Boar semen proteomics and sperm preservation

Parrilla, Inmaculada; Pérez-Patiño, Cristina; Li, J.; Barranco, Isabel; Padilla, Lorena; Rodriguez‐Martinez, Heriberto; Martı́nez, Emilio A.; Roca, Jordi

doi:10.1016/j.theriogenology.2019.05.033

Cited by 40 publications

(35 citation statements)

References 46 publications

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“…It is to note that apart from AWN, there is no overlap with proteins associated with boar fertility or semen preservability identified in previous high-throughput proteomics [17,31]. As stated above, varying results could be explained by different workflows of protein separation and identification procedures [17]. Therefore, confirmation of proteomic results and further experiments (e.g., the depletion of these proteins) are needed to elucidate the functional role for maintenance of sperm viability during on vitro storage and ultimately fertility.…”

Section: Discussionmentioning

confidence: 87%

“…However, a specific action of this protein on sperm function is unknown. It is to note that apart from AWN, there is no overlap with proteins associated with boar fertility or semen preservability identified in previous high-throughput proteomics [17,31]. As stated above, varying results could be explained by different workflows of protein separation and identification procedures [17].…”

Section: Discussionmentioning

confidence: 88%

“…Besides this value for fertility, sperm-damaging effects were shown for the AQN-3 homologue and other heparin-binding spermadhesins [14,15], and presumably other secretions from the post sperm-rich fraction. Proteomic data of boar SP are now available [16] and links to semen preservability and fertility are being established [17]. Quantitative expression of particular SP proteins differs between ejaculate fractions [17][18][19] and is associated with differences in sperm quality [20][21][22], sperm freezability [23] and fertility [16,24,25].…”

Section: Introductionmentioning

confidence: 99%

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Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Höfner

Luther

Palladini

et al. 2020

IJMS

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Long-term exposure of liquid preserved boar spermatozoa to seminal plasma (SP) can cause dramatic sperm injury. This study examined whether boar specificity exists in the sensitivity of spermatozoa to SP and whether correspondent biomarkers can be identified. Consecutive ejaculates (n = 4–5) collected from 19 boars were centrifuged, diluted with a pH-stablising extender with 10% (v/v) autologous SP and evaluated by computer-assisted semen analysis and flow cytometry. Up until 144 h storage, four boars showed consistently high sperm motility, viability and mitochondria activity, and one boar showed consistently low values. Intra-boar variability was high in the other boars. Screening of SP (n = 12 samples) for protein markers using mass spectrometry identified three protein candidates of which the granulin precursor, legumain and AWN were 0.5 to 0.9 log2-fold less abundant (p < 0.05) in SP-resistant compared to SP-sensitive samples. Lipidome analysis by mass spectrometry revealed 568 lipids showing no difference between the SP-groups. The most abundant lipids were cholesterol (42,442 pmol), followed by phosphatidylserine (20,956 pmol) and ether-linked phosphatidylethanolamine (13,039 pmol). In conclusion, three candidate proteins were identified which might be indicative of SP-tolerance of sperm during long-term storage. Noteworthy, a first lipidomic profile of boar SP is presented.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Höfner

Luther

Palladini

et al. 2020

IJMS

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“…Artificial insemination (AI) is a central technique in modern pig breeding, and semen quality is the most important factor in determining offspring number and AI success. At present, boar semen is typically preserved using liquid storage or cryopreservation (Parrilla et al., 2019). However, when compared to sperms of other livestock, the spermatozoa of boar possess a relatively low cholesterol/phospholipid ratio, which renders them more sensitive to low temperatures and vulnerable to cold shock, which reduces sperm quality and pregnancy rates (Davis, 1981).…”

Section: Introductionmentioning

confidence: 99%

Notoginsenoside R1 protects boar sperm during liquid storage at 17°C

Ren

Feng

Niu

et al. 2020

Reprod Domestic Animals

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Reactive oxygen species (ROS) damage mammalian sperm during liquid storage. Notoginsenoside R1 (NR1) is a compound isolated from the roots of Panax notoginseng; it has powerful ROS‐scavenging activities. This work hypothesized that the antioxidant capacity of NR1 could improve boar sperm quality and fertility during liquid storage. During liquid storage at 17°C, the supplementation of semen extender with NR1 (50 μM) significantly improved sperm motility, membrane integrity and acrosome integrity after 5 days of preservation. NR1 treatment also reduced ROS and lipid peroxidation (LPO) levels at day 5 (p <0.05). Higher glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) levels and sperm–zona pellucida binding capacity were observed in the 50 μM NR1 group than those in the control group at day 7 (p <0.05). Importantly, statistical analysis of the fertility of 200 sows indicated that addition of NR1 to the extender improved the fertility parameters of boar spermatozoa during liquid storage at 17°C (p <0.05). These results demonstrate the practical feasibility of using 50 μM NR1 as an antioxidant in boar extender during liquid storage at 17°C, which is beneficial to both spermatozoa quality and fertility.

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“…Predicting the fertility outcome of cooled or frozen spermatozoa is one of the most relevant aspects in the field of porcine reproduction [5]. A recently published review summarizes the current knowledge about boar seminal plasma and the sperm proteome, focusing mainly on its relevance to sperm cryopreservation procedures and their outcomes in terms of sperm function and fertility [10]. With the use of comprehensive Multidimensional Protein Identification Technology (MudPIT) shotgun proteomic analysis a better understanding of mature boar spermatozoa was provided.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Fresh and Liquid-Stored Boar Spermatozoa

Bajuk

Zrimšek

Pipan

et al. 2020

Animals

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In this study comparative proteomics was used to define changes in the expression of the spermatozoa proteins during liquid storage. Semen from eight boars was analyzed on the day of collection and after liquid preservation at 15–17 °C for three days. Sperm parameters (concentration, motility, morphology, vitality) and percentage of non-capacitated and acrosomal-reacted spermatozoa were determined. Sperm proteins were extracted and separated by two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and proteomic profiles were computationally compared to highlight differentially expressed protein spots that were, in turn, identified by mass spectrometry. The intensities of four spots were significantly different between fresh and liquid stored sperm. Namely: ATP citrate lyase, chaperonin containing T-complex polypeptide 1 (TCP1) subunit ε and probable phospholipid-transporting ATP-ase were over-expressed in liquid stored sperm, whereas cytosolic non-specific dipeptidase was over-expressed in fresh sperm. These differentially expressed proteins could be used as plausible biomarkers for the evaluation of boar semen quality and spermatozoa survival after liquid storage and could help to address problems associated with sperm preservation.

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Boar semen proteomics and sperm preservation

Cited by 40 publications

References 46 publications

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Tolerance of Stored Boar Spermatozoa to Autologous Seminal Plasma: A Proteomic and Lipidomic Approach

Notoginsenoside R1 protects boar sperm during liquid storage at 17°C

Proteomic Analysis of Fresh and Liquid-Stored Boar Spermatozoa

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