BODIPY staining, an alternative to the Nile Red fluorescence method for the evaluation of intracellular lipids in microalgae

Govender, Thavendran; Ramanna, Luveshan; Rawat, Ismail; Bux, Faizal

doi:10.1016/j.biortech.2012.03.024

Cited by 211 publications

(160 citation statements)

References 32 publications

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“…The interval of 30 minutes was considered enough for average sorting gates (2-10 minutes) and longer sorting periods (if very narrow sorting gates are selected, 20 -30 minutes). The results showed an optimum concentration of 0.4 µg ml -1 for BP, which is close to previous results with other microalgae species 51,53,59 . Nonetheless, this factor is highly strain dependent, particularly due to cell wall thickness and membrane permeability 59 .…”

Section: Resultssupporting

confidence: 79%

“…DMSO is regarded as suitable and necessary for BP 52,65,66 , but likewise it is known for its toxicity at high concentrations 24,59 . Ethanol was used as a solvent of BP considering its overall low toxicity.…”

Section: Resultsmentioning

confidence: 99%

“…However, the retaining time of the NR fluorescence signal started to decrease substantially after 3 minutes in the stressed cells (Figure 3.2, p < 0.001). The stability of the dye is a crucial factor when using FACS 59 Pick et al 63 states that NR signal shifts between cytoplasmic plasma membrane and lipid globules fluorescence, and that such effect is more intense in cells with increased lipid content (i.e. N-starved).…”

Section: Discussionmentioning

confidence: 99%

“…Fluorescence of the Bodipy dye was used as a proxy for cellular lipid content 15,51,59,86 . BODIPY 505/515 is a dye used to stain non-polar lipid bodies in microalgae cells 15,51,52 .…”

Section: Growth Of Clittorale Before Cell Sortingmentioning

confidence: 99%

“…However, the ultimate inadequacy is its limited photo stability resulting in signal which is not constant in time. The application of BODIPY 505/515 (BP) probe in microalgae has become much more popular over the last few years 21,24,51,52,[57][58][59] . BODIPY 505/515 (4,4-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a-Diaza-sIndacene) has a high oil/water coefficient, which allows it to infuse membranes and makes the labelling of cell components faster than NR.…”

Section: Introductionmentioning

confidence: 99%

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The fatter the better : selecting microalgae cells for outdoor lipid production

Teles¹

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Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Fluorescence of the Bodipy dye was used as a proxy for cellular lipid content 15,51,59,86 . BODIPY 505/515 is a dye used to stain non-polar lipid bodies in microalgae cells 15,51,52 .…”

Section: Growth Of Clittorale Before Cell Sortingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The fatter the better : selecting microalgae cells for outdoor lipid production

Teles¹

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Current developments in high‐throughput analysis for microalgae cellular contents

Lee

Chang

Wang

2013

Biotechnology Journal

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Microalgae have emerged as one of the most promising feedstocks for biofuels and bio-based chemical production. However, due to the lack of effective tools enabling rapid and high-throughput analysis of the content of microalgae biomass, the efficiency of screening and identification of microalgae with desired functional components from the natural environment is usually quite low. Moreover, the real-time monitoring of the production of target components from microalgae is also difficult. Recently, research efforts focusing on overcoming this limitation have started. In this review, the recent development of high-throughput methods for analyzing microalgae cellular contents is summarized. The future prospects and impacts of these detection methods in microalgae-related processing and industries are also addressed.

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Microalgal swimming signatures and neutral lipids production across growth phases

You

Mallery

Mashek

et al. 2020

Biotech & Bioengineering

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Microalgae have been shown as a potential bioresource for food, biofuel, and pharmaceutical products. During the growth phases with corresponding environmental conditions, microalgae accumulate different amounts of various metabolites. We quantified the neutral lipids accumulation and analyzed the swimming signatures (speed and trajectories) of the motile green alga, Dunaliella primolecta, during the lag–exponential–stationary growth cycle at different nutrient concentrations. We discovered significant changes in the neutral lipid content and swimming signatures of microalgae across growth phases. The timing of the maximum swimming speed coincided with the maximum neutral lipid content and both maxima occurred under nutrient stress at the stationary growth phase. Furthermore, the swimming trajectories suggested statistically significant changes in swimming modes at the stationary growth phase when the maximum intracellular neutral lipid content was observed. Our results provide the potential exploitation of microalgal swimming signatures as possible indicators of the cultivation conditions and the timing of microalgal harvest to maximize the lipid yield for biofuel production. The findings can also be implemented to explore the production of food and antibiotics from other microalgal metabolites with low energy costs.

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BODIPY staining, an alternative to the Nile Red fluorescence method for the evaluation of intracellular lipids in microalgae

Cited by 211 publications

References 32 publications

The fatter the better : selecting microalgae cells for outdoor lipid production

The fatter the better : selecting microalgae cells for outdoor lipid production

Current developments in high‐throughput analysis for microalgae cellular contents

Microalgal swimming signatures and neutral lipids production across growth phases

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