Body Fat Content Can Be Predicted In Vivo in Mice Using a Modified Dual-Energy X-Ray Absorptiometry Technique

Sjögren, Klara; Hellberg, Nina; Bohlooly‐Y, Mohammad; Sävendahl, Lars; Johansson, Marie; Berglindh, T.; Bosæus, Ingvar; Ohlsson, Claes

doi:10.1093/jn/131.11.2963

Cited by 52 publications

(38 citation statements)

References 6 publications

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“…Clearly the results from the present study differ from that of Sjögren et al (8). The reason for the differences between studies is not clear.…”

Section: Discussioncontrasting

confidence: 99%

“…Thus, we concluded that 8 mm/s was a good trade-off between reproducibility and reasonable scan times. Sjögren et al (8) did not give any information on the DXA measurements (e.g., scan speed, resolution, histogram averaging method) nor did they provide data to substantiate their claim. Thus, if scan speeds in excess of 8 mm/s were used, that may explain their negative findings.…”

Section: Discussionmentioning

confidence: 99%

“…Sjögren et al (8) recently evaluated the Norland pDEXA Sabre for measuring body composition of mice. In their introduction they state that they "were unable to obtain any correlation between the amount of fat given by the software Figure 2: Effect of photoperiod on body weight of collared lemmings.…”

Section: Discussionmentioning

confidence: 99%

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Body Composition in a Seasonal Model of Obesity: Longitudinal Measures and Validation of DXA

Hunter

Nagy

2002

Obesity Research

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HUNTER, HEATHER L. AND TIM R. NAGY. Body composition in a seasonal model of obesity: longitudinal measures and validation of DXA. Obes Res. 2002;10: 1180 -1187. Objective: Collared lemmings, Dicrostonyx groenlandicus, show rapid changes in body mass on a seasonal basis. The objective of this study was to measure longitudinal changes in body composition in animals undergoing photoperiodinduced weight gain and loss using DXA. Research Methods and Procedures: Adult, female collared lemmings exposed to either long (LD; 22 hours light/2 hours dark) or short (SD; 8 hours light/16 hours dark) photoperiods were anesthetized, and DXA was used to determine fat mass, lean tissue mass (LTM), total-body bone mineral content, and total-bone mineral density. After a baseline scan, one-half of the animals were transferred to the alternate photoperiod (SD-LD, weight loss; LD-SD, weight gain) and one-half remained on the same photoperiod (controls; SD-SD, LD-LD). Body composition was determined by DXA after 4 and 8 weeks. Animals were killed, and body composition was determined by carcass analysis. DXA-derived data were validated by comparing with carcass analysis. Results: Body composition by DXA was highly related to body composition measured by chemical analysis, thereby justifying the use of DXA. Lemmings in the SD-LD group lost weight, and this was reflected in measurable losses of fat and LTM. Lemmings in the LD-SD group gained weight, which was shown by measurable increases in fat, LTM and total-body bone mineral content. Discussion: Comparison of body composition determined by DXA to that by chemical extraction revealed that DXA is useful for measuring body composition. The longitudinal analysis revealed that collared lemmings undergo rapid changes in body composition when exposed to changes in photoperiod.

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“…Clearly the results from the present study differ from that of Sjögren et al (8). The reason for the differences between studies is not clear.…”

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Body Composition in a Seasonal Model of Obesity: Longitudinal Measures and Validation of DXA

Hunter

Nagy

2002

Obesity Research

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“…Analysis of body composition. Analysis of body fat and lean body mass (LBM) in IL-1RI Ϫ/Ϫ and wild-type controls was performed by dual-energy X-ray absorptiometry (DEXA) with the Norland (Fort Atkinson, WI) pDEXA Sabre and the Sabre Research software (version 3.9.2), as described (17,18). The crown-rump length was measured on the same DEXA scans as the body fat and LBM and was defined as the distance between the crown of the skull to a point located in the middle line of the two caput femoris.…”

