BMSCs overexpressed ISL1 reduces the apoptosis of islet cells through ANLN carrying exosome, INHBA, and caffeine

Wang, Ying; Wang, Jingwen; Wang, Jiale; Zhang, Shu-Cong; Ma, Ruiyang; Zhang, Jing; Li, Yang; Liu, Peijun; Xue, Wujun; Zheng, Jin; Ding, Xiaoming

doi:10.1007/s00018-022-04571-0

Cited by 7 publications

(9 citation statements)

References 65 publications

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“…Wang et al 48 demonstrated that MSCs promoted the expression of islet insulin gene enhancer binding protein 1 (ISL1) and led to an increase in VEGF in islet grafts. Subsequently, MSCs transfected with high ISL1 expression have been utilized to further demonstrate that ISL1 promotes the release of exosomes of aniline from BMSCs, secretion of Inhibin beta A chain and caffeine, and reduction of streptozotocin (STZ)-induced islet apoptosis 49 . In addition, it has also been shown in many studies that islet and MSC co-transplantation shortens the post-transplantation ischemic period by increasing neovascularization 50 , 51 .…”

Section: Merits Of Co-culture and Co-transplantation Of Mscs And Isletsmentioning

confidence: 99%

“…Although renal peritoneal transplantation site is less difficult to perform the procedure on and less likely to result in rejection, the smaller capacity makes it difficult to meet the needs of larger patients, and it is more susceptible to islet damage and necrosis. Wang et al 49 transfected BMSCs with ISL1 adenovirus to obtain BMSC-ISL1; then, the authors injected these cells with islet cells in the renal capsule of rats with diabetes. Co-transplantation of the islet cells with BMSCs-ISL1 increased graft survival and prevented graft apoptosis and thus achieved euglycemia by day 5.…”

Section: Sites For Islets/mscs Co-transplantationmentioning

confidence: 99%

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Strategy for Clinical Setting of Co-transplantation of Mesenchymal Stem Cells and Pancreatic Islets

Mei,

Yuwei,

Weiping

et al. 2024

Cell Transplant

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Islet transplantation may be the most efficient therapeutic technique for patients with type 1 diabetes mellitus (T1DM). However, the clinical application of this method is faced with numerous limitations, including isolated islet apoptosis, recipient rejection, and graft vascular reconstruction. Mesenchymal stem cells (MSCs) possess anti-apoptotic, immunomodulatory, and angiogenic properties. Here, we review recent studies on co-culture and co-transplantation of islets with MSCs. We have summarized the methods of preparation of co-transplantation, especially the merits of co-culture, and the effects of co-transplantation. Accumulating experimental evidence shows that co-culture of islets with MSCs promotes islet survival, enhances islet secretory function, and prevascularizes islets through various pretransplant preparations. This review is expected to provide a reference for exploring the use of MSCs for clinical islet co-transplantation.

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Section: Merits Of Co-culture and Co-transplantation Of Mscs And Isletsmentioning

confidence: 99%

Section: Sites For Islets/mscs Co-transplantationmentioning

confidence: 99%

Strategy for Clinical Setting of Co-transplantation of Mesenchymal Stem Cells and Pancreatic Islets

Mei,

Yuwei,

Weiping

et al. 2024

Cell Transplant

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“…1). Many studies have found that MSCT significantly reduce the apoptosis of diseases-related tissue cells (Table 1), which is achieved by the activation of a variety of signaling pathways [13][14][15]. More interestingly, MSCs also alleviate diseases by promoting apoptosis in some cells, such as autoimmune diseases and tumors [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

The dual role of mesenchymal stem cells in apoptosis regulation

Chen,

Xia,

Yao

et al. 2024

Cell Death Dis

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Mesenchymal stem cells (MSCs) are widely distributed pluripotent stem cells with powerful immunomodulatory capacity. MSCs transplantation therapy (MSCT) is widely used in the fields of tissue regeneration and repair, and treatment of inflammatory diseases. Apoptosis is an important way for tissues to maintain cell renewal, but it also plays an important role in various diseases. And many studies have shown that MSCs improves the diseases by regulating cell apoptosis. The regulation of MSCs on apoptosis is double-sided. On the one hand, MSCs significantly inhibit the apoptosis of diseased cells. On the other hand, MSCs also promote the apoptosis of tumor cells and excessive immune cells. Furthermore, MSCs regulate apoptosis through multiple molecules and pathways, including three classical apoptotic signaling pathways and other pathways. In this review, we summarize the current evidence on the regulation of apoptosis by MSCs.

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“…Insulin gene enhancer binding protein 1 (ISL1), a LIM homeodomain transcription factor, has been reported to play vital roles in cardiogenesis, neuronal development, cancer progression, and the maturation of islet cells [26][27][28]. Our previous work demonstrated that ISL1-overexpressing bone marrow mesenchymal stem cells (BMSCs) reduce the apoptosis of grafted islets through the paracrine function [29]. It has been reported that ISL1 overexpression in human MSCs promoted cell survival and improved cardiac function in the model of myocardial infarction [30].…”

Section: Introductionmentioning

confidence: 99%

ISL1-overexpressing BMSCs attenuate renal ischemia-reperfusion injury by suppressing apoptosis and oxidative stress through the paracrine action

Wang,

et al. 2024

Preprint

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Ischemia-reperfusion injury (IRI) is a major event in renal transplantation, leading to adverse outcomes. Bone marrow mesenchymal stem cells (BMSCs) are novel promising therapeutics for repairing kidney injuries. The therapeutic efficacy of BMSCs with ISL1 overexpression in renal IRI and its underlying mechanism need to be investigated. The unilateral renal IRI rat model was established to mimic clinical acute kidney injury. Rats were injected with PBS, BMSCs-Scrambled or BMSCs-ISL1 via the tail vein at the timepoint of reperfusion, and then sacrificed after 24 h of reperfusion. The administration of BMSCs-ISL1 significantly improved renal function, inhibited tubular cells apoptosis, inflammation, oxidative stress in rats. In vitro, HKC cells subjected to H2O2 stimulation were pretreated with the conditioned medium (CM) of BMSCs-Scrambled or BMSCs-ISL1. The pretreatment of ISL1-CM attenuated apoptosis and oxidative stress induced by H2O2 in HKC cells. Our proteomic data suggested that haptoglobin (Hp) was one of the secretory proteins in ISL1-CM. Subsequent experiments confirmed that Hp was the important paracrine factor from BMSCs-ISL1 that exerted anti-apoptotic and antioxidant functions. Mechanistically, Hp played a cytoprotective role via the inhibition of ERK signaling pathway, which could be abrogated by Ro 67-7476, the ERK phosphorylation agonist. The results suggested that paracrine action may be the main mechanism for BMSCs-ISL1 to exert protective effects. As an important anti-apoptotic and antioxidant factor in ISL1-CM, Hp may serve as a new therapeutic agent for treating IRI, providing new insights for overcoming the long-term adverse effects of stem cell therapy.

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BMSCs overexpressed ISL1 reduces the apoptosis of islet cells through ANLN carrying exosome, INHBA, and caffeine

Cited by 7 publications

References 65 publications

Strategy for Clinical Setting of Co-transplantation of Mesenchymal Stem Cells and Pancreatic Islets

Strategy for Clinical Setting of Co-transplantation of Mesenchymal Stem Cells and Pancreatic Islets

The dual role of mesenchymal stem cells in apoptosis regulation

ISL1-overexpressing BMSCs attenuate renal ischemia-reperfusion injury by suppressing apoptosis and oxidative stress through the paracrine action

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