

confidence: 92%

Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones

Umer

Sammad

Zou

et al. 2019

Genes

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“…Although ACVR2 binds activins, it has been found that ACVR2B also binds inhibin A/B, and BMP2, 6 and 7 (Caestecker, ). Results found by Díaz et al () indicated an alteration in the expression of BMPs in cows with COD, especially a higher expression of BMP4 and BMP6 as well as of BMPR1B, which occurs early in folliculogenesis and incipiently during the development of the disease. Consequently, we can hypothesize that high expression of these ligands and their receptors in ovarian structures of cows with COD exacerbates the function of these ligands.…”

Section: Discussionmentioning

confidence: 93%

“…Although ACVR2 binds activins, it has been found that ACVR2B also binds inhibin A/B, and BMP2, 6 and 7 (Caestecker, 2004). Results found by Díaz et al (2016) indicated an alteration in the expression of BMPs in cows with COD, especially a higher expression of BMP4 and BMP6…”

Section: Discussionmentioning

confidence: 99%

“…Ovarian ultrasonographic examinations were performed daily in all animals until ovariectomy in both groups (Figure ), using a real‐time, B‐mode scanner equipped with a 5 MHz, linear‐array, transrectal transducer (Honda HS101V, Japan) (Díaz et al, ). Follicular cysts detected by ultrasonography were defined as any follicular structure with a diameter equal to or greater than 20 mm present for 10 days or more, without ovulation or CL formation (Silvia et al, ).…”

Section: Methodsmentioning

confidence: 99%

“…Follicular dynamics were followed by daily ultrasound scanning, and blood steroid hormones were measured as indicated. Ovariectomy was performed to obtain preovulatory follicles from control animals and cysts persisting for at least 10 d from treated animals transrectal transducer (Honda HS101V, Japan) (Díaz et al, 2016). Follicular cysts detected by ultrasonography were defined as any follicular structure with a diameter equal to or greater than 20 mm present for 10 days or more, without ovulation or CL formation (Silvia et al, 2002).…”

Section: Induction Of Cod By Adrenocorticotropin (Acth) Administrationmentioning

confidence: 99%

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Expression of TGFBR1, TGFBR2, TGFBR3, ACVR1B and ACVR2B is altered in ovaries of cows with cystic ovarian disease

Matiller

Hein

Stassi

et al. 2019

Reprod Domestic Animals

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The objective of the present study was to examine the expression of transforming growth factor beta receptor (TGFBR)1, TGFBR2, TGFBR3, Activin Receptor (ACVR)1B and ACVR2B in ovaries of cows with Cystic Ovarian Disease (COD). The expression of the selected receptors was determined by immunohistochemistry in sections of ovaries from cows with ACTH-induced and spontaneous COD. Expression of TGFBR1 and TGFBR3 was higher in granulosa cells of cysts from cows with spontaneous COD than in tertiary follicles from the control group. Additionally, TGFBR3 expression was higher in granulosa cells of cysts from cows with ACTH-induced COD than in those from the control group and lower in theca cells of spontaneous and ACTH-induced cysts than in tertiary control follicles. There were no changes in the expression of TGFBR2. ACVR1B expression was higher in granulosa cells of tertiary follicles of cows with spontaneous COD than in the control group, whereas ACVR2B expression was higher in cysts of the spontaneous COD group than in tertiary follicles from the control group. The alterations here detected, together with the altered expression of the ligands previously reported, indicate alterations in the response of the ligands in the target cells, modifying their actions at cellular level. This article is protected by copyright. All rights reserved.

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The novel C268A variant of BMP2 is linked to the reproductive performance of Awassi and Hamdani sheep

Imran,

Al-Thuwaini

2024

Mol Biol Rep

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BMP2, 4 and 6 and BMPR1B are altered from early stages of bovine cystic ovarian disease development

Cited by 20 publications

References 63 publications

Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones

Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones

Expression of TGFBR1, TGFBR2, TGFBR3, ACVR1B and ACVR2B is altered in ovaries of cows with cystic ovarian disease

The novel C268A variant of BMP2 is linked to the reproductive performance of Awassi and Hamdani sheep

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