Section: Methodsmentioning

confidence: 99%

Mature-Onset Obesity in Interleukin-1 Receptor I Knockout Mice

et al. 2006

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Interleukin-1 (IL-1) is a major mediator of inflammation that exerts its biological activities through the IL-1 type I receptor (IL-1RI). The body weights of IL-1RI؊/؊ mice of both sexes started to deviate from those of wild-type mice at 5-6 months of age and were 20% higher at 9 months of age. Visceral and subcutaneous fat mass, measured by dual-energy X-ray absorptiometry and magnetic resonance imaging, was markedly (1.5-to 2.5-fold) increased. Lean body mass and crown-rump length were also slightly (11 and 5%, respectively) increased, as was serum IGF-I. Obese IL-1RI؊/؊ mice were insulin resistant, as evidenced by hyperinsulinemia, decreased glucose tolerance, and insulin sensitivity. To elucidate the mechanisms for the development of obesity, young preobese IL-1RI ؊/؊ mice were investigated. They showed decreased suppression of body weight and food intake in response to systemic leptin treatment. The decreased leptin responsiveness was even more pronounced in older obese animals. Moreover, spontaneous locomotor activity and fat utilization, as measured by respiratory quotient, were decreased in preobese IL-1RI ؊/؊ mice. In conclusion, lack of IL-1RI-mediated biological activity causes mature-onset obesity. This obese phenotype is preceded by decreased leptin sensitivity, fat utilization, and locomotor activity. Diabetes 55: [1205][1206][1207][1208][1209][1210][1211][1212][1213] 2006 T he prevalence of obesity is growing rapidly in many parts of the world and reaching epidemic proportions in several developed countries (1). Overweight and obesity are associated with increased risk of metabolic disorders, such as type 2 diabetes and hyperlipidemia, and thereby increased risk of cardiovascular mortality. Although the regulation of body weight and body composition involves input from lifestyle and environment, compelling scientific evidence indicates that propensity to develop obesity is in large part attributable to genetic factors. Over the last decade, the study of different transgenic and knockout mouse models has contributed to the identification of new factors involved in the complex mechanisms regulating energy balance and to the clarification of the contribution of genetics to obesity (2,3). Moreover, a number of human genes have been identified in which major missense or nonsense mutations as well as genetic variations are associated with obesityrelated phenotypes. Many of these genetic variants have occurred in molecules identical or similar to those identified as a cause of obesity in rodents, supporting their involvement in the regulation of body weight homeostasis also in humans (4).Interleukin-1 (IL-1) is a major mediator of inflammation and exerts effects on the neuro-immuno-endocrine system (5). Infection, injury, and inflammation are associated with negative energy balance, characterized by reduced food intake, weight loss, increased thermogenesis, and fever. IL-1 could be of importance for these effects, as peripheral or central injection of IL-1 induces a marked rise in body temperature (f...

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“…At week 15, body composition was measured in mice anesthetized with isofluorane (Baxter, Deerfield, IL, USA) using a Lunar PIXImus densitometer (Lunar Corp, Madison, WI, USA) as previously described (26).…”

Section: Dual Energy X-ray Absorptiometry (Dxa)mentioning

confidence: 99%

Dietary herring improves plasma lipid profiles and reduces atherosclerosis in obese low-density lipoprotein receptor-deficient mice

Gabrielsson

Wikström²,

Jakubowicz³

et al. 2011

Int J Mol Med

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Abstract. Diet is a significant modifiable risk factor for cardiovascular disease and high fish intake has been associated with vascular health in population studies. However, intervention studies have been inconclusive. In this study, male low-density lipoprotein receptor-deficient mice were given 16-week high fat/high sucrose diets, supplemented with either minced herring fillets or minced beef. The diets were matched in total fat and cholesterol content; taurine content and fatty acid composition was analysed. Body weights were recorded throughout the study; plasma lipids were analysed at week 8 and 16. Body composition and adipocyte size were evaluated at study end. Atherosclerosis was evaluated at week 12 (ultrasound) and at termination (en face histology). Herring-fed mice had a higher proportion of long-chain n-3 polyunsaturated fatty acids in the hepatic triacylglycerides (TAG) and phospholipid fractions. The herring-fed mice had increased body weight (P= 0.007), and reduced epididymal adipocyte size (P= 0.009), despite similar food intake and body composition as the beef-fed mice. The herring-fed mice had lower plasma TAG and verylow-density lipoprotein (VLDL)-cholesterol concentrations throughout the study (TAG; P= 0.0012 and 0.004, VLDLcholesterol; P=0.006 and 0.041, week 8 and 16, respectively). At week 16, the herring-fed had higher plasma concentrations of HDL-cholesterol (P=0.004) and less atherosclerotic lesions in the aortic arch (P=0.007) compared with the beef-fed mice.In conclusion, dietary herring in comparison to beef markedly improved vascular health in this mouse model, suggesting that herring provides an added value beyond its content of macronutrients.

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Body Fat Content Can Be Predicted In Vivo in Mice Using a Modified Dual-Energy X-Ray Absorptiometry Technique

Cited by 52 publications

References 6 publications

Body Composition in a Seasonal Model of Obesity: Longitudinal Measures and Validation of DXA

Body Composition in a Seasonal Model of Obesity: Longitudinal Measures and Validation of DXA

Mature-Onset Obesity in Interleukin-1 Receptor I Knockout Mice

Dietary herring improves plasma lipid profiles and reduces atherosclerosis in obese low-density lipoprotein receptor-deficient mice

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