BMP3 promoter hypermethylation in plasma-derived cell-free DNA in colorectal cancer patients

Rokni, Parisa; Shariatpanahi, Afsaneh Mojtabanezhad; Sakhinia, Ebrahim; Kerachian, Mohammad Amin

doi:10.1007/s13258-017-0644-2

Cited by 36 publications

(22 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During recent years, the non-protein biomarkers so-called "liquid biopsy" of circulating tumor cells and circulating DNA or exosome products has drawn great attention 4,15 . Developing new biomarkers is relied on understanding the mechanisms exploited by cancer.…”

mentioning

confidence: 99%

Crosstalk between DNA methylation and gene expression in colorectal cancer, a potential plasma biomarker for tracing this tumor

Kerachian

Javadmanesh

Azghandi

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Colorectal cancer (CRC), the second leading cause of cancer mortality, constitutes a significant global health burden. An accurate, noninvasive detection method for cRc as complement to colonoscopy could improve the effectiveness of treatment. In the present study, SureSelectXT Methyl-Seq was performed on cancerous and normal colon tissues and CLDN1, INHBA and SLC30A10 were found as candidate methylated genes. MethyLight assay was run on formalin-fixed paraffin-embedded (FFPE) and fresh case and control tissues to validate the methylation of the selected gene. the methylation was significantly different (p-values < 2.2e-16) with a sensitivity of 87.17%; at a specificity cut-off of 100% in FFPE tissues. Methylation studies on fresh tissues, indicated a sensitivity of 82.14% and a specificity cut-off of 92% (p-values = 1.163e-07). The biomarker performance was robust since, normal tissues indicated a significant 22.1-fold over-expression of the selected gene as compared to the corresponding CRC tissues (p-value < 2.2e-16) in the FFPE expression assay. In our plasma pilot study, evaluation of the tissue methylation marker in the circulating cell-free DNA, demonstrated that 9 out of 22 CRC samples and 20 out of 20 normal samples were identified correctly. In summary, there is a clinical feasibility that the offered methylated gene could serve as a candidate biomarker for CRC diagnostic purpose, although further exploration of our candidate gene is warranted.Colorectal cancer (CRC) constitutes a significant global health burden, leading to over 862,000 deaths globally in 2018. It is the second main cause of cancer mortality in the world and currently stands as the third most common cancer, with a yearly incidence of over one million and eight thousand cases worldwide 1 . Its leading cause of death is due to liver metastasis with a median survival rate of approximately 30 months. Generally, half of the patients with CRC develop tumor recurrences 2 . For early-diagnosed CRC patients, the 5-year survival rates are approximately 90% but this lowers to less than 10% in patients with extensive metastases. Thus, the most effective approach to reduce CRC incidence and its mortality is early detection of colonic lesions 3 . Fortunately, because of implementation and growth of wide spread cancer screening assays, such as colonoscopy as well as increasingly effective therapies, the mortality rate of CRC is lowering in many countries 2 .

show abstract

mentioning

confidence: 99%

Crosstalk between DNA methylation and gene expression in colorectal cancer, a potential plasma biomarker for tracing this tumor

Kerachian

Javadmanesh

Azghandi

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sample handling and the techniques used for cfDNA analysis are one of the major hindrances in cfDNA studies [ 9 , 10 ]. Pre-analytical factors i.e.…”

Section: Main Textmentioning

confidence: 99%

Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation

et al. 2018

Self Cite

View full text Add to dashboard Cite

ObjectiveIn the present study, we investigated different simple and cost effective methods to evaluate and validate cell free DNA (cfDNA) isolation. The ability of the QIAamp DNA Blood Mini Kit method to extract cfDNA was assessed by several approaches, including purification of endogenous cfDNA and exogenous spike-in control material, prior to plasma extraction, and followed by quantitative-PCR.ResultsUsing QIAamp DNA Blood Mini kit, nearly 27% (380 bp) to 35% (173 bp) cfDNA was recovered with a higher recovery of smaller size cfDNA (173 bp) in comparison to larger ones (380 bp). These simple laboratory methods can be used to assess the efficiency of any cfDNA isolation method.Electronic supplementary materialThe online version of this article (10.1186/s13104-018-3866-8) contains supplementary material, which is available to authorized users.

show abstract

“…The incidence of colorectal cancer (CRC) has rapidly increased globally [1]. It is the second most common cancer and the fourth leading cause of cancer death (about 1,400,000 new cases and about 700,000 deaths) in the world.…”

Section: Introductionmentioning

confidence: 99%

Association of MTHFR C677T Variant Genotype with Serum Folate and Vit B12 in Iranian Patients with Colorectal Cancer or Adenomatous Polyps

Ghorbani

Baharara

Kerachian

2020

Preprint

Self Cite

View full text Add to dashboard Cite

Background: The incidence of colorectal cancer (CRC) has increased vigorously during the last decades in Iran and clinical studies suggest that moderate deficiency of methylenetetrahydrofolate reductase (MTHFR) and essential folate dietary intake may reduce the risk of CRC.The aim of this study was to investigate the clinical significance of C677T polymorphism within MTHFR gene and its correlation with the serum folate and Vit B12 in Iranian population suffering from CRC.Methods: Blood samples were taken from 207 Iranian individuals (103 males and 104 females) who were referred for colonoscopy. TaqMan probe assay was performed for C677T MTHFR polymorphism. Besides, sera were fractionated from the blood samples of 43 patients and controls and folate and Vit B12 concentrations were measured by a monobind kit. Finally, the correlation of MTHFR polymorphisms and folate/vitamin-B12 with CRC risk were analyzed.Results: In the current study, the most percentage of the MTHFR polymorphisms in healthy individuals belongs to CC genotype (wild type). In patients with adenoma the percentage of CC and CT genotype were approximately similar, but surprisingly in adenocarcinoma the percentage of CC genotype (wild type) was the highest (approximately 50%), and the percentage of CT and TT genotypes were slightly similar. Our study revealed that there was no significant difference between the amount of folate and Vit B12 in case and control groups (p value >0.05).Conclusions: We concluded that there is an association between pivotal concentration of serum folate/vitamin B12 and CRC, and this association is altered by MTHFR C677T genotype.

show abstract

BMP3 promoter hypermethylation in plasma-derived cell-free DNA in colorectal cancer patients

Cited by 36 publications

References 33 publications

Crosstalk between DNA methylation and gene expression in colorectal cancer, a potential plasma biomarker for tracing this tumor

Crosstalk between DNA methylation and gene expression in colorectal cancer, a potential plasma biomarker for tracing this tumor

Simple and cost-effective laboratory methods to evaluate and validate cell-free DNA isolation

Association of MTHFR C677T Variant Genotype with Serum Folate and Vit B12 in Iranian Patients with Colorectal Cancer or Adenomatous Polyps

Contact Info

Product

Resources

About